Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3857 and GFF3858 are separated by 133 nucleotides GFF3858 and GFF3859 overlap by 33 nucleotides GFF3859 and GFF3860 are separated by 78 nucleotides GFF3860 and GFF3861 are separated by 75 nucleotides
GFF3857 - Putative heat shock protein YegD, at 131,009 to 132,361
GFF3857
GFF3858 - DNA-3-methyladenine glycosylase II (EC 3.2.2.21), at 132,495 to 133,364
GFF3858
GFF3859 - FIG00509877: hypothetical protein, at 133,332 to 136,328
GFF3859
GFF3860 - FIG01045963: hypothetical protein, at 136,407 to 136,577
GFF3860
GFF3861 - Uridine kinase (EC 2.7.1.48) [C1], at 136,653 to 137,294
GFF3861
Position (kb) 133
134
135
136
137 Strain fitness (log2 ratio) -1
0
1
2
3 at 132.365 kb on - strand at 132.465 kb on - strand at 132.522 kb on + strand at 132.694 kb on + strand, within GFF3858 at 132.830 kb on + strand, within GFF3858 at 132.830 kb on + strand, within GFF3858 at 133.217 kb on + strand, within GFF3858 at 133.291 kb on - strand at 133.348 kb on + strand at 133.422 kb on - strand at 133.576 kb on + strand at 133.600 kb on - strand at 133.731 kb on + strand, within GFF3859 at 133.731 kb on + strand, within GFF3859 at 133.732 kb on - strand, within GFF3859 at 133.799 kb on - strand, within GFF3859 at 134.069 kb on + strand, within GFF3859 at 134.069 kb on + strand, within GFF3859 at 134.069 kb on + strand, within GFF3859 at 134.216 kb on + strand, within GFF3859 at 134.217 kb on - strand, within GFF3859 at 134.217 kb on - strand, within GFF3859 at 134.227 kb on - strand, within GFF3859 at 134.307 kb on - strand, within GFF3859 at 134.340 kb on - strand, within GFF3859 at 134.343 kb on - strand, within GFF3859 at 134.443 kb on - strand, within GFF3859 at 134.718 kb on - strand, within GFF3859 at 134.808 kb on - strand, within GFF3859 at 134.829 kb on - strand, within GFF3859 at 134.859 kb on + strand, within GFF3859 at 134.922 kb on - strand, within GFF3859 at 134.949 kb on - strand, within GFF3859 at 135.030 kb on - strand, within GFF3859 at 135.032 kb on + strand, within GFF3859 at 135.107 kb on - strand, within GFF3859 at 135.181 kb on + strand, within GFF3859 at 135.182 kb on - strand, within GFF3859 at 135.182 kb on - strand, within GFF3859 at 135.185 kb on - strand, within GFF3859 at 135.202 kb on + strand, within GFF3859 at 135.203 kb on - strand, within GFF3859 at 135.203 kb on - strand, within GFF3859 at 135.203 kb on - strand, within GFF3859 at 135.234 kb on + strand, within GFF3859 at 135.521 kb on + strand, within GFF3859 at 135.522 kb on - strand, within GFF3859 at 135.689 kb on + strand, within GFF3859 at 135.689 kb on + strand, within GFF3859 at 135.691 kb on + strand, within GFF3859 at 135.691 kb on + strand, within GFF3859 at 135.781 kb on + strand, within GFF3859 at 135.781 kb on + strand, within GFF3859 at 135.799 kb on - strand, within GFF3859 at 135.844 kb on - strand, within GFF3859 at 135.897 kb on + strand, within GFF3859 at 135.979 kb on + strand, within GFF3859 at 136.035 kb on + strand at 136.218 kb on + strand at 136.276 kb on - strand at 136.324 kb on - strand at 136.388 kb on - strand at 136.388 kb on - strand at 136.393 kb on - strand at 136.393 kb on - strand at 136.396 kb on - strand at 136.430 kb on + strand, within GFF3860 at 136.586 kb on + strand at 136.623 kb on + strand at 136.623 kb on + strand at 136.623 kb on + strand at 136.624 kb on - strand at 136.797 kb on + strand, within GFF3861 at 136.800 kb on + strand, within GFF3861 at 136.801 kb on - strand, within GFF3861 at 136.848 kb on + strand, within GFF3861 at 136.849 kb on - strand, within GFF3861 at 136.935 kb on + strand, within GFF3861 at 136.935 kb on + strand, within GFF3861 at 136.936 kb on - strand, within GFF3861 at 136.941 kb on + strand, within GFF3861 at 136.942 kb on - strand, within GFF3861 at 136.997 kb on + strand, within GFF3861 at 137.009 kb on + strand, within GFF3861 at 137.209 kb on - strand, within GFF3861 at 137.217 kb on - strand, within GFF3861
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 132,365 - -1.2 132,465 - +1.1 132,522 + +1.2 132,694 + GFF3858 0.23 +0.3 132,830 + GFF3858 0.39 -0.3 132,830 + GFF3858 0.39 +0.2 133,217 + GFF3858 0.83 -0.7 133,291 - +0.8 133,348 + -0.3 133,422 - -0.3 133,576 + +1.2 133,600 - -0.8 133,731 + GFF3859 0.13 +0.2 133,731 + GFF3859 0.13 +2.0 133,732 - GFF3859 0.13 +0.4 133,799 - GFF3859 0.16 +0.2 134,069 + GFF3859 0.25 -0.1 134,069 + GFF3859 0.25 -0.5 134,069 + GFF3859 0.25 +0.1 134,216 + GFF3859 0.29 +3.2 134,217 - GFF3859 0.30 +0.2 134,217 - GFF3859 0.30 -1.0 134,227 - GFF3859 0.30 +0.2 134,307 - GFF3859 0.33 -0.4 134,340 - GFF3859 0.34 +0.2 134,343 - GFF3859 0.34 +0.0 134,443 - GFF3859 0.37 -0.3 134,718 - GFF3859 0.46 +1.5 134,808 - GFF3859 0.49 -0.9 134,829 - GFF3859 0.50 -0.5 134,859 + GFF3859 0.51 -1.4 134,922 - GFF3859 0.53 -0.3 134,949 - GFF3859 0.54 -0.9 135,030 - GFF3859 0.57 +0.5 135,032 + GFF3859 0.57 +0.0 135,107 - GFF3859 0.59 +0.2 135,181 + GFF3859 0.62 -0.8 135,182 - GFF3859 0.62 +0.2 135,182 - GFF3859 0.62 -0.5 135,185 - GFF3859 0.62 +0.2 135,202 + GFF3859 0.62 +0.5 135,203 - GFF3859 0.62 -0.1 135,203 - GFF3859 0.62 +1.5 135,203 - GFF3859 0.62 -0.7 135,234 + GFF3859 0.63 +1.3 135,521 + GFF3859 0.73 -0.0 135,522 - GFF3859 0.73 +1.4 135,689 + GFF3859 0.79 -0.2 135,689 + GFF3859 0.79 -0.7 135,691 + GFF3859 0.79 -0.3 135,691 + GFF3859 0.79 +0.3 135,781 + GFF3859 0.82 +3.4 135,781 + GFF3859 0.82 +0.2 135,799 - GFF3859 0.82 +1.3 135,844 - GFF3859 0.84 -0.6 135,897 + GFF3859 0.86 -0.5 135,979 + GFF3859 0.88 +0.2 136,035 + -0.0 136,218 + +2.8 136,276 - -0.4 136,324 - +1.2 136,388 - -1.0 136,388 - -0.8 136,393 - -0.1 136,393 - -1.3 136,396 - +2.1 136,430 + GFF3860 0.13 +1.1 136,586 + +0.6 136,623 + +0.6 136,623 + -0.9 136,623 + +0.3 136,624 - -0.1 136,797 + GFF3861 0.22 -0.5 136,800 + GFF3861 0.23 -1.0 136,801 - GFF3861 0.23 -0.4 136,848 + GFF3861 0.30 -0.5 136,849 - GFF3861 0.31 -0.1 136,935 + GFF3861 0.44 -0.5 136,935 + GFF3861 0.44 -1.1 136,936 - GFF3861 0.44 -0.8 136,941 + GFF3861 0.45 +0.0 136,942 - GFF3861 0.45 +0.0 136,997 + GFF3861 0.54 -0.2 137,009 + GFF3861 0.55 -0.4 137,209 - GFF3861 0.87 +0.2 137,217 - GFF3861 0.88 -0.8
Or see this region's nucleotide sequence