Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3251 and GFF3252 are separated by 18 nucleotides GFF3252 and GFF3253 are separated by 123 nucleotides GFF3253 and GFF3254 are separated by 35 nucleotides GFF3254 and GFF3255 are separated by 94 nucleotides
GFF3251 - Putative efflux (PET) family inner membrane protein YccS, at 25,558 to 27,711
GFF3251
GFF3252 - Inner membrane protein YccF, at 27,730 to 28,176
GFF3252
GFF3253 - DNA helicase IV, at 28,300 to 30,354
GFF3253
GFF3254 - Methylglyoxal synthase (EC 4.2.3.3), at 30,390 to 30,848
GFF3254
GFF3255 - UPF0319 protein YccT precursor, at 30,943 to 31,605
GFF3255
Position (kb) 28
29
30
31 Strain fitness (log2 ratio) -1
0
1
2
3 at 27.317 kb on - strand, within GFF3251 at 27.338 kb on + strand, within GFF3251 at 27.428 kb on - strand, within GFF3251 at 27.646 kb on - strand at 27.659 kb on + strand at 27.659 kb on + strand at 27.660 kb on - strand at 27.673 kb on - strand at 27.673 kb on - strand at 27.684 kb on + strand at 27.711 kb on + strand at 28.022 kb on + strand, within GFF3252 at 28.083 kb on + strand, within GFF3252 at 28.093 kb on - strand, within GFF3252 at 28.203 kb on - strand at 28.268 kb on - strand at 28.546 kb on + strand, within GFF3253 at 28.626 kb on + strand, within GFF3253 at 28.626 kb on + strand, within GFF3253 at 28.770 kb on + strand, within GFF3253 at 28.770 kb on + strand, within GFF3253 at 28.828 kb on + strand, within GFF3253 at 28.828 kb on + strand, within GFF3253 at 29.140 kb on + strand, within GFF3253 at 29.143 kb on - strand, within GFF3253 at 29.145 kb on + strand, within GFF3253 at 29.680 kb on - strand, within GFF3253 at 29.884 kb on - strand, within GFF3253 at 29.985 kb on + strand, within GFF3253 at 30.030 kb on - strand, within GFF3253 at 30.236 kb on + strand at 30.237 kb on - strand at 30.239 kb on - strand at 30.392 kb on - strand at 30.442 kb on - strand, within GFF3254 at 30.473 kb on + strand, within GFF3254 at 30.677 kb on + strand, within GFF3254 at 30.721 kb on + strand, within GFF3254 at 30.866 kb on - strand at 31.155 kb on - strand, within GFF3255 at 31.200 kb on + strand, within GFF3255
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 27,317 - GFF3251 0.82 -0.9 27,338 + GFF3251 0.83 -1.0 27,428 - GFF3251 0.87 +0.5 27,646 - +2.9 27,659 + -0.4 27,659 + +0.2 27,660 - -0.1 27,673 - -0.2 27,673 - +0.4 27,684 + -1.0 27,711 + -0.2 28,022 + GFF3252 0.65 -0.5 28,083 + GFF3252 0.79 +1.3 28,093 - GFF3252 0.81 -0.2 28,203 - -0.2 28,268 - +0.5 28,546 + GFF3253 0.12 +0.5 28,626 + GFF3253 0.16 -0.3 28,626 + GFF3253 0.16 +0.2 28,770 + GFF3253 0.23 -0.1 28,770 + GFF3253 0.23 -0.3 28,828 + GFF3253 0.26 -0.2 28,828 + GFF3253 0.26 -0.3 29,140 + GFF3253 0.41 +0.2 29,143 - GFF3253 0.41 +0.7 29,145 + GFF3253 0.41 +0.5 29,680 - GFF3253 0.67 -0.5 29,884 - GFF3253 0.77 -0.4 29,985 + GFF3253 0.82 -0.4 30,030 - GFF3253 0.84 -0.1 30,236 + +0.4 30,237 - +1.1 30,239 - -0.6 30,392 - +0.4 30,442 - GFF3254 0.11 +0.2 30,473 + GFF3254 0.18 -0.5 30,677 + GFF3254 0.63 +0.6 30,721 + GFF3254 0.72 +1.7 30,866 - +1.3 31,155 - GFF3255 0.32 +0.8 31,200 + GFF3255 0.39 +0.5
Or see this region's nucleotide sequence