Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF284 and GFF285 are separated by 109 nucleotides GFF285 and GFF286 are separated by 189 nucleotides GFF286 and GFF287 are separated by 161 nucleotides GFF287 and GFF288 overlap by 19 nucleotides GFF288 and GFF289 are separated by 19 nucleotides GFF289 and GFF290 overlap by 11 nucleotides
GFF284 - 16 kDa heat shock protein A, at 2,743 to 3,162
GFF284
GFF285 - 16 kDa heat shock protein B, at 3,272 to 3,700
GFF285
GFF286 - Mediator of hyperadherence YidE, at 3,890 to 5,551
GFF286
GFF287 - hypothetical protein, at 5,713 to 5,859
GFF287
GFF288 - hypothetical protein, at 5,841 to 5,975
GFF288
GFF289 - Putative inner membrane protein, at 5,995 to 6,342
GFF289
GFF290 - Putative inner membrane protein, at 6,332 to 6,694
GFF290
Position (kb)
3
4
5
6 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2.997 kb on + strand, within GFF284 at 2.998 kb on - strand, within GFF284 at 2.998 kb on - strand, within GFF284 at 3.001 kb on + strand, within GFF284 at 3.001 kb on + strand, within GFF284 at 3.002 kb on - strand, within GFF284 at 3.039 kb on + strand, within GFF284 at 3.039 kb on + strand, within GFF284 at 3.100 kb on + strand, within GFF284 at 3.100 kb on + strand, within GFF284 at 3.101 kb on - strand, within GFF284 at 3.102 kb on + strand, within GFF284 at 3.255 kb on + strand at 3.256 kb on - strand at 3.271 kb on + strand at 3.278 kb on - strand at 3.281 kb on + strand at 3.282 kb on - strand at 3.376 kb on + strand, within GFF285 at 3.413 kb on - strand, within GFF285 at 3.620 kb on - strand, within GFF285 at 3.690 kb on + strand at 3.695 kb on - strand at 3.698 kb on + strand at 3.699 kb on - strand at 3.699 kb on - strand at 3.824 kb on + strand at 3.824 kb on + strand at 3.825 kb on - strand at 3.825 kb on - strand at 3.826 kb on + strand at 3.826 kb on + strand at 3.827 kb on - strand at 3.827 kb on - strand at 3.827 kb on - strand at 3.828 kb on + strand at 3.828 kb on + strand at 3.828 kb on + strand at 3.829 kb on - strand at 3.961 kb on + strand at 4.012 kb on + strand at 4.195 kb on + strand, within GFF286 at 4.368 kb on - strand, within GFF286 at 4.368 kb on - strand, within GFF286 at 4.380 kb on - strand, within GFF286 at 4.497 kb on + strand, within GFF286 at 4.540 kb on + strand, within GFF286 at 4.540 kb on + strand, within GFF286 at 4.543 kb on + strand, within GFF286 at 4.543 kb on + strand, within GFF286 at 4.543 kb on + strand, within GFF286 at 4.543 kb on + strand, within GFF286 at 4.756 kb on - strand, within GFF286 at 4.823 kb on - strand, within GFF286 at 5.028 kb on + strand, within GFF286 at 5.156 kb on + strand, within GFF286 at 5.200 kb on - strand, within GFF286 at 5.295 kb on - strand, within GFF286 at 5.299 kb on + strand, within GFF286 at 5.299 kb on + strand, within GFF286 at 5.503 kb on + strand at 5.503 kb on + strand at 5.504 kb on - strand at 5.592 kb on + strand at 5.714 kb on + strand at 5.818 kb on - strand, within GFF287 at 5.878 kb on + strand, within GFF288 at 5.885 kb on - strand, within GFF288 at 5.895 kb on - strand, within GFF288 at 5.970 kb on - strand at 5.973 kb on + strand at 5.973 kb on + strand at 5.973 kb on + strand at 5.974 kb on - strand at 5.974 kb on - strand at 6.199 kb on + strand, within GFF289 at 6.200 kb on - strand, within GFF289 at 6.200 kb on - strand, within GFF289 at 6.209 kb on + strand, within GFF289 at 6.261 kb on + strand, within GFF289 at 6.267 kb on + strand, within GFF289 at 6.311 kb on + strand at 6.347 kb on - strand at 6.388 kb on + strand, within GFF290 at 6.389 kb on - strand, within GFF290 at 6.422 kb on + strand, within GFF290 at 6.532 kb on + strand, within GFF290
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 2,997 + GFF284 0.60 -0.1 2,998 - GFF284 0.61 -0.3 2,998 - GFF284 0.61 -0.7 3,001 + GFF284 0.61 -0.7 3,001 + GFF284 0.61 +0.1 3,002 - GFF284 0.62 +0.6 3,039 + GFF284 0.70 -0.4 3,039 + GFF284 0.70 -0.4 3,100 + GFF284 0.85 +0.1 3,100 + GFF284 0.85 -0.8 3,101 - GFF284 0.85 +0.7 3,102 + GFF284 0.85 -0.8 3,255 + -1.9 3,256 - +1.3 3,271 + +1.0 3,278 - -0.7 3,281 + -0.3 3,282 - +0.4 3,376 + GFF285 0.24 -0.7 3,413 - GFF285 0.33 -0.6 3,620 - GFF285 0.81 -0.7 3,690 + +1.8 3,695 - +0.5 3,698 + +0.4 3,699 - +1.4 3,699 - -0.2 3,824 + -0.0 3,824 + +0.5 3,825 - +0.1 3,825 - +0.3 3,826 + +2.4 3,826 + +1.0 3,827 - -0.5 3,827 - +0.6 3,827 - +0.0 3,828 + -0.1 3,828 + +1.2 3,828 + -0.4 3,829 - -0.4 3,961 + -0.7 4,012 + -0.1 4,195 + GFF286 0.18 +0.0 4,368 - GFF286 0.29 +0.7 4,368 - GFF286 0.29 +0.7 4,380 - GFF286 0.29 -0.5 4,497 + GFF286 0.37 -0.7 4,540 + GFF286 0.39 +0.5 4,540 + GFF286 0.39 -0.6 4,543 + GFF286 0.39 -0.2 4,543 + GFF286 0.39 -0.9 4,543 + GFF286 0.39 -0.7 4,543 + GFF286 0.39 -0.4 4,756 - GFF286 0.52 +0.5 4,823 - GFF286 0.56 -0.6 5,028 + GFF286 0.68 -0.5 5,156 + GFF286 0.76 -0.2 5,200 - GFF286 0.79 -1.2 5,295 - GFF286 0.85 +1.5 5,299 + GFF286 0.85 -0.8 5,299 + GFF286 0.85 -0.3 5,503 + +1.5 5,503 + -1.7 5,504 - +0.0 5,592 + +0.0 5,714 + +1.5 5,818 - GFF287 0.71 -1.6 5,878 + GFF288 0.27 +2.2 5,885 - GFF288 0.33 -0.5 5,895 - GFF288 0.40 -0.5 5,970 - +0.1 5,973 + -0.7 5,973 + -0.5 5,973 + +0.7 5,974 - +0.5 5,974 - -0.3 6,199 + GFF289 0.59 +2.7 6,200 - GFF289 0.59 -0.0 6,200 - GFF289 0.59 +1.2 6,209 + GFF289 0.61 +0.3 6,261 + GFF289 0.76 +0.1 6,267 + GFF289 0.78 -0.8 6,311 + +1.0 6,347 - +0.8 6,388 + GFF290 0.15 -1.2 6,389 - GFF290 0.16 -0.1 6,422 + GFF290 0.25 +0.1 6,532 + GFF290 0.55 -0.4
Or see this region's nucleotide sequence