Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3994 and GFF3995 are separated by 91 nucleotides GFF3995 and GFF3996 are separated by 73 nucleotides GFF3996 and GFF3997 overlap by 20 nucleotides GFF3997 and GFF3998 overlap by 12 nucleotides
GFF3994 - FIG00638388: hypothetical protein, at 267,967 to 268,233
GFF3994
GFF3995 - metal-dependent phosphohydrolase, at 268,325 to 269,020
GFF3995
GFF3996 - DNA-cytosine methyltransferase (EC 2.1.1.37), at 269,094 to 270,524
GFF3996
GFF3997 - Very-short-patch mismatch repair endonuclease (G-T specific), at 270,505 to 270,975
GFF3997
GFF3998 - Permease of the drug/metabolite transporter (DMT) superfamily, at 270,964 to 271,884
GFF3998
Position (kb) 269
270
271 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 268.231 kb on + strand at 268.231 kb on + strand at 268.243 kb on + strand at 268.243 kb on + strand at 268.244 kb on - strand at 268.309 kb on + strand at 268.311 kb on + strand at 268.491 kb on - strand, within GFF3995 at 268.645 kb on + strand, within GFF3995 at 268.776 kb on + strand, within GFF3995 at 268.934 kb on + strand, within GFF3995 at 268.934 kb on + strand, within GFF3995 at 268.934 kb on + strand, within GFF3995 at 269.054 kb on - strand at 269.059 kb on - strand at 269.094 kb on - strand at 269.358 kb on + strand, within GFF3996 at 269.456 kb on + strand, within GFF3996 at 269.457 kb on - strand, within GFF3996 at 269.457 kb on - strand, within GFF3996 at 269.475 kb on + strand, within GFF3996 at 269.478 kb on - strand, within GFF3996 at 269.478 kb on - strand, within GFF3996 at 270.069 kb on + strand, within GFF3996 at 270.101 kb on - strand, within GFF3996 at 270.148 kb on - strand, within GFF3996 at 270.159 kb on + strand, within GFF3996 at 270.205 kb on - strand, within GFF3996 at 270.673 kb on + strand, within GFF3997 at 270.724 kb on + strand, within GFF3997 at 270.725 kb on - strand, within GFF3997 at 270.725 kb on - strand, within GFF3997 at 270.805 kb on - strand, within GFF3997 at 270.965 kb on + strand at 271.194 kb on + strand, within GFF3998 at 271.194 kb on + strand, within GFF3998 at 271.195 kb on - strand, within GFF3998 at 271.195 kb on - strand, within GFF3998 at 271.195 kb on - strand, within GFF3998 at 271.216 kb on - strand, within GFF3998 at 271.414 kb on + strand, within GFF3998 at 271.451 kb on - strand, within GFF3998 at 271.451 kb on - strand, within GFF3998 at 271.451 kb on - strand, within GFF3998
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 268,231 + +1.3 268,231 + +0.0 268,243 + +0.1 268,243 + -0.1 268,244 - +1.2 268,309 + -0.1 268,311 + +1.8 268,491 - GFF3995 0.24 +1.7 268,645 + GFF3995 0.46 +0.2 268,776 + GFF3995 0.65 +0.7 268,934 + GFF3995 0.88 +0.4 268,934 + GFF3995 0.88 -0.2 268,934 + GFF3995 0.88 -0.7 269,054 - -0.5 269,059 - -0.3 269,094 - +0.8 269,358 + GFF3996 0.18 +1.1 269,456 + GFF3996 0.25 +0.9 269,457 - GFF3996 0.25 +0.6 269,457 - GFF3996 0.25 +0.3 269,475 + GFF3996 0.27 -0.5 269,478 - GFF3996 0.27 -0.5 269,478 - GFF3996 0.27 +0.6 270,069 + GFF3996 0.68 +3.7 270,101 - GFF3996 0.70 -1.3 270,148 - GFF3996 0.74 +2.9 270,159 + GFF3996 0.74 +0.6 270,205 - GFF3996 0.78 +4.3 270,673 + GFF3997 0.36 +0.1 270,724 + GFF3997 0.46 +0.0 270,725 - GFF3997 0.47 +0.7 270,725 - GFF3997 0.47 +3.0 270,805 - GFF3997 0.64 +0.0 270,965 + +0.6 271,194 + GFF3998 0.25 +0.0 271,194 + GFF3998 0.25 +0.7 271,195 - GFF3998 0.25 +0.4 271,195 - GFF3998 0.25 -0.2 271,195 - GFF3998 0.25 -0.2 271,216 - GFF3998 0.27 +1.5 271,414 + GFF3998 0.49 +1.5 271,451 - GFF3998 0.53 +0.0 271,451 - GFF3998 0.53 -0.7 271,451 - GFF3998 0.53 +1.2
Or see this region's nucleotide sequence