Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3955 and GFF3956 overlap by 17 nucleotides GFF3956 and GFF3957 overlap by 20 nucleotides GFF3957 and GFF3958 overlap by 1 nucleotides GFF3958 and GFF3959 overlap by 4 nucleotides
GFF3955 - Cobalt-precorrin-6y C15-methyltransferase [decarboxylating] (EC 2.1.1.-), at 235,758 to 236,336
GFF3955
GFF3956 - Cobalt-precorrin-4 C11-methyltransferase (EC 2.1.1.133), at 236,320 to 237,093
GFF3956
GFF3957 - Cobalamin biosynthesis protein CbiG, at 237,074 to 238,129
GFF3957
GFF3958 - Cobalt-precorrin-3b C17-methyltransferase, at 238,129 to 238,854
GFF3958
GFF3959 - Cobalt-precorrin-6x reductase (EC 1.3.1.54), at 238,851 to 239,642
GFF3959
Position (kb) 237
238
239 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 236.266 kb on - strand, within GFF3955 at 236.349 kb on - strand at 236.394 kb on + strand at 236.427 kb on - strand, within GFF3956 at 236.430 kb on + strand, within GFF3956 at 236.434 kb on + strand, within GFF3956 at 236.470 kb on + strand, within GFF3956 at 236.610 kb on - strand, within GFF3956 at 236.643 kb on + strand, within GFF3956 at 236.658 kb on - strand, within GFF3956 at 236.658 kb on - strand, within GFF3956 at 236.712 kb on + strand, within GFF3956 at 236.713 kb on - strand, within GFF3956 at 236.743 kb on - strand, within GFF3956 at 236.763 kb on + strand, within GFF3956 at 236.828 kb on - strand, within GFF3956 at 236.976 kb on - strand, within GFF3956 at 236.976 kb on - strand, within GFF3956 at 237.176 kb on - strand at 237.176 kb on - strand at 237.250 kb on + strand, within GFF3957 at 237.269 kb on - strand, within GFF3957 at 237.505 kb on - strand, within GFF3957 at 237.518 kb on - strand, within GFF3957 at 237.650 kb on - strand, within GFF3957 at 237.911 kb on - strand, within GFF3957 at 238.108 kb on + strand at 238.108 kb on + strand at 238.108 kb on + strand at 238.108 kb on + strand at 238.430 kb on + strand, within GFF3958 at 238.443 kb on + strand, within GFF3958 at 238.563 kb on + strand, within GFF3958 at 238.563 kb on + strand, within GFF3958 at 238.603 kb on + strand, within GFF3958 at 238.766 kb on + strand, within GFF3958 at 238.770 kb on + strand, within GFF3958 at 238.807 kb on + strand at 238.808 kb on - strand at 238.843 kb on + strand at 238.935 kb on + strand, within GFF3959 at 238.936 kb on - strand, within GFF3959 at 238.985 kb on + strand, within GFF3959 at 239.065 kb on + strand, within GFF3959 at 239.065 kb on + strand, within GFF3959 at 239.066 kb on - strand, within GFF3959 at 239.102 kb on + strand, within GFF3959
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 236,266 - GFF3955 0.88 -0.5 236,349 - -0.1 236,394 + +1.8 236,427 - GFF3956 0.14 +0.9 236,430 + GFF3956 0.14 +0.6 236,434 + GFF3956 0.15 +1.1 236,470 + GFF3956 0.19 +0.0 236,610 - GFF3956 0.37 -0.6 236,643 + GFF3956 0.42 +0.0 236,658 - GFF3956 0.44 +1.2 236,658 - GFF3956 0.44 -0.9 236,712 + GFF3956 0.51 -0.4 236,713 - GFF3956 0.51 +1.0 236,743 - GFF3956 0.55 +2.8 236,763 + GFF3956 0.57 +0.6 236,828 - GFF3956 0.66 +4.3 236,976 - GFF3956 0.85 -0.8 236,976 - GFF3956 0.85 +1.9 237,176 - -1.1 237,176 - -2.3 237,250 + GFF3957 0.17 -2.0 237,269 - GFF3957 0.18 +0.5 237,505 - GFF3957 0.41 +3.5 237,518 - GFF3957 0.42 +0.5 237,650 - GFF3957 0.55 +5.1 237,911 - GFF3957 0.79 -0.5 238,108 + -0.6 238,108 + +0.1 238,108 + +0.3 238,108 + -0.1 238,430 + GFF3958 0.41 -0.5 238,443 + GFF3958 0.43 -2.3 238,563 + GFF3958 0.60 +0.1 238,563 + GFF3958 0.60 +0.5 238,603 + GFF3958 0.65 +3.5 238,766 + GFF3958 0.88 -0.4 238,770 + GFF3958 0.88 -0.3 238,807 + -0.9 238,808 - +0.0 238,843 + +0.1 238,935 + GFF3959 0.11 -0.8 238,936 - GFF3959 0.11 +0.0 238,985 + GFF3959 0.17 -0.4 239,065 + GFF3959 0.27 +2.0 239,065 + GFF3959 0.27 -0.3 239,066 - GFF3959 0.27 +0.5 239,102 + GFF3959 0.32 +0.8
Or see this region's nucleotide sequence