Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF500 and GFF501 are separated by 39 nucleotides GFF501 and GFF502 are separated by 350 nucleotides
GFF500 - Uncharacterized protein YhjG, at 226,604 to 228,664
GFF500
GFF501 - Inner membrane metabolite transport protein YhjE, at 228,704 to 230,026
GFF501
GFF502 - Inner membrane protein YhjD, at 230,377 to 231,405
GFF502
Position (kb) 228
229
230
231 Strain fitness (log2 ratio) -1
0
1
2 at 227.940 kb on + strand, within GFF500 at 227.940 kb on + strand, within GFF500 at 227.946 kb on + strand, within GFF500 at 227.980 kb on + strand, within GFF500 at 227.980 kb on + strand, within GFF500 at 228.039 kb on + strand, within GFF500 at 228.039 kb on + strand, within GFF500 at 228.172 kb on + strand, within GFF500 at 228.254 kb on + strand, within GFF500 at 228.254 kb on + strand, within GFF500 at 228.255 kb on - strand, within GFF500 at 228.323 kb on - strand, within GFF500 at 228.423 kb on + strand, within GFF500 at 228.442 kb on + strand, within GFF500 at 228.457 kb on + strand, within GFF500 at 228.828 kb on + strand at 228.829 kb on - strand at 228.889 kb on - strand, within GFF501 at 228.909 kb on - strand, within GFF501 at 228.983 kb on + strand, within GFF501 at 229.157 kb on + strand, within GFF501 at 229.206 kb on + strand, within GFF501 at 229.207 kb on - strand, within GFF501 at 229.207 kb on - strand, within GFF501 at 229.258 kb on + strand, within GFF501 at 229.262 kb on - strand, within GFF501 at 229.322 kb on + strand, within GFF501 at 229.323 kb on - strand, within GFF501 at 229.836 kb on - strand, within GFF501 at 229.836 kb on - strand, within GFF501 at 229.873 kb on + strand, within GFF501 at 229.906 kb on - strand at 229.910 kb on - strand at 229.912 kb on - strand at 229.954 kb on + strand at 230.283 kb on + strand at 230.378 kb on + strand at 230.417 kb on + strand at 230.547 kb on - strand, within GFF502 at 230.812 kb on + strand, within GFF502 at 230.847 kb on - strand, within GFF502 at 230.988 kb on + strand, within GFF502 at 231.009 kb on + strand, within GFF502
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 227,940 + GFF500 0.65 -0.1 227,940 + GFF500 0.65 -0.5 227,946 + GFF500 0.65 -0.0 227,980 + GFF500 0.67 -0.3 227,980 + GFF500 0.67 -0.4 228,039 + GFF500 0.70 +0.4 228,039 + GFF500 0.70 -0.7 228,172 + GFF500 0.76 +0.3 228,254 + GFF500 0.80 +0.4 228,254 + GFF500 0.80 +2.0 228,255 - GFF500 0.80 -1.6 228,323 - GFF500 0.83 +0.2 228,423 + GFF500 0.88 -0.7 228,442 + GFF500 0.89 +0.6 228,457 + GFF500 0.90 +0.1 228,828 + +0.8 228,829 - -0.4 228,889 - GFF501 0.14 -0.3 228,909 - GFF501 0.15 +0.0 228,983 + GFF501 0.21 -0.1 229,157 + GFF501 0.34 -1.5 229,206 + GFF501 0.38 -0.3 229,207 - GFF501 0.38 +0.7 229,207 - GFF501 0.38 -0.3 229,258 + GFF501 0.42 -0.8 229,262 - GFF501 0.42 -0.3 229,322 + GFF501 0.47 +1.1 229,323 - GFF501 0.47 +0.1 229,836 - GFF501 0.86 +0.1 229,836 - GFF501 0.86 +0.3 229,873 + GFF501 0.88 -0.6 229,906 - +1.3 229,910 - -0.1 229,912 - -0.0 229,954 + +1.4 230,283 + +0.4 230,378 + +1.1 230,417 + +0.9 230,547 - GFF502 0.17 -0.8 230,812 + GFF502 0.42 -0.1 230,847 - GFF502 0.46 +1.3 230,988 + GFF502 0.59 +0.8 231,009 + GFF502 0.61 +0.1
Or see this region's nucleotide sequence