Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4843 and GFF4844 are separated by 82 nucleotides GFF4844 and GFF4845 are separated by 149 nucleotides GFF4845 and GFF4846 are separated by 18 nucleotides GFF4846 and GFF4847 overlap by 1 nucleotides
GFF4843 - D-glucarate permease, at 143,203 to 144,561
GFF4843
GFF4844 - FIG01046344: hypothetical protein, at 144,644 to 144,892
GFF4844
GFF4845 - Hypothetical flavoprotein YqcA (clustered with tRNA pseudouridine synthase C), at 145,042 to 145,491
GFF4845
GFF4846 - tRNA pseudouridine synthase C (EC 4.2.1.70), at 145,510 to 146,292
GFF4846
GFF4847 - Hypothetical protein YqcC (clustered with tRNA pseudouridine synthase C), at 146,292 to 146,621
GFF4847
Position (kb) 145
146 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 144.208 kb on - strand, within GFF4843 at 144.237 kb on + strand, within GFF4843 at 144.550 kb on + strand at 144.551 kb on - strand at 144.587 kb on - strand at 144.587 kb on - strand at 144.591 kb on - strand at 144.632 kb on - strand at 144.658 kb on - strand at 144.658 kb on - strand at 144.707 kb on + strand, within GFF4844 at 144.710 kb on - strand, within GFF4844 at 144.765 kb on + strand, within GFF4844 at 144.927 kb on + strand at 144.928 kb on - strand at 144.986 kb on + strand at 145.051 kb on + strand at 145.124 kb on - strand, within GFF4845 at 145.217 kb on - strand, within GFF4845 at 145.260 kb on + strand, within GFF4845 at 145.261 kb on - strand, within GFF4845 at 145.267 kb on - strand, within GFF4845 at 145.344 kb on + strand, within GFF4845 at 145.344 kb on + strand, within GFF4845 at 145.458 kb on - strand at 145.458 kb on - strand at 145.458 kb on - strand at 145.492 kb on + strand at 145.493 kb on - strand at 145.512 kb on - strand at 145.770 kb on + strand, within GFF4846 at 146.277 kb on - strand at 146.300 kb on + strand at 146.389 kb on + strand, within GFF4847 at 146.390 kb on - strand, within GFF4847 at 146.447 kb on + strand, within GFF4847 at 146.447 kb on + strand, within GFF4847 at 146.447 kb on + strand, within GFF4847 at 146.448 kb on - strand, within GFF4847 at 146.448 kb on - strand, within GFF4847 at 146.448 kb on - strand, within GFF4847
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 144,208 - GFF4843 0.74 +0.6 144,237 + GFF4843 0.76 +0.9 144,550 + +0.2 144,551 - -1.1 144,587 - -0.1 144,587 - -2.0 144,591 - +2.8 144,632 - -0.9 144,658 - +0.3 144,658 - -0.0 144,707 + GFF4844 0.25 +1.5 144,710 - GFF4844 0.27 -0.5 144,765 + GFF4844 0.49 -0.5 144,927 + +0.1 144,928 - -0.3 144,986 + -0.0 145,051 + +0.6 145,124 - GFF4845 0.18 +1.3 145,217 - GFF4845 0.39 -0.2 145,260 + GFF4845 0.48 -0.8 145,261 - GFF4845 0.49 +1.1 145,267 - GFF4845 0.50 -0.7 145,344 + GFF4845 0.67 -0.4 145,344 + GFF4845 0.67 +0.5 145,458 - -1.4 145,458 - -0.1 145,458 - -1.1 145,492 + +0.2 145,493 - -0.0 145,512 - +0.8 145,770 + GFF4846 0.33 -0.3 146,277 - -0.5 146,300 + +1.4 146,389 + GFF4847 0.29 +0.5 146,390 - GFF4847 0.30 -0.3 146,447 + GFF4847 0.47 +0.0 146,447 + GFF4847 0.47 -0.7 146,447 + GFF4847 0.47 -1.3 146,448 - GFF4847 0.47 -0.1 146,448 - GFF4847 0.47 -0.4 146,448 - GFF4847 0.47 -0.9
Or see this region's nucleotide sequence