Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3754 and GFF3755 are separated by 390 nucleotides GFF3755 and GFF3756 are separated by 3 nucleotides GFF3756 and GFF3757 are separated by 1 nucleotides
GFF3754 - MFS family multidrug transport protein, bicyclomycin resistance protein, at 21,178 to 22,368
GFF3754
GFF3755 - FIG00896318: hypothetical protein, at 22,759 to 23,103
GFF3755
GFF3756 - Putative ABC transporter ATP-binding protein, at 23,107 to 24,696
GFF3756
GFF3757 - Oligopeptide transport system permease protein OppC (TC 3.A.1.5.1), at 24,698 to 25,723
GFF3757
Position (kb)
23
24
25 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 22.471 kb on - strand at 22.471 kb on - strand at 22.471 kb on - strand at 22.533 kb on + strand at 22.555 kb on + strand at 22.590 kb on + strand at 22.624 kb on - strand at 22.769 kb on + strand at 22.890 kb on + strand, within GFF3755 at 22.936 kb on - strand, within GFF3755 at 23.091 kb on + strand at 23.091 kb on + strand at 23.101 kb on + strand at 23.134 kb on - strand at 23.136 kb on - strand at 23.234 kb on - strand at 23.251 kb on - strand at 23.261 kb on + strand at 23.646 kb on + strand, within GFF3756 at 23.646 kb on + strand, within GFF3756 at 23.785 kb on - strand, within GFF3756 at 23.803 kb on - strand, within GFF3756 at 23.942 kb on + strand, within GFF3756 at 23.942 kb on + strand, within GFF3756 at 24.061 kb on - strand, within GFF3756 at 24.109 kb on + strand, within GFF3756 at 24.166 kb on + strand, within GFF3756 at 24.659 kb on - strand at 24.699 kb on + strand at 24.967 kb on + strand, within GFF3757 at 24.968 kb on - strand, within GFF3757 at 25.024 kb on - strand, within GFF3757 at 25.408 kb on + strand, within GFF3757 at 25.415 kb on - strand, within GFF3757 at 25.424 kb on - strand, within GFF3757 at 25.598 kb on - strand, within GFF3757 at 25.650 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 22,471 - +3.1 22,471 - +0.0 22,471 - -0.6 22,533 + +1.1 22,555 + -0.5 22,590 + -0.0 22,624 - -0.3 22,769 + -1.2 22,890 + GFF3755 0.38 -0.2 22,936 - GFF3755 0.51 -0.4 23,091 + -0.2 23,091 + -0.1 23,101 + +1.7 23,134 - +0.1 23,136 - +0.9 23,234 - -0.5 23,251 - -0.0 23,261 + -0.1 23,646 + GFF3756 0.34 +0.1 23,646 + GFF3756 0.34 -1.5 23,785 - GFF3756 0.43 -2.1 23,803 - GFF3756 0.44 -1.5 23,942 + GFF3756 0.53 -1.0 23,942 + GFF3756 0.53 +0.1 24,061 - GFF3756 0.60 +0.2 24,109 + GFF3756 0.63 -0.7 24,166 + GFF3756 0.67 -0.5 24,659 - -1.0 24,699 + -0.7 24,967 + GFF3757 0.26 +0.6 24,968 - GFF3757 0.26 -1.1 25,024 - GFF3757 0.32 -0.7 25,408 + GFF3757 0.69 +0.4 25,415 - GFF3757 0.70 -0.0 25,424 - GFF3757 0.71 +3.2 25,598 - GFF3757 0.88 -0.8 25,650 - +1.0
Or see this region's nucleotide sequence