Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF447 and GFF448 are separated by 261 nucleotides GFF448 and GFF449 are separated by 138 nucleotides
GFF447 - Inner membrane protein YiaH, at 168,716 to 169,711
GFF447
GFF448 - Uncharacterized lipoprotein YsaB precursor, at 169,973 to 170,179
GFF448
GFF449 - Glycyl-tRNA synthetase alpha chain (EC 6.1.1.14), at 170,318 to 171,229
GFF449
Position (kb) 169
170
171 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 169.002 kb on + strand, within GFF447 at 169.003 kb on - strand, within GFF447 at 169.034 kb on + strand, within GFF447 at 169.061 kb on - strand, within GFF447 at 169.151 kb on - strand, within GFF447 at 169.164 kb on + strand, within GFF447 at 169.167 kb on + strand, within GFF447 at 169.167 kb on + strand, within GFF447 at 169.168 kb on - strand, within GFF447 at 169.189 kb on - strand, within GFF447 at 169.189 kb on - strand, within GFF447 at 169.189 kb on - strand, within GFF447 at 169.189 kb on - strand, within GFF447 at 169.246 kb on + strand, within GFF447 at 169.255 kb on + strand, within GFF447 at 169.329 kb on + strand, within GFF447 at 169.421 kb on - strand, within GFF447 at 169.478 kb on - strand, within GFF447 at 169.639 kb on + strand at 169.639 kb on + strand at 169.639 kb on + strand at 169.639 kb on + strand at 169.640 kb on - strand at 169.640 kb on - strand at 169.640 kb on - strand at 169.642 kb on + strand at 169.778 kb on - strand at 169.841 kb on + strand at 169.841 kb on + strand at 169.844 kb on + strand at 169.845 kb on - strand at 169.845 kb on - strand at 169.908 kb on + strand at 169.970 kb on - strand at 170.090 kb on + strand, within GFF448 at 170.090 kb on + strand, within GFF448 at 170.091 kb on - strand, within GFF448 at 170.091 kb on - strand, within GFF448 at 170.251 kb on - strand at 170.270 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 169,002 + GFF447 0.29 -0.5 169,003 - GFF447 0.29 +0.9 169,034 + GFF447 0.32 +0.5 169,061 - GFF447 0.35 +0.0 169,151 - GFF447 0.44 +3.7 169,164 + GFF447 0.45 -0.7 169,167 + GFF447 0.45 -0.3 169,167 + GFF447 0.45 +0.2 169,168 - GFF447 0.45 +1.5 169,189 - GFF447 0.47 +0.8 169,189 - GFF447 0.47 +0.3 169,189 - GFF447 0.47 +0.2 169,189 - GFF447 0.47 +0.2 169,246 + GFF447 0.53 -0.1 169,255 + GFF447 0.54 +0.1 169,329 + GFF447 0.62 +0.7 169,421 - GFF447 0.71 +4.3 169,478 - GFF447 0.77 +0.2 169,639 + -0.5 169,639 + -0.1 169,639 + -2.3 169,639 + +0.4 169,640 - -0.2 169,640 - -0.4 169,640 - -0.4 169,642 + +0.1 169,778 - -0.2 169,841 + +0.7 169,841 + +0.5 169,844 + -0.3 169,845 - -0.2 169,845 - -0.3 169,908 + +1.2 169,970 - -0.1 170,090 + GFF448 0.57 -0.5 170,090 + GFF448 0.57 -0.0 170,091 - GFF448 0.57 +0.1 170,091 - GFF448 0.57 -0.7 170,251 - +0.6 170,270 + -0.9
Or see this region's nucleotide sequence