Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4088 and GFF4089 are separated by 0 nucleotides GFF4089 and GFF4090 are separated by 336 nucleotides GFF4090 and GFF4091 are separated by 110 nucleotides
GFF4088 - hypothetical protein, at 47,561 to 47,686
GFF4088
GFF4089 - hypothetical protein, at 47,687 to 47,818
GFF4089
GFF4090 - Periplasmic fimbrial chaperone protein, at 48,155 to 48,952
GFF4090
GFF4091 - FIG034929: Fimbriae usher protein SafC, at 49,063 to 51,486
GFF4091
Position (kb)
48
49 Strain fitness (log2 ratio)
-1
0
1
2 at 47.942 kb on + strand at 48.051 kb on - strand at 48.152 kb on - strand at 48.268 kb on + strand, within GFF4090 at 48.280 kb on - strand, within GFF4090 at 48.313 kb on + strand, within GFF4090 at 48.574 kb on + strand, within GFF4090 at 48.665 kb on - strand, within GFF4090 at 48.817 kb on + strand, within GFF4090 at 48.817 kb on + strand, within GFF4090 at 48.830 kb on + strand, within GFF4090 at 48.830 kb on + strand, within GFF4090 at 48.830 kb on + strand, within GFF4090 at 48.831 kb on - strand, within GFF4090 at 48.863 kb on - strand, within GFF4090 at 48.902 kb on - strand at 48.947 kb on - strand at 49.092 kb on + strand at 49.092 kb on + strand at 49.092 kb on + strand at 49.093 kb on - strand at 49.116 kb on - strand at 49.132 kb on + strand at 49.159 kb on - strand at 49.280 kb on - strand at 49.477 kb on + strand, within GFF4091 at 49.515 kb on + strand, within GFF4091 at 49.515 kb on + strand, within GFF4091 at 49.566 kb on + strand, within GFF4091 at 49.568 kb on + strand, within GFF4091 at 49.642 kb on + strand, within GFF4091 at 49.643 kb on - strand, within GFF4091 at 49.643 kb on - strand, within GFF4091 at 49.644 kb on + strand, within GFF4091 at 49.645 kb on - strand, within GFF4091 at 49.645 kb on - strand, within GFF4091 at 49.645 kb on - strand, within GFF4091 at 49.648 kb on + strand, within GFF4091 at 49.666 kb on + strand, within GFF4091 at 49.666 kb on + strand, within GFF4091 at 49.666 kb on + strand, within GFF4091 at 49.719 kb on - strand, within GFF4091 at 49.719 kb on - strand, within GFF4091 at 49.923 kb on + strand, within GFF4091 at 49.923 kb on + strand, within GFF4091
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 47,942 + -0.8 48,051 - -0.6 48,152 - +1.1 48,268 + GFF4090 0.14 -0.2 48,280 - GFF4090 0.16 +1.5 48,313 + GFF4090 0.20 +2.5 48,574 + GFF4090 0.53 +0.3 48,665 - GFF4090 0.64 +0.5 48,817 + GFF4090 0.83 -1.3 48,817 + GFF4090 0.83 -0.6 48,830 + GFF4090 0.85 -0.2 48,830 + GFF4090 0.85 -0.3 48,830 + GFF4090 0.85 -0.1 48,831 - GFF4090 0.85 -0.2 48,863 - GFF4090 0.89 +0.5 48,902 - +0.5 48,947 - -0.7 49,092 + +2.5 49,092 + -1.4 49,092 + -1.0 49,093 - +0.1 49,116 - +1.1 49,132 + +0.8 49,159 - -0.7 49,280 - +0.5 49,477 + GFF4091 0.17 +0.2 49,515 + GFF4091 0.19 -0.4 49,515 + GFF4091 0.19 +1.8 49,566 + GFF4091 0.21 -0.1 49,568 + GFF4091 0.21 -0.3 49,642 + GFF4091 0.24 +0.2 49,643 - GFF4091 0.24 -1.3 49,643 - GFF4091 0.24 -1.2 49,644 + GFF4091 0.24 +0.6 49,645 - GFF4091 0.24 -0.1 49,645 - GFF4091 0.24 -0.3 49,645 - GFF4091 0.24 -0.5 49,648 + GFF4091 0.24 -0.2 49,666 + GFF4091 0.25 -0.5 49,666 + GFF4091 0.25 -0.3 49,666 + GFF4091 0.25 -0.4 49,719 - GFF4091 0.27 -0.1 49,719 - GFF4091 0.27 -0.5 49,923 + GFF4091 0.35 -0.4 49,923 + GFF4091 0.35 -0.1
Or see this region's nucleotide sequence