Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4482 and GFF4483 are separated by 96 nucleotides GFF4483 and GFF4484 overlap by 1 nucleotides GFF4484 and GFF4485 are separated by 288 nucleotides GFF4485 and GFF4486 are separated by 5 nucleotides GFF4486 and GFF4487 are separated by 90 nucleotides
GFF4482 - Oxygen-insensitive NAD(P)H nitroreductase (EC 1.-.-.-) / Dihydropteridine reductase (EC 1.5.1.34), at 267,242 to 267,895
GFF4482
GFF4483 - FIG074102: hypothetical protein, at 267,992 to 268,360
GFF4483
GFF4484 - Transcriptional regulator, TetR family, at 268,360 to 268,941
GFF4484
GFF4485 - Transcriptional activator RamA, at 269,230 to 269,571
GFF4485
GFF4486 - Putative inner membrane protein, at 269,577 to 269,801
GFF4486
GFF4487 - FIG00638355: hypothetical protein, at 269,892 to 271,010
GFF4487
Position (kb) 268
269 Strain fitness (log2 ratio) -1
0
1
2 at 267.466 kb on - strand, within GFF4482 at 267.556 kb on + strand, within GFF4482 at 267.694 kb on - strand, within GFF4482 at 267.795 kb on - strand, within GFF4482 at 267.806 kb on + strand, within GFF4482 at 267.873 kb on - strand at 267.895 kb on + strand at 267.896 kb on - strand at 267.915 kb on - strand at 268.090 kb on + strand, within GFF4483 at 268.127 kb on - strand, within GFF4483 at 268.173 kb on - strand, within GFF4483 at 268.272 kb on + strand, within GFF4483 at 268.272 kb on + strand, within GFF4483 at 268.272 kb on + strand, within GFF4483 at 268.413 kb on + strand at 268.415 kb on + strand at 268.515 kb on + strand, within GFF4484 at 268.515 kb on + strand, within GFF4484 at 268.566 kb on + strand, within GFF4484 at 268.567 kb on - strand, within GFF4484 at 268.668 kb on - strand, within GFF4484 at 268.767 kb on - strand, within GFF4484 at 269.040 kb on - strand at 269.101 kb on + strand at 269.157 kb on - strand at 269.166 kb on - strand at 269.281 kb on - strand, within GFF4485 at 269.298 kb on - strand, within GFF4485 at 269.311 kb on - strand, within GFF4485 at 269.358 kb on + strand, within GFF4485 at 269.365 kb on + strand, within GFF4485 at 269.366 kb on - strand, within GFF4485 at 269.408 kb on + strand, within GFF4485 at 269.409 kb on - strand, within GFF4485 at 269.457 kb on + strand, within GFF4485 at 269.457 kb on + strand, within GFF4485 at 269.884 kb on + strand at 269.884 kb on + strand at 269.885 kb on - strand at 269.934 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 267,466 - GFF4482 0.34 -1.5 267,556 + GFF4482 0.48 -0.6 267,694 - GFF4482 0.69 -0.3 267,795 - GFF4482 0.85 +0.4 267,806 + GFF4482 0.86 +0.6 267,873 - +1.9 267,895 + +0.1 267,896 - -1.7 267,915 - -0.2 268,090 + GFF4483 0.27 +0.5 268,127 - GFF4483 0.37 +0.2 268,173 - GFF4483 0.49 -0.0 268,272 + GFF4483 0.76 -0.4 268,272 + GFF4483 0.76 -0.4 268,272 + GFF4483 0.76 +0.2 268,413 + +0.2 268,415 + +0.7 268,515 + GFF4484 0.27 +2.4 268,515 + GFF4484 0.27 +0.5 268,566 + GFF4484 0.35 +0.4 268,567 - GFF4484 0.36 +0.0 268,668 - GFF4484 0.53 -0.5 268,767 - GFF4484 0.70 -0.5 269,040 - -0.3 269,101 + +1.1 269,157 - -0.2 269,166 - +0.1 269,281 - GFF4485 0.15 +0.7 269,298 - GFF4485 0.20 +0.6 269,311 - GFF4485 0.24 +0.2 269,358 + GFF4485 0.37 -0.4 269,365 + GFF4485 0.39 +0.6 269,366 - GFF4485 0.40 -0.3 269,408 + GFF4485 0.52 -0.2 269,409 - GFF4485 0.52 +0.2 269,457 + GFF4485 0.66 -0.8 269,457 + GFF4485 0.66 +0.9 269,884 + -0.3 269,884 + +1.2 269,885 - -0.1 269,934 + +0.7
Or see this region's nucleotide sequence