Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4480 and GFF4481 overlap by 23 nucleotides GFF4481 and GFF4482 are separated by 199 nucleotides GFF4482 and GFF4483 are separated by 96 nucleotides GFF4483 and GFF4484 overlap by 1 nucleotides
GFF4480 - PTS system, mannose-specific IIB component (EC 2.7.1.69), at 266,145 to 266,615
GFF4480
GFF4481 - PTS system, mannose-specific IIA component (EC 2.7.1.69), at 266,593 to 267,042
GFF4481
GFF4482 - Oxygen-insensitive NAD(P)H nitroreductase (EC 1.-.-.-) / Dihydropteridine reductase (EC 1.5.1.34), at 267,242 to 267,895
GFF4482
GFF4483 - FIG074102: hypothetical protein, at 267,992 to 268,360
GFF4483
GFF4484 - Transcriptional regulator, TetR family, at 268,360 to 268,941
GFF4484
Position (kb) 267
268 Strain fitness (log2 ratio) -1
0
1
2 at 266.257 kb on + strand, within GFF4480 at 266.258 kb on - strand, within GFF4480 at 266.260 kb on - strand, within GFF4480 at 266.363 kb on + strand, within GFF4480 at 266.478 kb on + strand, within GFF4480 at 266.479 kb on - strand, within GFF4480 at 266.487 kb on - strand, within GFF4480 at 266.604 kb on + strand at 266.630 kb on - strand at 266.653 kb on + strand, within GFF4481 at 266.700 kb on - strand, within GFF4481 at 266.936 kb on + strand, within GFF4481 at 266.991 kb on + strand, within GFF4481 at 267.030 kb on - strand at 267.030 kb on - strand at 267.146 kb on + strand at 267.349 kb on - strand, within GFF4482 at 267.466 kb on - strand, within GFF4482 at 267.556 kb on + strand, within GFF4482 at 267.694 kb on - strand, within GFF4482 at 267.795 kb on - strand, within GFF4482 at 267.806 kb on + strand, within GFF4482 at 267.873 kb on - strand at 267.895 kb on + strand at 267.896 kb on - strand at 267.915 kb on - strand at 268.090 kb on + strand, within GFF4483 at 268.127 kb on - strand, within GFF4483 at 268.173 kb on - strand, within GFF4483 at 268.272 kb on + strand, within GFF4483 at 268.272 kb on + strand, within GFF4483 at 268.272 kb on + strand, within GFF4483 at 268.413 kb on + strand at 268.415 kb on + strand at 268.515 kb on + strand, within GFF4484 at 268.515 kb on + strand, within GFF4484 at 268.566 kb on + strand, within GFF4484 at 268.567 kb on - strand, within GFF4484 at 268.668 kb on - strand, within GFF4484 at 268.767 kb on - strand, within GFF4484
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 266,257 + GFF4480 0.24 +0.5 266,258 - GFF4480 0.24 +0.1 266,260 - GFF4480 0.24 +0.1 266,363 + GFF4480 0.46 +0.3 266,478 + GFF4480 0.71 +0.4 266,479 - GFF4480 0.71 -0.3 266,487 - GFF4480 0.73 -0.4 266,604 + +0.0 266,630 - +0.5 266,653 + GFF4481 0.13 +0.8 266,700 - GFF4481 0.24 +0.7 266,936 + GFF4481 0.76 +0.9 266,991 + GFF4481 0.88 -0.4 267,030 - -0.7 267,030 - +1.7 267,146 + +0.2 267,349 - GFF4482 0.16 +0.3 267,466 - GFF4482 0.34 -1.5 267,556 + GFF4482 0.48 -0.6 267,694 - GFF4482 0.69 -0.3 267,795 - GFF4482 0.85 +0.4 267,806 + GFF4482 0.86 +0.6 267,873 - +1.9 267,895 + +0.1 267,896 - -1.7 267,915 - -0.2 268,090 + GFF4483 0.27 +0.5 268,127 - GFF4483 0.37 +0.2 268,173 - GFF4483 0.49 -0.0 268,272 + GFF4483 0.76 -0.4 268,272 + GFF4483 0.76 -0.4 268,272 + GFF4483 0.76 +0.2 268,413 + +0.2 268,415 + +0.7 268,515 + GFF4484 0.27 +2.4 268,515 + GFF4484 0.27 +0.5 268,566 + GFF4484 0.35 +0.4 268,567 - GFF4484 0.36 +0.0 268,668 - GFF4484 0.53 -0.5 268,767 - GFF4484 0.70 -0.5
Or see this region's nucleotide sequence