Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4902 and GFF4903 are separated by 323 nucleotides GFF4903 and GFF4904 are separated by 129 nucleotides GFF4904 and GFF4905 overlap by 4 nucleotides
GFF4902 - ATP-dependent Clp protease adaptor protein ClpS, at 2,409 to 2,729
GFF4902
GFF4903 - Cold shock protein CspD, at 3,053 to 3,274
GFF4903
GFF4904 - Macrolide export ATP-binding/permease protein MacB (EC 3.6.3.-), at 3,404 to 5,350
GFF4904
GFF4905 - Macrolide-specific efflux protein MacA, at 5,347 to 6,465
GFF4905
Position (kb)
3
4
5
6 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2.459 kb on - strand, within GFF4902 at 2.459 kb on - strand, within GFF4902 at 2.566 kb on - strand, within GFF4902 at 2.572 kb on + strand, within GFF4902 at 2.601 kb on - strand, within GFF4902 at 2.620 kb on + strand, within GFF4902 at 2.621 kb on - strand, within GFF4902 at 2.634 kb on + strand, within GFF4902 at 2.652 kb on - strand, within GFF4902 at 2.789 kb on + strand at 2.790 kb on - strand at 2.822 kb on + strand at 2.823 kb on - strand at 2.823 kb on - strand at 2.872 kb on + strand at 2.872 kb on + strand at 3.021 kb on - strand at 3.021 kb on - strand at 3.021 kb on - strand at 3.024 kb on + strand at 3.025 kb on - strand at 3.025 kb on - strand at 3.026 kb on + strand at 3.035 kb on + strand at 3.069 kb on + strand at 3.178 kb on + strand, within GFF4903 at 3.495 kb on - strand at 3.563 kb on - strand at 3.644 kb on + strand, within GFF4904 at 3.645 kb on - strand, within GFF4904 at 3.645 kb on - strand, within GFF4904 at 3.782 kb on + strand, within GFF4904 at 3.794 kb on - strand, within GFF4904 at 3.796 kb on - strand, within GFF4904 at 3.883 kb on + strand, within GFF4904 at 3.883 kb on + strand, within GFF4904 at 3.884 kb on - strand, within GFF4904 at 3.944 kb on - strand, within GFF4904 at 3.944 kb on - strand, within GFF4904 at 3.982 kb on + strand, within GFF4904 at 4.020 kb on + strand, within GFF4904 at 4.020 kb on + strand, within GFF4904 at 4.021 kb on - strand, within GFF4904 at 4.021 kb on - strand, within GFF4904 at 4.066 kb on + strand, within GFF4904 at 4.066 kb on + strand, within GFF4904 at 4.067 kb on - strand, within GFF4904 at 4.212 kb on + strand, within GFF4904 at 4.244 kb on - strand, within GFF4904 at 4.259 kb on - strand, within GFF4904 at 4.354 kb on - strand, within GFF4904 at 4.423 kb on + strand, within GFF4904 at 4.504 kb on + strand, within GFF4904 at 4.505 kb on - strand, within GFF4904 at 4.532 kb on + strand, within GFF4904 at 4.627 kb on + strand, within GFF4904 at 4.627 kb on + strand, within GFF4904 at 4.631 kb on + strand, within GFF4904 at 4.632 kb on - strand, within GFF4904 at 4.765 kb on + strand, within GFF4904 at 4.765 kb on + strand, within GFF4904 at 4.766 kb on - strand, within GFF4904 at 4.766 kb on - strand, within GFF4904 at 5.014 kb on - strand, within GFF4904 at 5.080 kb on + strand, within GFF4904 at 5.195 kb on - strand at 5.311 kb on - strand at 5.314 kb on + strand at 5.314 kb on + strand at 5.315 kb on - strand at 5.494 kb on - strand, within GFF4905 at 5.494 kb on - strand, within GFF4905 at 5.571 kb on + strand, within GFF4905 at 5.572 kb on - strand, within GFF4905 at 5.573 kb on + strand, within GFF4905 at 5.574 kb on - strand, within GFF4905 at 5.974 kb on + strand, within GFF4905 at 5.974 kb on + strand, within GFF4905 at 5.974 kb on + strand, within GFF4905 at 5.974 kb on + strand, within GFF4905 at 5.975 kb on - strand, within GFF4905 at 5.975 kb on - strand, within GFF4905 at 6.054 kb on + strand, within GFF4905 at 6.059 kb on - strand, within GFF4905 at 6.097 kb on + strand, within GFF4905 at 6.200 kb on - strand, within GFF4905 at 6.214 kb on - strand, within GFF4905
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 2,459 - GFF4902 0.16 +1.5 2,459 - GFF4902 0.16 +0.5 2,566 - GFF4902 0.49 +0.5 2,572 + GFF4902 0.51 -0.8 2,601 - GFF4902 0.60 -0.7 2,620 + GFF4902 0.66 -0.3 2,621 - GFF4902 0.66 +1.3 2,634 + GFF4902 0.70 +0.0 2,652 - GFF4902 0.76 +0.7 2,789 + +0.1 2,790 - -0.5 2,822 + +0.1 2,823 - -0.7 2,823 - -0.4 2,872 + -0.9 2,872 + +0.1 3,021 - +0.7 3,021 - +0.3 3,021 - +0.3 3,024 + +2.1 3,025 - -0.3 3,025 - -0.9 3,026 + +0.7 3,035 + -0.2 3,069 + -0.5 3,178 + GFF4903 0.56 -1.5 3,495 - -1.1 3,563 - +0.0 3,644 + GFF4904 0.12 +2.6 3,645 - GFF4904 0.12 -0.4 3,645 - GFF4904 0.12 +1.7 3,782 + GFF4904 0.19 +0.1 3,794 - GFF4904 0.20 +1.8 3,796 - GFF4904 0.20 -0.7 3,883 + GFF4904 0.25 +2.8 3,883 + GFF4904 0.25 -0.7 3,884 - GFF4904 0.25 -0.1 3,944 - GFF4904 0.28 +0.8 3,944 - GFF4904 0.28 +0.9 3,982 + GFF4904 0.30 +0.4 4,020 + GFF4904 0.32 -0.1 4,020 + GFF4904 0.32 +1.1 4,021 - GFF4904 0.32 -0.1 4,021 - GFF4904 0.32 -1.4 4,066 + GFF4904 0.34 -0.7 4,066 + GFF4904 0.34 +0.2 4,067 - GFF4904 0.34 +0.6 4,212 + GFF4904 0.41 +0.2 4,244 - GFF4904 0.43 -1.1 4,259 - GFF4904 0.44 -2.1 4,354 - GFF4904 0.49 -1.1 4,423 + GFF4904 0.52 -0.1 4,504 + GFF4904 0.56 -0.8 4,505 - GFF4904 0.57 +0.2 4,532 + GFF4904 0.58 -0.1 4,627 + GFF4904 0.63 -0.2 4,627 + GFF4904 0.63 -0.9 4,631 + GFF4904 0.63 +1.4 4,632 - GFF4904 0.63 +0.1 4,765 + GFF4904 0.70 +0.9 4,765 + GFF4904 0.70 -0.3 4,766 - GFF4904 0.70 -0.3 4,766 - GFF4904 0.70 -0.1 5,014 - GFF4904 0.83 +0.1 5,080 + GFF4904 0.86 +1.5 5,195 - +0.0 5,311 - -0.6 5,314 + +0.5 5,314 + +0.1 5,315 - -1.8 5,494 - GFF4905 0.13 +0.7 5,494 - GFF4905 0.13 +1.7 5,571 + GFF4905 0.20 -0.5 5,572 - GFF4905 0.20 +0.2 5,573 + GFF4905 0.20 -0.1 5,574 - GFF4905 0.20 -0.3 5,974 + GFF4905 0.56 -0.8 5,974 + GFF4905 0.56 +0.3 5,974 + GFF4905 0.56 +0.1 5,974 + GFF4905 0.56 -1.2 5,975 - GFF4905 0.56 +0.1 5,975 - GFF4905 0.56 +0.3 6,054 + GFF4905 0.63 -0.7 6,059 - GFF4905 0.64 -0.1 6,097 + GFF4905 0.67 +0.3 6,200 - GFF4905 0.76 +0.3 6,214 - GFF4905 0.77 +0.2
Or see this region's nucleotide sequence