Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF527 and GFF528 are separated by 89 nucleotides GFF528 and GFF529 are separated by 55 nucleotides GFF529 and GFF530 are separated by 51 nucleotides
GFF527 - FIG01045128: hypothetical protein, at 260,120 to 261,244
GFF527
GFF528 - Nickel responsive regulator NikR, at 261,334 to 261,771
GFF528
GFF529 - '4'-phosphopantetheinyl transferase (EC 2.7.8.-)' transl_table=11, at 261,827 to 262,405
GFF529
GFF530 - Putative PerM family permease, at 262,457 to 263,506
GFF530
Position (kb) 261
262
263 Strain fitness (log2 ratio) -2
-1
0
1 at 260.985 kb on + strand, within GFF527 at 260.985 kb on + strand, within GFF527 at 260.988 kb on + strand, within GFF527 at 260.988 kb on + strand, within GFF527 at 261.013 kb on - strand, within GFF527 at 261.050 kb on - strand, within GFF527 at 261.070 kb on + strand, within GFF527 at 261.073 kb on + strand, within GFF527 at 261.242 kb on + strand at 261.305 kb on + strand at 261.412 kb on + strand, within GFF528 at 261.413 kb on - strand, within GFF528 at 261.413 kb on - strand, within GFF528 at 261.413 kb on - strand, within GFF528 at 261.468 kb on + strand, within GFF528 at 261.660 kb on + strand, within GFF528 at 261.790 kb on + strand at 261.791 kb on - strand at 262.013 kb on - strand, within GFF529 at 262.174 kb on + strand, within GFF529 at 262.175 kb on - strand, within GFF529 at 262.248 kb on + strand, within GFF529 at 262.401 kb on + strand at 262.401 kb on + strand at 262.402 kb on - strand at 262.405 kb on + strand at 262.406 kb on - strand at 262.406 kb on - strand at 262.406 kb on - strand at 262.406 kb on - strand at 262.406 kb on - strand at 262.425 kb on - strand at 262.443 kb on + strand at 262.444 kb on - strand at 262.496 kb on + strand at 262.592 kb on + strand, within GFF530 at 262.746 kb on - strand, within GFF530 at 262.795 kb on + strand, within GFF530 at 262.795 kb on + strand, within GFF530 at 262.804 kb on - strand, within GFF530 at 262.804 kb on - strand, within GFF530 at 262.823 kb on + strand, within GFF530 at 262.877 kb on - strand, within GFF530 at 262.892 kb on + strand, within GFF530 at 262.921 kb on - strand, within GFF530 at 262.996 kb on + strand, within GFF530 at 263.087 kb on + strand, within GFF530 at 263.088 kb on - strand, within GFF530 at 263.138 kb on - strand, within GFF530 at 263.152 kb on + strand, within GFF530 at 263.152 kb on + strand, within GFF530 at 263.156 kb on + strand, within GFF530 at 263.230 kb on + strand, within GFF530 at 263.237 kb on - strand, within GFF530 at 263.279 kb on - strand, within GFF530 at 263.312 kb on - strand, within GFF530
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 260,985 + GFF527 0.77 -0.5 260,985 + GFF527 0.77 -0.4 260,988 + GFF527 0.77 +0.3 260,988 + GFF527 0.77 +0.2 261,013 - GFF527 0.79 +0.2 261,050 - GFF527 0.83 -0.6 261,070 + GFF527 0.84 -0.2 261,073 + GFF527 0.85 -0.6 261,242 + +0.2 261,305 + -0.4 261,412 + GFF528 0.18 +0.6 261,413 - GFF528 0.18 +0.5 261,413 - GFF528 0.18 +0.4 261,413 - GFF528 0.18 -0.3 261,468 + GFF528 0.31 -0.1 261,660 + GFF528 0.74 +0.7 261,790 + -0.8 261,791 - +0.3 262,013 - GFF529 0.32 -0.4 262,174 + GFF529 0.60 +1.1 262,175 - GFF529 0.60 +1.6 262,248 + GFF529 0.73 -0.3 262,401 + -0.4 262,401 + +1.0 262,402 - -0.0 262,405 + -0.3 262,406 - +0.5 262,406 - -0.5 262,406 - +0.3 262,406 - +1.6 262,406 - +0.1 262,425 - +0.5 262,443 + -0.1 262,444 - +0.9 262,496 + +1.2 262,592 + GFF530 0.13 +0.1 262,746 - GFF530 0.28 +0.4 262,795 + GFF530 0.32 -0.1 262,795 + GFF530 0.32 -0.9 262,804 - GFF530 0.33 +0.4 262,804 - GFF530 0.33 -0.4 262,823 + GFF530 0.35 +0.7 262,877 - GFF530 0.40 -0.0 262,892 + GFF530 0.41 -0.1 262,921 - GFF530 0.44 -0.0 262,996 + GFF530 0.51 +0.1 263,087 + GFF530 0.60 -1.8 263,088 - GFF530 0.60 -0.2 263,138 - GFF530 0.65 -0.3 263,152 + GFF530 0.66 +1.0 263,152 + GFF530 0.66 -0.8 263,156 + GFF530 0.67 -0.5 263,230 + GFF530 0.74 +0.0 263,237 - GFF530 0.74 +0.6 263,279 - GFF530 0.78 +0.3 263,312 - GFF530 0.81 +0.2
Or see this region's nucleotide sequence