Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3384 and GFF3385 are separated by 84 nucleotides GFF3385 and GFF3386 are separated by 323 nucleotides GFF3386 and GFF3387 are separated by 2 nucleotides
GFF3384 - PTS system, glucose-specific IIB component (EC 2.7.1.69) / PTS system, glucose-specific IIC component (EC 2.7.1.69), at 148,421 to 149,854
GFF3384
GFF3385 - Putative OMR family iron-siderophore receptor precursor, at 149,939 to 152,113
GFF3385
GFF3386 - YcfF/hinT protein: a purine nucleoside phosphoramidase, at 152,437 to 152,814
GFF3386
GFF3387 - YcfL protein: an outer membrane lipoprotein that is part of a salvage cluster, at 152,817 to 153,191
GFF3387
Position (kb) 149
150
151
152
153 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 149.165 kb on - strand, within GFF3384 at 149.360 kb on - strand, within GFF3384 at 149.399 kb on - strand, within GFF3384 at 149.399 kb on - strand, within GFF3384 at 149.423 kb on - strand, within GFF3384 at 149.669 kb on - strand, within GFF3384 at 149.846 kb on - strand at 149.852 kb on + strand at 150.102 kb on + strand at 150.146 kb on - strand at 150.254 kb on - strand, within GFF3385 at 150.328 kb on + strand, within GFF3385 at 150.442 kb on + strand, within GFF3385 at 150.520 kb on - strand, within GFF3385 at 150.523 kb on + strand, within GFF3385 at 150.592 kb on + strand, within GFF3385 at 150.592 kb on + strand, within GFF3385 at 150.593 kb on - strand, within GFF3385 at 150.630 kb on + strand, within GFF3385 at 150.703 kb on + strand, within GFF3385 at 150.703 kb on + strand, within GFF3385 at 150.704 kb on - strand, within GFF3385 at 150.763 kb on - strand, within GFF3385 at 150.784 kb on + strand, within GFF3385 at 150.910 kb on + strand, within GFF3385 at 150.913 kb on + strand, within GFF3385 at 150.915 kb on + strand, within GFF3385 at 150.916 kb on - strand, within GFF3385 at 150.960 kb on + strand, within GFF3385 at 150.983 kb on + strand, within GFF3385 at 150.983 kb on + strand, within GFF3385 at 150.983 kb on + strand, within GFF3385 at 150.993 kb on - strand, within GFF3385 at 150.993 kb on - strand, within GFF3385 at 151.098 kb on + strand, within GFF3385 at 151.098 kb on + strand, within GFF3385 at 151.098 kb on + strand, within GFF3385 at 151.098 kb on + strand, within GFF3385 at 151.099 kb on - strand, within GFF3385 at 151.099 kb on - strand, within GFF3385 at 151.200 kb on - strand, within GFF3385 at 151.214 kb on - strand, within GFF3385 at 151.216 kb on - strand, within GFF3385 at 151.238 kb on - strand, within GFF3385 at 151.251 kb on + strand, within GFF3385 at 151.251 kb on + strand, within GFF3385 at 151.256 kb on - strand, within GFF3385 at 151.503 kb on + strand, within GFF3385 at 151.514 kb on - strand, within GFF3385 at 151.527 kb on + strand, within GFF3385 at 151.543 kb on + strand, within GFF3385 at 151.585 kb on + strand, within GFF3385 at 151.585 kb on + strand, within GFF3385 at 151.717 kb on + strand, within GFF3385 at 151.731 kb on + strand, within GFF3385 at 151.732 kb on - strand, within GFF3385 at 152.027 kb on - strand at 152.047 kb on + strand at 152.052 kb on - strand at 152.246 kb on + strand at 152.324 kb on - strand at 152.389 kb on + strand at 152.412 kb on + strand at 152.571 kb on + strand, within GFF3386 at 152.718 kb on + strand, within GFF3386 at 152.746 kb on + strand, within GFF3386 at 152.751 kb on + strand, within GFF3386 at 152.812 kb on + strand at 152.813 kb on - strand at 152.816 kb on + strand at 152.816 kb on + strand at 152.817 kb on - strand at 153.048 kb on + strand, within GFF3387
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 149,165 - GFF3384 0.52 -1.1 149,360 - GFF3384 0.65 -0.1 149,399 - GFF3384 0.68 -1.9 149,399 - GFF3384 0.68 -0.1 149,423 - GFF3384 0.70 -0.8 149,669 - GFF3384 0.87 +0.5 149,846 - -0.1 149,852 + +0.5 150,102 + +1.0 150,146 - -0.1 150,254 - GFF3385 0.14 -0.3 150,328 + GFF3385 0.18 -0.9 150,442 + GFF3385 0.23 -0.2 150,520 - GFF3385 0.27 -0.2 150,523 + GFF3385 0.27 +0.1 150,592 + GFF3385 0.30 +0.9 150,592 + GFF3385 0.30 +1.5 150,593 - GFF3385 0.30 -0.2 150,630 + GFF3385 0.32 +0.1 150,703 + GFF3385 0.35 -0.1 150,703 + GFF3385 0.35 +0.3 150,704 - GFF3385 0.35 +0.1 150,763 - GFF3385 0.38 +1.2 150,784 + GFF3385 0.39 -0.5 150,910 + GFF3385 0.45 -0.5 150,913 + GFF3385 0.45 -0.3 150,915 + GFF3385 0.45 -0.1 150,916 - GFF3385 0.45 -0.2 150,960 + GFF3385 0.47 -0.1 150,983 + GFF3385 0.48 +0.8 150,983 + GFF3385 0.48 -0.0 150,983 + GFF3385 0.48 -0.4 150,993 - GFF3385 0.48 -0.3 150,993 - GFF3385 0.48 +1.9 151,098 + GFF3385 0.53 -0.1 151,098 + GFF3385 0.53 -0.3 151,098 + GFF3385 0.53 -0.5 151,098 + GFF3385 0.53 -0.6 151,099 - GFF3385 0.53 -0.4 151,099 - GFF3385 0.53 +0.7 151,200 - GFF3385 0.58 +0.0 151,214 - GFF3385 0.59 +1.2 151,216 - GFF3385 0.59 +0.7 151,238 - GFF3385 0.60 -0.6 151,251 + GFF3385 0.60 -1.3 151,251 + GFF3385 0.60 +1.9 151,256 - GFF3385 0.61 -0.1 151,503 + GFF3385 0.72 +0.0 151,514 - GFF3385 0.72 -0.2 151,527 + GFF3385 0.73 +0.2 151,543 + GFF3385 0.74 -0.3 151,585 + GFF3385 0.76 -0.1 151,585 + GFF3385 0.76 +0.5 151,717 + GFF3385 0.82 -0.5 151,731 + GFF3385 0.82 +0.4 151,732 - GFF3385 0.82 +0.3 152,027 - -0.6 152,047 + +0.1 152,052 - +0.1 152,246 + -0.0 152,324 - -0.5 152,389 + +1.0 152,412 + +1.2 152,571 + GFF3386 0.35 +1.0 152,718 + GFF3386 0.74 +0.3 152,746 + GFF3386 0.82 +1.8 152,751 + GFF3386 0.83 +0.2 152,812 + +0.7 152,813 - +0.8 152,816 + -1.7 152,816 + +3.6 152,817 - +0.2 153,048 + GFF3387 0.62 +0.3
Or see this region's nucleotide sequence