Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3060 and GFF3061 are separated by 18 nucleotides GFF3061 and GFF3062 are separated by 17 nucleotides GFF3062 and GFF3063 are separated by 49 nucleotides GFF3063 and GFF3064 overlap by 1 nucleotides GFF3064 and GFF3065 are separated by 106 nucleotides
GFF3060 - L-asparaginase (EC 3.5.1.1), at 70,616 to 71,662
GFF3060
GFF3061 - hypothetical protein, at 71,681 to 71,833
GFF3061
GFF3062 - FIG004016: Uncharacterized protein YggN, at 71,851 to 72,570
GFF3062
GFF3063 - FIG002060: uncharacterized protein YggL, at 72,620 to 72,946
GFF3063
GFF3064 - tRNA (guanine46-N7-)-methyltransferase (EC 2.1.1.33), at 72,946 to 73,713
GFF3064
GFF3065 - A/G-specific adenine glycosylase (EC 3.2.2.-), at 73,820 to 74,872
GFF3065
Position (kb) 72
73 Strain fitness (log2 ratio) -1
0
1
2
3 at 71.637 kb on - strand at 71.665 kb on + strand at 71.665 kb on + strand at 71.667 kb on + strand at 71.891 kb on - strand at 71.986 kb on + strand, within GFF3062 at 72.132 kb on + strand, within GFF3062 at 72.135 kb on - strand, within GFF3062 at 72.249 kb on + strand, within GFF3062 at 72.506 kb on + strand at 72.539 kb on - strand at 72.571 kb on - strand at 72.571 kb on - strand at 72.618 kb on + strand at 72.618 kb on + strand at 72.619 kb on - strand at 72.619 kb on - strand at 72.622 kb on - strand at 72.763 kb on - strand, within GFF3063 at 72.808 kb on + strand, within GFF3063 at 72.851 kb on - strand, within GFF3063 at 72.948 kb on - strand at 72.967 kb on + strand at 73.046 kb on + strand, within GFF3064 at 73.047 kb on - strand, within GFF3064 at 73.047 kb on - strand, within GFF3064 at 73.047 kb on - strand, within GFF3064 at 73.084 kb on - strand, within GFF3064 at 73.213 kb on - strand, within GFF3064 at 73.224 kb on + strand, within GFF3064 at 73.284 kb on + strand, within GFF3064 at 73.285 kb on - strand, within GFF3064 at 73.602 kb on + strand, within GFF3064 at 73.717 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 71,637 - +0.3 71,665 + +0.1 71,665 + -0.2 71,667 + -0.2 71,891 - -0.3 71,986 + GFF3062 0.19 -0.9 72,132 + GFF3062 0.39 -0.3 72,135 - GFF3062 0.39 +0.2 72,249 + GFF3062 0.55 -0.0 72,506 + -0.5 72,539 - -0.4 72,571 - +0.4 72,571 - +0.3 72,618 + +0.4 72,618 + -0.6 72,619 - -0.4 72,619 - +0.0 72,622 - +0.5 72,763 - GFF3063 0.44 +1.1 72,808 + GFF3063 0.57 +0.9 72,851 - GFF3063 0.71 +1.3 72,948 - -0.2 72,967 + +0.4 73,046 + GFF3064 0.13 -0.2 73,047 - GFF3064 0.13 +0.1 73,047 - GFF3064 0.13 -0.3 73,047 - GFF3064 0.13 +1.5 73,084 - GFF3064 0.18 +0.8 73,213 - GFF3064 0.35 +3.5 73,224 + GFF3064 0.36 -0.4 73,284 + GFF3064 0.44 +1.6 73,285 - GFF3064 0.44 -0.7 73,602 + GFF3064 0.85 -0.2 73,717 + +0.5
Or see this region's nucleotide sequence