Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF461 and GFF462 overlap by 32 nucleotides GFF462 and GFF463 overlap by 23 nucleotides GFF463 and GFF464 are separated by 156 nucleotides
GFF461 - Anaerobic dimethyl sulfoxide reductase chain A (EC 1.8.5.3), at 180,425 to 182,758
GFF461
GFF462 - Acetyltransferase (EC 2.3.1.-), at 182,727 to 183,167
GFF462
GFF463 - DNA-3-methyladenine glycosylase (EC 3.2.2.20), at 183,145 to 183,726
GFF463
GFF464 - FIG00543870: hypothetical protein, at 183,883 to 184,587
GFF464
Position (kb) 182
183
184 Strain fitness (log2 ratio) -1
0
1
2
3 at 181.843 kb on + strand, within GFF461 at 181.844 kb on - strand, within GFF461 at 181.848 kb on - strand, within GFF461 at 181.848 kb on - strand, within GFF461 at 181.880 kb on + strand, within GFF461 at 181.881 kb on - strand, within GFF461 at 181.881 kb on - strand, within GFF461 at 181.882 kb on + strand, within GFF461 at 181.882 kb on + strand, within GFF461 at 181.882 kb on + strand, within GFF461 at 181.883 kb on - strand, within GFF461 at 181.883 kb on - strand, within GFF461 at 181.992 kb on - strand, within GFF461 at 182.253 kb on - strand, within GFF461 at 182.253 kb on - strand, within GFF461 at 182.378 kb on - strand, within GFF461 at 182.381 kb on + strand, within GFF461 at 182.381 kb on + strand, within GFF461 at 182.382 kb on - strand, within GFF461 at 182.757 kb on - strand at 183.034 kb on - strand, within GFF462 at 183.037 kb on + strand, within GFF462 at 183.100 kb on + strand, within GFF462 at 183.101 kb on - strand, within GFF462 at 183.101 kb on - strand, within GFF462 at 183.131 kb on + strand at 183.131 kb on + strand at 183.132 kb on - strand at 183.202 kb on - strand at 183.202 kb on - strand at 183.231 kb on + strand, within GFF463 at 183.516 kb on + strand, within GFF463 at 183.517 kb on - strand, within GFF463 at 183.687 kb on + strand at 183.687 kb on + strand at 183.687 kb on + strand at 183.688 kb on - strand at 183.689 kb on + strand at 183.689 kb on + strand at 183.690 kb on - strand at 183.690 kb on - strand at 183.690 kb on - strand at 183.726 kb on + strand at 183.727 kb on - strand at 183.727 kb on - strand at 183.799 kb on + strand at 183.800 kb on - strand at 183.803 kb on + strand at 183.806 kb on + strand at 183.845 kb on - strand at 183.847 kb on + strand at 183.890 kb on + strand at 183.961 kb on - strand, within GFF464 at 183.967 kb on + strand, within GFF464 at 183.967 kb on + strand, within GFF464 at 183.985 kb on + strand, within GFF464 at 183.986 kb on - strand, within GFF464 at 184.025 kb on - strand, within GFF464 at 184.114 kb on - strand, within GFF464
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 181,843 + GFF461 0.61 +3.6 181,844 - GFF461 0.61 +0.1 181,848 - GFF461 0.61 +0.1 181,848 - GFF461 0.61 +0.0 181,880 + GFF461 0.62 -0.9 181,881 - GFF461 0.62 -0.5 181,881 - GFF461 0.62 -0.3 181,882 + GFF461 0.62 -0.3 181,882 + GFF461 0.62 +1.1 181,882 + GFF461 0.62 -0.1 181,883 - GFF461 0.62 -0.1 181,883 - GFF461 0.62 +1.4 181,992 - GFF461 0.67 -0.1 182,253 - GFF461 0.78 +0.2 182,253 - GFF461 0.78 +0.0 182,378 - GFF461 0.84 +0.8 182,381 + GFF461 0.84 -0.5 182,381 + GFF461 0.84 +0.0 182,382 - GFF461 0.84 -0.2 182,757 - +1.1 183,034 - GFF462 0.70 -0.5 183,037 + GFF462 0.70 -0.8 183,100 + GFF462 0.85 -0.5 183,101 - GFF462 0.85 -0.5 183,101 - GFF462 0.85 -0.8 183,131 + -0.5 183,131 + +2.7 183,132 - +1.0 183,202 - -0.3 183,202 - +0.2 183,231 + GFF463 0.15 +0.8 183,516 + GFF463 0.64 -0.3 183,517 - GFF463 0.64 -0.1 183,687 + +0.3 183,687 + -0.4 183,687 + +0.6 183,688 - +0.0 183,689 + +0.0 183,689 + +2.2 183,690 - +0.2 183,690 - +0.1 183,690 - -0.0 183,726 + -0.2 183,727 - -0.1 183,727 - -0.4 183,799 + +2.5 183,800 - -0.1 183,803 + -0.7 183,806 + -0.3 183,845 - +0.1 183,847 + -0.1 183,890 + -0.5 183,961 - GFF464 0.11 +0.1 183,967 + GFF464 0.12 -0.3 183,967 + GFF464 0.12 -0.3 183,985 + GFF464 0.14 -1.1 183,986 - GFF464 0.15 -0.5 184,025 - GFF464 0.20 -0.2 184,114 - GFF464 0.33 -1.1
Or see this region's nucleotide sequence