Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3271 and GFF3272 are separated by 79 nucleotides GFF3272 and GFF3273 are separated by 7 nucleotides GFF3273 and GFF3274 are separated by 135 nucleotides
GFF3271 - Probable dipeptidase (EC 3.4.-.-), at 42,504 to 44,066
GFF3271
GFF3272 - Putative two component system histidine kinase YedV, at 44,146 to 45,495
GFF3272
GFF3273 - Putative two-component system response regulator YedW, at 45,503 to 46,183
GFF3273
GFF3274 - 5-Hydroxyisourate Hydrolase (HIUase) (EC 3.5.2.17), at 46,319 to 46,729
GFF3274
Position (kb) 44
45
46 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 43.381 kb on + strand, within GFF3271 at 43.381 kb on + strand, within GFF3271 at 43.381 kb on + strand, within GFF3271 at 43.439 kb on - strand, within GFF3271 at 43.441 kb on - strand, within GFF3271 at 43.632 kb on - strand, within GFF3271 at 43.634 kb on - strand, within GFF3271 at 43.634 kb on - strand, within GFF3271 at 43.707 kb on - strand, within GFF3271 at 43.730 kb on - strand, within GFF3271 at 43.782 kb on - strand, within GFF3271 at 43.823 kb on + strand, within GFF3271 at 43.998 kb on + strand at 44.147 kb on + strand at 44.147 kb on + strand at 44.187 kb on + strand at 44.289 kb on + strand, within GFF3272 at 44.290 kb on - strand, within GFF3272 at 44.290 kb on - strand, within GFF3272 at 44.399 kb on - strand, within GFF3272 at 44.421 kb on - strand, within GFF3272 at 44.601 kb on + strand, within GFF3272 at 44.946 kb on + strand, within GFF3272 at 45.081 kb on + strand, within GFF3272 at 45.082 kb on - strand, within GFF3272 at 45.084 kb on + strand, within GFF3272 at 45.085 kb on - strand, within GFF3272 at 45.148 kb on + strand, within GFF3272 at 45.164 kb on - strand, within GFF3272 at 45.271 kb on + strand, within GFF3272 at 45.271 kb on + strand, within GFF3272 at 45.384 kb on + strand at 45.395 kb on + strand at 45.422 kb on + strand at 45.423 kb on - strand at 45.505 kb on - strand at 45.646 kb on + strand, within GFF3273 at 45.647 kb on - strand, within GFF3273 at 45.818 kb on - strand, within GFF3273 at 45.886 kb on + strand, within GFF3273 at 45.895 kb on - strand, within GFF3273 at 45.961 kb on + strand, within GFF3273 at 45.961 kb on + strand, within GFF3273 at 45.961 kb on + strand, within GFF3273 at 46.099 kb on - strand, within GFF3273 at 46.111 kb on - strand, within GFF3273 at 46.192 kb on + strand at 46.240 kb on + strand at 46.240 kb on + strand at 46.241 kb on - strand at 46.284 kb on + strand at 46.284 kb on + strand at 46.284 kb on + strand at 46.330 kb on + strand at 46.330 kb on + strand at 46.331 kb on - strand at 46.331 kb on - strand at 46.332 kb on + strand at 46.348 kb on - strand at 46.348 kb on - strand at 46.411 kb on + strand, within GFF3274 at 46.412 kb on - strand, within GFF3274
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 43,381 + GFF3271 0.56 +0.8 43,381 + GFF3271 0.56 -2.1 43,381 + GFF3271 0.56 +1.3 43,439 - GFF3271 0.60 +0.8 43,441 - GFF3271 0.60 +3.4 43,632 - GFF3271 0.72 -0.1 43,634 - GFF3271 0.72 +0.1 43,634 - GFF3271 0.72 -1.7 43,707 - GFF3271 0.77 +0.6 43,730 - GFF3271 0.78 +0.0 43,782 - GFF3271 0.82 -0.2 43,823 + GFF3271 0.84 +0.4 43,998 + +0.0 44,147 + -0.0 44,147 + +0.1 44,187 + -0.2 44,289 + GFF3272 0.11 -0.4 44,290 - GFF3272 0.11 +0.7 44,290 - GFF3272 0.11 +0.9 44,399 - GFF3272 0.19 +0.2 44,421 - GFF3272 0.20 -0.6 44,601 + GFF3272 0.34 +1.3 44,946 + GFF3272 0.59 -1.1 45,081 + GFF3272 0.69 +2.4 45,082 - GFF3272 0.69 +0.7 45,084 + GFF3272 0.69 -0.2 45,085 - GFF3272 0.70 +1.5 45,148 + GFF3272 0.74 +0.6 45,164 - GFF3272 0.75 +1.7 45,271 + GFF3272 0.83 +1.9 45,271 + GFF3272 0.83 -0.1 45,384 + +0.4 45,395 + -0.3 45,422 + -0.0 45,423 - -0.9 45,505 - +1.0 45,646 + GFF3273 0.21 +5.6 45,647 - GFF3273 0.21 -0.7 45,818 - GFF3273 0.46 +0.9 45,886 + GFF3273 0.56 +0.2 45,895 - GFF3273 0.58 +0.1 45,961 + GFF3273 0.67 -0.2 45,961 + GFF3273 0.67 +0.1 45,961 + GFF3273 0.67 +0.5 46,099 - GFF3273 0.88 +0.5 46,111 - GFF3273 0.89 +0.7 46,192 + -0.1 46,240 + -0.1 46,240 + -0.1 46,241 - +0.8 46,284 + +0.4 46,284 + +0.4 46,284 + +0.4 46,330 + +0.1 46,330 + +0.2 46,331 - -0.5 46,331 - +0.7 46,332 + +0.5 46,348 - -1.1 46,348 - -0.3 46,411 + GFF3274 0.22 +0.2 46,412 - GFF3274 0.23 -1.6
Or see this region's nucleotide sequence