Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4186 and GFF4187 are separated by 11 nucleotides GFF4187 and GFF4188 overlap by 4 nucleotides
GFF4186 - IMP cyclohydrolase (EC 3.5.4.10) / Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3), at 534 to 2,123
GFF4186
GFF4187 - Phosphoribosylamine--glycine ligase (EC 6.3.4.13), at 2,135 to 3,424
GFF4187
GFF4188 - Response regulator of zinc sigma-54-dependent two-component system, at 3,421 to 4,746
GFF4188
Position (kb)
2
3
4 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1.249 kb on - strand, within GFF4186 at 1.249 kb on - strand, within GFF4186 at 1.249 kb on - strand, within GFF4186 at 1.252 kb on + strand, within GFF4186 at 1.318 kb on - strand, within GFF4186 at 1.444 kb on - strand, within GFF4186 at 1.536 kb on - strand, within GFF4186 at 1.748 kb on + strand, within GFF4186 at 1.833 kb on + strand, within GFF4186 at 1.985 kb on + strand at 2.143 kb on - strand at 2.290 kb on + strand, within GFF4187 at 2.926 kb on + strand, within GFF4187 at 2.927 kb on - strand, within GFF4187 at 2.930 kb on + strand, within GFF4187 at 2.931 kb on - strand, within GFF4187 at 3.036 kb on + strand, within GFF4187 at 3.036 kb on + strand, within GFF4187 at 3.036 kb on + strand, within GFF4187 at 3.037 kb on - strand, within GFF4187 at 3.130 kb on - strand, within GFF4187 at 3.286 kb on - strand, within GFF4187 at 3.457 kb on - strand at 3.564 kb on - strand, within GFF4188 at 3.621 kb on - strand, within GFF4188 at 3.693 kb on - strand, within GFF4188 at 3.781 kb on + strand, within GFF4188 at 3.849 kb on - strand, within GFF4188 at 3.852 kb on - strand, within GFF4188 at 3.935 kb on - strand, within GFF4188 at 3.946 kb on - strand, within GFF4188 at 4.144 kb on - strand, within GFF4188 at 4.144 kb on - strand, within GFF4188
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 1,249 - GFF4186 0.45 -1.2 1,249 - GFF4186 0.45 -0.4 1,249 - GFF4186 0.45 -0.9 1,252 + GFF4186 0.45 +0.3 1,318 - GFF4186 0.49 -0.4 1,444 - GFF4186 0.57 -0.3 1,536 - GFF4186 0.63 -0.9 1,748 + GFF4186 0.76 +0.4 1,833 + GFF4186 0.82 +0.2 1,985 + -0.2 2,143 - +0.8 2,290 + GFF4187 0.12 +0.3 2,926 + GFF4187 0.61 +1.0 2,927 - GFF4187 0.61 -0.4 2,930 + GFF4187 0.62 -0.3 2,931 - GFF4187 0.62 -0.8 3,036 + GFF4187 0.70 -0.4 3,036 + GFF4187 0.70 -1.8 3,036 + GFF4187 0.70 -0.8 3,037 - GFF4187 0.70 +0.2 3,130 - GFF4187 0.77 +0.5 3,286 - GFF4187 0.89 -1.5 3,457 - +1.1 3,564 - GFF4188 0.11 -1.4 3,621 - GFF4188 0.15 -0.7 3,693 - GFF4188 0.21 -0.0 3,781 + GFF4188 0.27 -0.6 3,849 - GFF4188 0.32 +0.8 3,852 - GFF4188 0.33 +1.6 3,935 - GFF4188 0.39 +2.1 3,946 - GFF4188 0.40 -0.4 4,144 - GFF4188 0.55 -0.3 4,144 - GFF4188 0.55 +2.0
Or see this region's nucleotide sequence