Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4025 and GFF4026 are separated by 71 nucleotides GFF4026 and GFF4027 overlap by 1 nucleotides GFF4027 and GFF4028 are separated by 14 nucleotides GFF4028 and GFF4029 are separated by 256 nucleotides
GFF4025 - Cytoplasmic alpha-amylase (EC 3.2.1.1), at 291,702 to 293,186
GFF4025
GFF4026 - Flagellar biosynthesis protein FliT, at 293,258 to 293,626
GFF4026
GFF4027 - Flagellar biosynthesis protein FliS, at 293,626 to 294,033
GFF4027
GFF4028 - Flagellar hook-associated protein FliD, at 294,048 to 295,451
GFF4028
GFF4029 - Flagellar biosynthesis protein FliC, at 295,708 to 297,195
GFF4029
Position (kb) 294
295
296 Strain fitness (log2 ratio) -2
-1
0
1 at 293.356 kb on + strand, within GFF4026 at 293.356 kb on + strand, within GFF4026 at 293.357 kb on - strand, within GFF4026 at 293.588 kb on - strand, within GFF4026 at 293.683 kb on - strand, within GFF4027 at 293.769 kb on - strand, within GFF4027 at 294.063 kb on - strand at 294.090 kb on - strand at 294.450 kb on - strand, within GFF4028 at 294.459 kb on - strand, within GFF4028 at 295.059 kb on - strand, within GFF4028 at 295.167 kb on - strand, within GFF4028 at 295.167 kb on - strand, within GFF4028 at 295.167 kb on - strand, within GFF4028 at 295.167 kb on - strand, within GFF4028 at 295.169 kb on - strand, within GFF4028 at 295.645 kb on + strand at 295.688 kb on + strand at 295.722 kb on + strand at 296.395 kb on + strand, within GFF4029
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 293,356 + GFF4026 0.27 -2.4 293,356 + GFF4026 0.27 -1.2 293,357 - GFF4026 0.27 +0.8 293,588 - GFF4026 0.89 +0.1 293,683 - GFF4027 0.14 -0.7 293,769 - GFF4027 0.35 -0.3 294,063 - -0.8 294,090 - -0.1 294,450 - GFF4028 0.29 +0.7 294,459 - GFF4028 0.29 -1.1 295,059 - GFF4028 0.72 -0.4 295,167 - GFF4028 0.80 -0.7 295,167 - GFF4028 0.80 +0.2 295,167 - GFF4028 0.80 -0.6 295,167 - GFF4028 0.80 -0.8 295,169 - GFF4028 0.80 -0.3 295,645 + +0.5 295,688 + +1.2 295,722 + +0.3 296,395 + GFF4029 0.46 -0.5
Or see this region's nucleotide sequence