Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4272 and GFF4273 are separated by 14 nucleotides GFF4273 and GFF4274 are separated by 37 nucleotides GFF4274 and GFF4275 are separated by 267 nucleotides GFF4275 and GFF4276 are separated by 25 nucleotides
GFF4272 - SbmA protein, at 60,571 to 61,791
GFF4272
GFF4273 - Putative outer membrane lipoprotein, at 61,806 to 62,900
GFF4273
GFF4274 - Putative inner membrane protein, at 62,938 to 63,246
GFF4274
GFF4275 - Putative inner membrane protein, at 63,514 to 63,729
GFF4275
GFF4276 - D-alanine--D-alanine ligase A (EC 6.3.2.4), at 63,755 to 64,849
GFF4276
Position (kb) 61
62
63 Strain fitness (log2 ratio) -1
0
1
2
3 at 61.054 kb on + strand, within GFF4272 at 61.054 kb on + strand, within GFF4272 at 61.055 kb on - strand, within GFF4272 at 61.058 kb on + strand, within GFF4272 at 61.059 kb on - strand, within GFF4272 at 61.059 kb on - strand, within GFF4272 at 61.090 kb on + strand, within GFF4272 at 61.090 kb on + strand, within GFF4272 at 61.091 kb on - strand, within GFF4272 at 61.092 kb on + strand, within GFF4272 at 61.092 kb on + strand, within GFF4272 at 61.092 kb on + strand, within GFF4272 at 61.108 kb on + strand, within GFF4272 at 61.152 kb on + strand, within GFF4272 at 61.153 kb on - strand, within GFF4272 at 61.406 kb on - strand, within GFF4272 at 61.492 kb on - strand, within GFF4272 at 61.496 kb on - strand, within GFF4272 at 61.516 kb on + strand, within GFF4272 at 61.517 kb on - strand, within GFF4272 at 61.517 kb on - strand, within GFF4272 at 61.517 kb on - strand, within GFF4272 at 61.518 kb on + strand, within GFF4272 at 61.522 kb on + strand, within GFF4272 at 61.522 kb on + strand, within GFF4272 at 61.522 kb on + strand, within GFF4272 at 61.523 kb on - strand, within GFF4272 at 61.523 kb on - strand, within GFF4272 at 61.546 kb on + strand, within GFF4272 at 61.547 kb on - strand, within GFF4272 at 61.719 kb on + strand at 61.728 kb on - strand at 61.785 kb on + strand at 61.868 kb on + strand at 61.872 kb on - strand at 61.927 kb on + strand, within GFF4273 at 61.997 kb on + strand, within GFF4273 at 62.030 kb on + strand, within GFF4273 at 62.103 kb on - strand, within GFF4273 at 62.205 kb on + strand, within GFF4273 at 62.208 kb on - strand, within GFF4273 at 62.246 kb on + strand, within GFF4273 at 62.268 kb on - strand, within GFF4273 at 62.273 kb on + strand, within GFF4273 at 62.273 kb on + strand, within GFF4273 at 62.309 kb on + strand, within GFF4273 at 62.401 kb on + strand, within GFF4273 at 62.401 kb on + strand, within GFF4273 at 62.737 kb on - strand, within GFF4273 at 62.888 kb on + strand at 63.092 kb on + strand, within GFF4274 at 63.093 kb on - strand, within GFF4274 at 63.154 kb on + strand, within GFF4274 at 63.155 kb on - strand, within GFF4274 at 63.499 kb on + strand at 63.500 kb on - strand at 63.514 kb on - strand at 63.550 kb on + strand, within GFF4275 at 63.553 kb on + strand, within GFF4275 at 63.553 kb on + strand, within GFF4275 at 63.597 kb on - strand, within GFF4275 at 63.660 kb on + strand, within GFF4275 at 63.868 kb on - strand, within GFF4276 at 63.872 kb on - strand, within GFF4276
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 61,054 + GFF4272 0.40 +0.3 61,054 + GFF4272 0.40 +0.2 61,055 - GFF4272 0.40 -0.0 61,058 + GFF4272 0.40 +0.4 61,059 - GFF4272 0.40 +1.4 61,059 - GFF4272 0.40 +0.0 61,090 + GFF4272 0.43 +2.0 61,090 + GFF4272 0.43 -0.1 61,091 - GFF4272 0.43 +0.8 61,092 + GFF4272 0.43 +1.5 61,092 + GFF4272 0.43 +0.1 61,092 + GFF4272 0.43 +0.1 61,108 + GFF4272 0.44 +0.3 61,152 + GFF4272 0.48 -0.0 61,153 - GFF4272 0.48 -0.0 61,406 - GFF4272 0.68 -0.5 61,492 - GFF4272 0.75 +0.6 61,496 - GFF4272 0.76 -0.0 61,516 + GFF4272 0.77 +0.7 61,517 - GFF4272 0.77 -0.0 61,517 - GFF4272 0.77 +0.8 61,517 - GFF4272 0.77 -0.1 61,518 + GFF4272 0.78 -0.8 61,522 + GFF4272 0.78 +0.5 61,522 + GFF4272 0.78 -0.0 61,522 + GFF4272 0.78 -0.6 61,523 - GFF4272 0.78 +0.4 61,523 - GFF4272 0.78 +1.4 61,546 + GFF4272 0.80 +0.9 61,547 - GFF4272 0.80 +2.8 61,719 + +0.2 61,728 - +0.3 61,785 + -0.0 61,868 + +2.9 61,872 - -0.1 61,927 + GFF4273 0.11 +0.3 61,997 + GFF4273 0.17 +0.8 62,030 + GFF4273 0.20 +0.8 62,103 - GFF4273 0.27 +0.2 62,205 + GFF4273 0.36 -0.5 62,208 - GFF4273 0.37 +0.1 62,246 + GFF4273 0.40 +0.5 62,268 - GFF4273 0.42 +0.3 62,273 + GFF4273 0.43 +0.1 62,273 + GFF4273 0.43 -0.8 62,309 + GFF4273 0.46 +0.3 62,401 + GFF4273 0.54 -0.1 62,401 + GFF4273 0.54 +1.7 62,737 - GFF4273 0.85 -0.2 62,888 + -0.4 63,092 + GFF4274 0.50 +1.4 63,093 - GFF4274 0.50 +1.0 63,154 + GFF4274 0.70 +0.1 63,155 - GFF4274 0.70 +0.4 63,499 + +0.4 63,500 - +0.2 63,514 - +0.6 63,550 + GFF4275 0.17 +0.3 63,553 + GFF4275 0.18 -0.3 63,553 + GFF4275 0.18 +0.2 63,597 - GFF4275 0.38 +0.4 63,660 + GFF4275 0.68 +1.1 63,868 - GFF4276 0.10 -0.1 63,872 - GFF4276 0.11 +0.5
Or see this region's nucleotide sequence