Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3054 and GFF3055 are separated by 18 nucleotides GFF3055 and GFF3056 overlap by 4 nucleotides GFF3056 and GFF3057 are separated by 7 nucleotides GFF3057 and GFF3058 overlap by 8 nucleotides
GFF3054 - Hypothetical protein YggS, proline synthase co-transcribed bacterial homolog PROSC, at 66,079 to 66,783
GFF3054
GFF3055 - Integral membrane protein YggT, involved in response to extracytoplasmic stress (osmotic shock), at 66,802 to 67,368
GFF3055
GFF3056 - COG1872, at 67,365 to 67,655
GFF3056
GFF3057 - Nucleoside 5-triphosphatase RdgB (dHAPTP, dITP, XTP-specific) (EC 3.6.1.15), at 67,663 to 68,256
GFF3057
GFF3058 - Radical SAM family enzyme, similar to coproporphyrinogen III oxidase, oxygen-independent, clustered with nucleoside-triphosphatase RdgB, at 68,249 to 69,385
GFF3058
Position (kb) 67
68
69 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 66.677 kb on + strand, within GFF3054 at 66.678 kb on - strand, within GFF3054 at 66.687 kb on + strand, within GFF3054 at 66.723 kb on + strand at 66.844 kb on + strand at 66.844 kb on + strand at 66.844 kb on + strand at 66.846 kb on + strand at 66.857 kb on + strand at 67.017 kb on + strand, within GFF3055 at 67.089 kb on + strand, within GFF3055 at 67.089 kb on + strand, within GFF3055 at 67.098 kb on + strand, within GFF3055 at 67.183 kb on + strand, within GFF3055 at 67.183 kb on + strand, within GFF3055 at 67.184 kb on - strand, within GFF3055 at 67.187 kb on + strand, within GFF3055 at 67.187 kb on + strand, within GFF3055 at 67.188 kb on - strand, within GFF3055 at 67.288 kb on + strand, within GFF3055 at 67.289 kb on - strand, within GFF3055 at 67.289 kb on - strand, within GFF3055 at 67.300 kb on - strand, within GFF3055 at 67.319 kb on + strand at 67.319 kb on + strand at 67.439 kb on + strand, within GFF3056 at 67.653 kb on + strand at 67.657 kb on + strand at 67.912 kb on + strand, within GFF3057 at 67.913 kb on - strand, within GFF3057 at 67.924 kb on + strand, within GFF3057 at 67.980 kb on - strand, within GFF3057 at 68.148 kb on - strand, within GFF3057 at 68.255 kb on - strand at 68.278 kb on + strand at 68.278 kb on + strand at 68.285 kb on - strand at 68.806 kb on + strand, within GFF3058 at 68.929 kb on - strand, within GFF3058 at 68.931 kb on + strand, within GFF3058 at 69.008 kb on + strand, within GFF3058 at 69.118 kb on + strand, within GFF3058 at 69.119 kb on - strand, within GFF3058
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 66,677 + GFF3054 0.85 +0.2 66,678 - GFF3054 0.85 -0.7 66,687 + GFF3054 0.86 -0.4 66,723 + -0.6 66,844 + +0.2 66,844 + -0.4 66,844 + +0.1 66,846 + +0.1 66,857 + +0.0 67,017 + GFF3055 0.38 -0.7 67,089 + GFF3055 0.51 -0.2 67,089 + GFF3055 0.51 +0.3 67,098 + GFF3055 0.52 -1.1 67,183 + GFF3055 0.67 +0.7 67,183 + GFF3055 0.67 +2.5 67,184 - GFF3055 0.67 -0.7 67,187 + GFF3055 0.68 -0.0 67,187 + GFF3055 0.68 -1.0 67,188 - GFF3055 0.68 -0.2 67,288 + GFF3055 0.86 -0.4 67,289 - GFF3055 0.86 -0.3 67,289 - GFF3055 0.86 -1.1 67,300 - GFF3055 0.88 -0.1 67,319 + +1.3 67,319 + +0.0 67,439 + GFF3056 0.25 -0.2 67,653 + -1.7 67,657 + +0.8 67,912 + GFF3057 0.42 -1.0 67,913 - GFF3057 0.42 -0.5 67,924 + GFF3057 0.44 -1.0 67,980 - GFF3057 0.53 -0.5 68,148 - GFF3057 0.82 -0.7 68,255 - -0.1 68,278 + -0.5 68,278 + -1.7 68,285 - -0.3 68,806 + GFF3058 0.49 -1.0 68,929 - GFF3058 0.60 +0.2 68,931 + GFF3058 0.60 +1.9 69,008 + GFF3058 0.67 -0.3 69,118 + GFF3058 0.76 -2.8 69,119 - GFF3058 0.77 +0.8
Or see this region's nucleotide sequence