Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF305 and GFF306 are separated by 9 nucleotides GFF306 and GFF307 are separated by 142 nucleotides
GFF305 - Sensor histidine protein kinase UhpB, glucose-6-phosphate specific (EC 2.7.13.3), at 22,524 to 24,026
GFF305
GFF306 - Hexose phosphate uptake regulatory protein UhpC, at 24,036 to 25,364
GFF306
GFF307 - Hexose phosphate transport protein UhpT, at 25,507 to 26,898
GFF307
Position (kb) 24
25
26 Strain fitness (log2 ratio) -2
-1
0
1 at 23.153 kb on + strand, within GFF305 at 23.586 kb on + strand, within GFF305 at 23.587 kb on - strand, within GFF305 at 23.587 kb on - strand, within GFF305 at 23.632 kb on + strand, within GFF305 at 23.641 kb on + strand, within GFF305 at 23.642 kb on - strand, within GFF305 at 23.800 kb on + strand, within GFF305 at 24.019 kb on - strand at 24.173 kb on + strand, within GFF306 at 24.174 kb on - strand, within GFF306 at 24.268 kb on - strand, within GFF306 at 24.406 kb on + strand, within GFF306 at 24.407 kb on - strand, within GFF306 at 24.407 kb on - strand, within GFF306 at 24.555 kb on - strand, within GFF306 at 24.786 kb on + strand, within GFF306 at 24.862 kb on - strand, within GFF306 at 24.863 kb on + strand, within GFF306 at 24.863 kb on + strand, within GFF306 at 24.910 kb on + strand, within GFF306 at 24.910 kb on + strand, within GFF306 at 25.066 kb on - strand, within GFF306 at 25.066 kb on - strand, within GFF306 at 25.066 kb on - strand, within GFF306 at 25.158 kb on - strand, within GFF306 at 25.365 kb on + strand at 25.365 kb on + strand at 25.366 kb on - strand at 25.520 kb on + strand at 25.570 kb on - strand at 25.601 kb on - strand at 25.634 kb on - strand at 25.634 kb on - strand at 25.680 kb on + strand, within GFF307 at 25.739 kb on - strand, within GFF307 at 25.765 kb on + strand, within GFF307 at 25.806 kb on + strand, within GFF307 at 25.830 kb on + strand, within GFF307 at 25.830 kb on + strand, within GFF307 at 25.831 kb on - strand, within GFF307 at 25.894 kb on + strand, within GFF307 at 25.895 kb on - strand, within GFF307 at 26.001 kb on + strand, within GFF307 at 26.059 kb on + strand, within GFF307 at 26.060 kb on - strand, within GFF307 at 26.164 kb on + strand, within GFF307 at 26.328 kb on - strand, within GFF307
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 23,153 + GFF305 0.42 +1.4 23,586 + GFF305 0.71 +0.5 23,587 - GFF305 0.71 +0.9 23,587 - GFF305 0.71 -0.4 23,632 + GFF305 0.74 +0.5 23,641 + GFF305 0.74 -0.2 23,642 - GFF305 0.74 +0.7 23,800 + GFF305 0.85 -0.8 24,019 - +0.2 24,173 + GFF306 0.10 +1.2 24,174 - GFF306 0.10 -0.3 24,268 - GFF306 0.17 -0.7 24,406 + GFF306 0.28 +0.7 24,407 - GFF306 0.28 +0.1 24,407 - GFF306 0.28 +0.0 24,555 - GFF306 0.39 -0.3 24,786 + GFF306 0.56 -0.0 24,862 - GFF306 0.62 -0.5 24,863 + GFF306 0.62 +0.1 24,863 + GFF306 0.62 -0.7 24,910 + GFF306 0.66 -0.6 24,910 + GFF306 0.66 -0.2 25,066 - GFF306 0.78 +0.3 25,066 - GFF306 0.78 -0.6 25,066 - GFF306 0.78 -0.4 25,158 - GFF306 0.84 -0.4 25,365 + -0.3 25,365 + -0.1 25,366 - +1.5 25,520 + -0.3 25,570 - +1.7 25,601 - -0.3 25,634 - +0.2 25,634 - +0.3 25,680 + GFF307 0.12 +0.5 25,739 - GFF307 0.17 -1.0 25,765 + GFF307 0.19 -0.6 25,806 + GFF307 0.21 +0.3 25,830 + GFF307 0.23 -0.0 25,830 + GFF307 0.23 -0.6 25,831 - GFF307 0.23 -2.4 25,894 + GFF307 0.28 -0.4 25,895 - GFF307 0.28 -1.1 26,001 + GFF307 0.35 -0.9 26,059 + GFF307 0.40 -0.5 26,060 - GFF307 0.40 +0.7 26,164 + GFF307 0.47 -0.6 26,328 - GFF307 0.59 +1.6
Or see this region's nucleotide sequence