Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF438 and GFF439 are separated by 173 nucleotides GFF439 and GFF440 are separated by 51 nucleotides GFF440 and GFF441 are separated by 381 nucleotides
GFF438 - Valine--pyruvate aminotransferase (EC 2.6.1.66), at 159,096 to 160,346
GFF438
GFF439 - Periplasmic alpha-amylase (EC 3.2.1.1), at 160,520 to 162,547
GFF439
GFF440 - hypothetical protein, at 162,599 to 162,733
GFF440
GFF441 - BAX protein, at 163,115 to 163,687
GFF441
Position (kb)
160
161
162
163 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 159.555 kb on - strand, within GFF438 at 159.618 kb on + strand, within GFF438 at 159.618 kb on + strand, within GFF438 at 159.618 kb on + strand, within GFF438 at 159.618 kb on + strand, within GFF438 at 159.619 kb on - strand, within GFF438 at 159.847 kb on + strand, within GFF438 at 159.847 kb on + strand, within GFF438 at 159.848 kb on - strand, within GFF438 at 159.848 kb on - strand, within GFF438 at 159.848 kb on - strand, within GFF438 at 159.851 kb on + strand, within GFF438 at 159.957 kb on + strand, within GFF438 at 159.960 kb on + strand, within GFF438 at 159.971 kb on + strand, within GFF438 at 160.004 kb on + strand, within GFF438 at 160.008 kb on + strand, within GFF438 at 160.143 kb on - strand, within GFF438 at 160.178 kb on + strand, within GFF438 at 160.178 kb on + strand, within GFF438 at 160.388 kb on + strand at 160.563 kb on + strand at 160.652 kb on - strand at 160.654 kb on + strand at 160.654 kb on + strand at 160.654 kb on + strand at 160.655 kb on - strand at 160.780 kb on - strand, within GFF439 at 161.010 kb on + strand, within GFF439 at 161.010 kb on + strand, within GFF439 at 161.068 kb on + strand, within GFF439 at 161.290 kb on - strand, within GFF439 at 161.318 kb on - strand, within GFF439 at 161.360 kb on + strand, within GFF439 at 161.460 kb on + strand, within GFF439 at 161.460 kb on + strand, within GFF439 at 161.549 kb on + strand, within GFF439 at 161.553 kb on + strand, within GFF439 at 161.554 kb on - strand, within GFF439 at 161.571 kb on + strand, within GFF439 at 161.618 kb on + strand, within GFF439 at 161.742 kb on + strand, within GFF439 at 161.837 kb on + strand, within GFF439 at 161.837 kb on + strand, within GFF439 at 161.837 kb on + strand, within GFF439 at 161.837 kb on + strand, within GFF439 at 161.841 kb on + strand, within GFF439 at 161.922 kb on + strand, within GFF439 at 161.922 kb on + strand, within GFF439 at 161.923 kb on - strand, within GFF439 at 161.923 kb on - strand, within GFF439 at 161.923 kb on - strand, within GFF439 at 161.923 kb on - strand, within GFF439 at 161.923 kb on - strand, within GFF439 at 161.977 kb on - strand, within GFF439 at 162.109 kb on - strand, within GFF439 at 162.109 kb on - strand, within GFF439 at 162.192 kb on + strand, within GFF439 at 162.262 kb on + strand, within GFF439 at 162.266 kb on + strand, within GFF439 at 162.266 kb on + strand, within GFF439 at 162.266 kb on + strand, within GFF439 at 162.266 kb on + strand, within GFF439 at 162.267 kb on - strand, within GFF439 at 162.267 kb on - strand, within GFF439 at 162.278 kb on - strand, within GFF439 at 162.278 kb on - strand, within GFF439 at 162.674 kb on - strand, within GFF440 at 162.737 kb on - strand at 162.772 kb on + strand at 162.867 kb on + strand at 162.904 kb on + strand at 162.904 kb on + strand at 162.905 kb on - strand at 162.910 kb on - strand at 162.981 kb on + strand at 162.995 kb on + strand at 163.012 kb on + strand at 163.022 kb on + strand at 163.023 kb on - strand at 163.122 kb on - strand at 163.148 kb on - strand at 163.181 kb on + strand, within GFF441 at 163.270 kb on + strand, within GFF441 at 163.270 kb on + strand, within GFF441 at 163.283 kb on - strand, within GFF441 at 163.375 kb on + strand, within GFF441 at 163.376 kb on - strand, within GFF441 at 163.421 kb on - strand, within GFF441 at 163.488 kb on - strand, within GFF441 at 163.518 kb on - strand, within GFF441
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 159,555 - GFF438 0.37 +0.4 159,618 + GFF438 0.42 -0.9 159,618 + GFF438 0.42 +1.4 159,618 + GFF438 0.42 -0.4 159,618 + GFF438 0.42 -0.3 159,619 - GFF438 0.42 -0.6 159,847 + GFF438 0.60 -0.1 159,847 + GFF438 0.60 +0.9 159,848 - GFF438 0.60 +1.5 159,848 - GFF438 0.60 -0.4 159,848 - GFF438 0.60 -1.2 159,851 + GFF438 0.60 +0.8 159,957 + GFF438 0.69 -0.1 159,960 + GFF438 0.69 +2.7 159,971 + GFF438 0.70 +0.4 160,004 + GFF438 0.73 -0.2 160,008 + GFF438 0.73 -0.1 160,143 - GFF438 0.84 -0.9 160,178 + GFF438 0.86 +1.3 160,178 + GFF438 0.86 -0.2 160,388 + -0.8 160,563 + +0.4 160,652 - -0.4 160,654 + +0.1 160,654 + -0.5 160,654 + -0.5 160,655 - +0.7 160,780 - GFF439 0.13 -0.0 161,010 + GFF439 0.24 +0.4 161,010 + GFF439 0.24 -0.2 161,068 + GFF439 0.27 +4.2 161,290 - GFF439 0.38 +0.3 161,318 - GFF439 0.39 +1.0 161,360 + GFF439 0.41 +0.0 161,460 + GFF439 0.46 -0.2 161,460 + GFF439 0.46 -0.7 161,549 + GFF439 0.51 -0.2 161,553 + GFF439 0.51 +0.1 161,554 - GFF439 0.51 -0.1 161,571 + GFF439 0.52 +1.8 161,618 + GFF439 0.54 +0.3 161,742 + GFF439 0.60 +1.6 161,837 + GFF439 0.65 -0.1 161,837 + GFF439 0.65 +0.5 161,837 + GFF439 0.65 +0.6 161,837 + GFF439 0.65 -0.1 161,841 + GFF439 0.65 -0.4 161,922 + GFF439 0.69 +0.5 161,922 + GFF439 0.69 +0.1 161,923 - GFF439 0.69 +1.1 161,923 - GFF439 0.69 +0.6 161,923 - GFF439 0.69 -0.0 161,923 - GFF439 0.69 -0.5 161,923 - GFF439 0.69 +2.1 161,977 - GFF439 0.72 -0.8 162,109 - GFF439 0.78 +0.2 162,109 - GFF439 0.78 +1.6 162,192 + GFF439 0.82 -1.1 162,262 + GFF439 0.86 +0.2 162,266 + GFF439 0.86 +0.2 162,266 + GFF439 0.86 +0.5 162,266 + GFF439 0.86 +0.2 162,266 + GFF439 0.86 -0.5 162,267 - GFF439 0.86 +2.1 162,267 - GFF439 0.86 -0.5 162,278 - GFF439 0.87 +0.4 162,278 - GFF439 0.87 +0.3 162,674 - GFF440 0.56 +1.3 162,737 - -0.2 162,772 + -0.4 162,867 + -0.3 162,904 + -0.1 162,904 + -0.6 162,905 - -0.0 162,910 - -0.6 162,981 + +0.1 162,995 + -0.0 163,012 + +0.2 163,022 + +0.2 163,023 - -1.5 163,122 - -0.9 163,148 - +0.5 163,181 + GFF441 0.12 +0.2 163,270 + GFF441 0.27 +0.3 163,270 + GFF441 0.27 +1.3 163,283 - GFF441 0.29 -0.0 163,375 + GFF441 0.45 -0.8 163,376 - GFF441 0.46 -0.1 163,421 - GFF441 0.53 -1.5 163,488 - GFF441 0.65 -0.6 163,518 - GFF441 0.70 +1.3
Or see this region's nucleotide sequence