Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3343 and GFF3344 are separated by 124 nucleotides GFF3344 and GFF3345 are separated by 63 nucleotides GFF3345 and GFF3346 are separated by 236 nucleotides GFF3346 and GFF3347 are separated by 35 nucleotides
GFF3343 - Putative lipoprotein yceB precursor, at 111,717 to 112,277
GFF3343
GFF3344 - Glutaredoxin 2, at 112,402 to 113,049
GFF3344
GFF3345 - MFS superfamily export protein YceL, at 113,113 to 114,321
GFF3345
GFF3346 - Ribosomal-protein-S5p-alanine acetyltransferase, at 114,558 to 115,142
GFF3346
GFF3347 - Protein of unknown function YceH, at 115,178 to 115,825
GFF3347
Position (kb)
113
114
115 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 112.204 kb on + strand, within GFF3343 at 112.204 kb on + strand, within GFF3343 at 112.231 kb on - strand at 112.231 kb on - strand at 112.288 kb on - strand at 112.306 kb on + strand at 112.461 kb on - strand at 112.684 kb on - strand, within GFF3344 at 112.750 kb on + strand, within GFF3344 at 112.763 kb on + strand, within GFF3344 at 112.848 kb on - strand, within GFF3344 at 112.848 kb on - strand, within GFF3344 at 112.957 kb on + strand, within GFF3344 at 112.965 kb on + strand, within GFF3344 at 112.966 kb on - strand, within GFF3344 at 113.034 kb on - strand at 113.037 kb on + strand at 113.039 kb on + strand at 113.039 kb on + strand at 113.086 kb on - strand at 113.096 kb on + strand at 113.096 kb on + strand at 113.106 kb on + strand at 113.158 kb on - strand at 113.321 kb on + strand, within GFF3345 at 113.321 kb on + strand, within GFF3345 at 113.321 kb on + strand, within GFF3345 at 113.321 kb on + strand, within GFF3345 at 113.321 kb on + strand, within GFF3345 at 113.321 kb on + strand, within GFF3345 at 113.322 kb on - strand, within GFF3345 at 113.413 kb on - strand, within GFF3345 at 113.413 kb on - strand, within GFF3345 at 113.576 kb on + strand, within GFF3345 at 113.576 kb on + strand, within GFF3345 at 113.577 kb on - strand, within GFF3345 at 113.577 kb on - strand, within GFF3345 at 113.577 kb on - strand, within GFF3345 at 113.617 kb on + strand, within GFF3345 at 113.635 kb on + strand, within GFF3345 at 113.658 kb on + strand, within GFF3345 at 113.658 kb on + strand, within GFF3345 at 113.658 kb on + strand, within GFF3345 at 113.660 kb on + strand, within GFF3345 at 113.660 kb on + strand, within GFF3345 at 113.661 kb on - strand, within GFF3345 at 113.753 kb on - strand, within GFF3345 at 113.753 kb on - strand, within GFF3345 at 113.809 kb on - strand, within GFF3345 at 113.845 kb on + strand, within GFF3345 at 114.505 kb on + strand at 114.506 kb on - strand at 114.506 kb on - strand at 114.507 kb on + strand at 114.508 kb on - strand at 114.508 kb on - strand at 114.508 kb on - strand at 114.508 kb on - strand at 114.510 kb on + strand at 114.510 kb on + strand at 114.511 kb on - strand at 114.516 kb on + strand at 114.536 kb on + strand at 114.548 kb on + strand at 114.548 kb on + strand at 114.552 kb on + strand at 114.554 kb on + strand at 114.554 kb on + strand at 114.576 kb on - strand at 114.576 kb on - strand at 114.623 kb on - strand, within GFF3346 at 114.948 kb on + strand, within GFF3346 at 114.949 kb on - strand, within GFF3346 at 114.969 kb on - strand, within GFF3346 at 114.990 kb on + strand, within GFF3346 at 114.990 kb on + strand, within GFF3346 at 115.291 kb on + strand, within GFF3347 at 115.292 kb on - strand, within GFF3347
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 112,204 + GFF3343 0.87 -1.2 112,204 + GFF3343 0.87 -0.5 112,231 - +0.1 112,231 - +1.0 112,288 - +0.0 112,306 + -0.2 112,461 - -0.1 112,684 - GFF3344 0.44 +0.1 112,750 + GFF3344 0.54 -0.1 112,763 + GFF3344 0.56 -1.1 112,848 - GFF3344 0.69 +0.1 112,848 - GFF3344 0.69 -0.7 112,957 + GFF3344 0.86 -1.3 112,965 + GFF3344 0.87 +0.0 112,966 - GFF3344 0.87 -0.5 113,034 - +0.7 113,037 + +0.4 113,039 + +0.4 113,039 + +0.1 113,086 - -0.6 113,096 + -0.3 113,096 + -0.2 113,106 + +0.6 113,158 - +0.8 113,321 + GFF3345 0.17 -0.9 113,321 + GFF3345 0.17 +0.8 113,321 + GFF3345 0.17 +0.8 113,321 + GFF3345 0.17 -0.3 113,321 + GFF3345 0.17 +0.3 113,321 + GFF3345 0.17 -0.8 113,322 - GFF3345 0.17 +0.2 113,413 - GFF3345 0.25 +0.5 113,413 - GFF3345 0.25 -0.8 113,576 + GFF3345 0.38 -1.7 113,576 + GFF3345 0.38 -0.1 113,577 - GFF3345 0.38 -1.0 113,577 - GFF3345 0.38 -0.2 113,577 - GFF3345 0.38 -0.6 113,617 + GFF3345 0.42 -0.1 113,635 + GFF3345 0.43 +0.9 113,658 + GFF3345 0.45 -0.5 113,658 + GFF3345 0.45 +0.3 113,658 + GFF3345 0.45 -0.8 113,660 + GFF3345 0.45 +1.7 113,660 + GFF3345 0.45 +0.2 113,661 - GFF3345 0.45 +0.4 113,753 - GFF3345 0.53 +1.2 113,753 - GFF3345 0.53 -2.4 113,809 - GFF3345 0.58 +0.1 113,845 + GFF3345 0.61 -0.3 114,505 + +2.8 114,506 - +0.0 114,506 - -0.0 114,507 + +1.0 114,508 - +0.5 114,508 - +0.5 114,508 - +1.1 114,508 - -1.3 114,510 + -0.7 114,510 + +2.9 114,511 - -0.0 114,516 + +2.0 114,536 + +0.7 114,548 + +0.5 114,548 + +0.1 114,552 + -2.0 114,554 + -0.1 114,554 + -0.0 114,576 - +0.8 114,576 - +0.4 114,623 - GFF3346 0.11 -0.1 114,948 + GFF3346 0.67 -0.3 114,949 - GFF3346 0.67 +0.1 114,969 - GFF3346 0.70 +1.9 114,990 + GFF3346 0.74 +1.0 114,990 + GFF3346 0.74 +1.0 115,291 + GFF3347 0.17 +0.9 115,292 - GFF3347 0.18 -0.3
Or see this region's nucleotide sequence