Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3630 and GFF3631 are separated by 2 nucleotides GFF3631 and GFF3632 are separated by 353 nucleotides GFF3632 and GFF3633 are separated by 47 nucleotides GFF3633 and GFF3634 are separated by 271 nucleotides
GFF3630 - Putative membrane protein, at 380,639 to 381,499
GFF3630
GFF3631 - hypothetical protein, at 381,502 to 381,693
GFF3631
GFF3632 - Transcriptional regulator SlyA, at 382,047 to 382,373
GFF3632
GFF3633 - Outer membrane lipoprotein pcp precursor, at 382,421 to 382,888
GFF3633
GFF3634 - Anhydro-N-acetylmuramic acid kinase (EC 2.7.1.-), at 383,160 to 384,281
GFF3634
Position (kb) 382
383 Strain fitness (log2 ratio) -1
0
1
2
3 at 381.095 kb on + strand, within GFF3630 at 381.095 kb on + strand, within GFF3630 at 381.116 kb on - strand, within GFF3630 at 381.413 kb on - strand, within GFF3630 at 381.414 kb on + strand at 381.414 kb on + strand at 381.415 kb on - strand at 381.535 kb on + strand, within GFF3631 at 381.566 kb on - strand, within GFF3631 at 381.729 kb on + strand at 381.795 kb on + strand at 381.816 kb on - strand at 381.816 kb on - strand at 382.129 kb on + strand, within GFF3632 at 382.208 kb on + strand, within GFF3632 at 382.263 kb on - strand, within GFF3632 at 382.386 kb on + strand at 382.411 kb on - strand at 382.411 kb on - strand at 382.493 kb on - strand, within GFF3633 at 382.757 kb on - strand, within GFF3633 at 382.765 kb on - strand, within GFF3633 at 382.765 kb on - strand, within GFF3633 at 382.875 kb on - strand at 382.890 kb on + strand at 382.932 kb on + strand at 382.935 kb on - strand at 382.939 kb on - strand at 382.943 kb on + strand at 382.945 kb on + strand at 383.010 kb on + strand at 383.105 kb on + strand at 383.106 kb on - strand at 383.117 kb on + strand at 383.146 kb on + strand at 383.309 kb on + strand, within GFF3634 at 383.331 kb on - strand, within GFF3634
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 381,095 + GFF3630 0.53 +0.3 381,095 + GFF3630 0.53 -1.1 381,116 - GFF3630 0.55 -0.1 381,413 - GFF3630 0.90 +0.4 381,414 + -0.8 381,414 + +2.5 381,415 - -0.0 381,535 + GFF3631 0.17 -0.2 381,566 - GFF3631 0.33 +0.3 381,729 + -0.5 381,795 + +0.8 381,816 - +1.6 381,816 - -0.1 382,129 + GFF3632 0.25 -0.3 382,208 + GFF3632 0.49 -0.2 382,263 - GFF3632 0.66 -1.4 382,386 + -0.8 382,411 - -0.5 382,411 - -0.3 382,493 - GFF3633 0.15 +2.8 382,757 - GFF3633 0.72 +1.4 382,765 - GFF3633 0.74 -0.3 382,765 - GFF3633 0.74 +1.6 382,875 - -0.3 382,890 + +1.1 382,932 + +0.4 382,935 - +0.1 382,939 - +0.7 382,943 + +0.7 382,945 + +1.4 383,010 + +0.0 383,105 + +0.6 383,106 - +0.0 383,117 + -0.5 383,146 + -0.1 383,309 + GFF3634 0.13 +1.6 383,331 - GFF3634 0.15 +0.6
Or see this region's nucleotide sequence