Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3766 and GFF3767 are separated by 1 nucleotides GFF3767 and GFF3768 are separated by 64 nucleotides GFF3768 and GFF3769 are separated by 9 nucleotides GFF3769 and GFF3770 are separated by 84 nucleotides GFF3770 and GFF3771 are separated by 20 nucleotides
GFF3766 - hypothetical protein, at 35,093 to 35,215
GFF3766
GFF3767 - Putative inner membrane protein, at 35,217 to 35,882
GFF3767
GFF3768 - Sugar efflux transporter B, at 35,947 to 37,128
GFF3768
GFF3769 - hypothetical protein, at 37,138 to 37,260
GFF3769
GFF3770 - hypothetical protein, at 37,345 to 37,476
GFF3770
GFF3771 - Fructose-specific phosphocarrier protein HPr (EC 2.7.1.69) / PTS system, fructose-specific IIA component (EC 2.7.1.69), at 37,497 to 38,627
GFF3771
Position (kb) 35
36
37
38 Strain fitness (log2 ratio) -2
-1
0
1
2 at 35.304 kb on - strand, within GFF3767 at 35.755 kb on - strand, within GFF3767 at 35.825 kb on + strand at 36.093 kb on + strand, within GFF3768 at 36.176 kb on + strand, within GFF3768 at 36.176 kb on + strand, within GFF3768 at 36.243 kb on - strand, within GFF3768 at 36.414 kb on + strand, within GFF3768 at 36.425 kb on + strand, within GFF3768 at 36.425 kb on + strand, within GFF3768 at 36.425 kb on + strand, within GFF3768 at 36.436 kb on - strand, within GFF3768 at 36.436 kb on - strand, within GFF3768 at 36.603 kb on - strand, within GFF3768 at 36.618 kb on + strand, within GFF3768 at 36.815 kb on + strand, within GFF3768 at 36.815 kb on + strand, within GFF3768 at 37.098 kb on + strand at 37.134 kb on + strand at 37.241 kb on - strand, within GFF3769 at 37.298 kb on + strand at 37.622 kb on + strand, within GFF3771 at 37.777 kb on - strand, within GFF3771 at 37.790 kb on + strand, within GFF3771
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 35,304 - GFF3767 0.13 -0.4 35,755 - GFF3767 0.81 +0.3 35,825 + +0.1 36,093 + GFF3768 0.12 +0.3 36,176 + GFF3768 0.19 -0.1 36,176 + GFF3768 0.19 -1.5 36,243 - GFF3768 0.25 +0.5 36,414 + GFF3768 0.40 -0.7 36,425 + GFF3768 0.40 +0.2 36,425 + GFF3768 0.40 +0.3 36,425 + GFF3768 0.40 +0.2 36,436 - GFF3768 0.41 +0.2 36,436 - GFF3768 0.41 +2.6 36,603 - GFF3768 0.55 +0.4 36,618 + GFF3768 0.57 -0.6 36,815 + GFF3768 0.73 -0.9 36,815 + GFF3768 0.73 +1.6 37,098 + +0.6 37,134 + +0.0 37,241 - GFF3769 0.84 -0.2 37,298 + +0.5 37,622 + GFF3771 0.11 +0.0 37,777 - GFF3771 0.25 -2.7 37,790 + GFF3771 0.26 -0.2
Or see this region's nucleotide sequence