Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3632 and GFF3633 are separated by 47 nucleotides GFF3633 and GFF3634 are separated by 271 nucleotides GFF3634 and GFF3635 are separated by 93 nucleotides GFF3635 and GFF3636 are separated by 58 nucleotides
GFF3632 - Transcriptional regulator SlyA, at 382,047 to 382,373
GFF3632
GFF3633 - Outer membrane lipoprotein pcp precursor, at 382,421 to 382,888
GFF3633
GFF3634 - Anhydro-N-acetylmuramic acid kinase (EC 2.7.1.-), at 383,160 to 384,281
GFF3634
GFF3635 - Membrane-bound lysozyme inhibitor of c-type lysozyme, at 384,375 to 384,698
GFF3635
GFF3636 - 'Pyridoxamine 5'-phosphate oxidase (EC 1.4.3.5)' transl_table=11, at 384,757 to 385,413
GFF3636
Position (kb) 383
384
385 Strain fitness (log2 ratio) -1
0
1
2
3 at 382.208 kb on + strand, within GFF3632 at 382.263 kb on - strand, within GFF3632 at 382.386 kb on + strand at 382.411 kb on - strand at 382.411 kb on - strand at 382.493 kb on - strand, within GFF3633 at 382.757 kb on - strand, within GFF3633 at 382.765 kb on - strand, within GFF3633 at 382.765 kb on - strand, within GFF3633 at 382.875 kb on - strand at 382.890 kb on + strand at 382.932 kb on + strand at 382.935 kb on - strand at 382.939 kb on - strand at 382.943 kb on + strand at 382.945 kb on + strand at 383.010 kb on + strand at 383.105 kb on + strand at 383.106 kb on - strand at 383.117 kb on + strand at 383.146 kb on + strand at 383.309 kb on + strand, within GFF3634 at 383.331 kb on - strand, within GFF3634 at 383.493 kb on - strand, within GFF3634 at 383.513 kb on + strand, within GFF3634 at 383.648 kb on + strand, within GFF3634 at 383.648 kb on + strand, within GFF3634 at 383.735 kb on + strand, within GFF3634 at 383.736 kb on - strand, within GFF3634 at 383.736 kb on - strand, within GFF3634 at 383.901 kb on + strand, within GFF3634 at 383.901 kb on + strand, within GFF3634 at 383.902 kb on - strand, within GFF3634 at 383.902 kb on - strand, within GFF3634 at 383.911 kb on + strand, within GFF3634 at 383.956 kb on + strand, within GFF3634 at 384.098 kb on + strand, within GFF3634 at 384.119 kb on + strand, within GFF3634 at 384.120 kb on - strand, within GFF3634 at 384.143 kb on + strand, within GFF3634 at 384.314 kb on + strand at 384.462 kb on - strand, within GFF3635 at 384.591 kb on + strand, within GFF3635 at 384.594 kb on - strand, within GFF3635 at 384.666 kb on - strand at 384.668 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 382,208 + GFF3632 0.49 -0.2 382,263 - GFF3632 0.66 -1.4 382,386 + -0.8 382,411 - -0.5 382,411 - -0.3 382,493 - GFF3633 0.15 +2.8 382,757 - GFF3633 0.72 +1.4 382,765 - GFF3633 0.74 -0.3 382,765 - GFF3633 0.74 +1.6 382,875 - -0.3 382,890 + +1.1 382,932 + +0.4 382,935 - +0.1 382,939 - +0.7 382,943 + +0.7 382,945 + +1.4 383,010 + +0.0 383,105 + +0.6 383,106 - +0.0 383,117 + -0.5 383,146 + -0.1 383,309 + GFF3634 0.13 +1.6 383,331 - GFF3634 0.15 +0.6 383,493 - GFF3634 0.30 +1.1 383,513 + GFF3634 0.31 -0.2 383,648 + GFF3634 0.43 +1.1 383,648 + GFF3634 0.43 +0.6 383,735 + GFF3634 0.51 +0.0 383,736 - GFF3634 0.51 -0.5 383,736 - GFF3634 0.51 +0.1 383,901 + GFF3634 0.66 -0.0 383,901 + GFF3634 0.66 -0.4 383,902 - GFF3634 0.66 -0.4 383,902 - GFF3634 0.66 -0.5 383,911 + GFF3634 0.67 +3.6 383,956 + GFF3634 0.71 +0.3 384,098 + GFF3634 0.84 +0.5 384,119 + GFF3634 0.85 +0.3 384,120 - GFF3634 0.86 -0.1 384,143 + GFF3634 0.88 -0.8 384,314 + +0.1 384,462 - GFF3635 0.27 +0.4 384,591 + GFF3635 0.67 +0.5 384,594 - GFF3635 0.68 -0.5 384,666 - -1.1 384,668 + -0.8
Or see this region's nucleotide sequence