Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2791 and GFF2792 overlap by 4 nucleotides GFF2792 and GFF2793 are separated by 74 nucleotides GFF2793 and GFF2794 are separated by 11 nucleotides
GFF2791 - Hydrogenase maturation factor hoxO, at 314,338 to 314,742
GFF2791
GFF2792 - Hydrogenase maturation factor HoxQ, at 314,739 to 315,587
GFF2792
GFF2793 - Cytochrome d ubiquinol oxidase subunit I (EC 1.10.3.-), at 315,662 to 317,206
GFF2793
GFF2794 - Cytochrome d ubiquinol oxidase subunit II (EC 1.10.3.-), at 317,218 to 318,354
GFF2794
Position (kb) 315
316
317
318 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 314.736 kb on - strand at 314.775 kb on - strand at 314.846 kb on + strand, within GFF2792 at 314.962 kb on + strand, within GFF2792 at 314.963 kb on - strand, within GFF2792 at 314.999 kb on + strand, within GFF2792 at 315.479 kb on + strand, within GFF2792 at 315.483 kb on + strand, within GFF2792 at 315.484 kb on - strand, within GFF2792 at 315.626 kb on + strand at 315.779 kb on + strand at 315.779 kb on + strand at 315.780 kb on - strand at 315.780 kb on - strand at 315.898 kb on + strand, within GFF2793 at 315.911 kb on + strand, within GFF2793 at 315.921 kb on - strand, within GFF2793 at 316.027 kb on + strand, within GFF2793 at 316.235 kb on + strand, within GFF2793 at 316.835 kb on - strand, within GFF2793 at 316.837 kb on + strand, within GFF2793 at 316.838 kb on - strand, within GFF2793 at 316.863 kb on + strand, within GFF2793 at 316.888 kb on - strand, within GFF2793 at 316.988 kb on - strand, within GFF2793 at 317.147 kb on + strand at 317.228 kb on - strand at 317.452 kb on + strand, within GFF2794 at 317.708 kb on + strand, within GFF2794 at 317.727 kb on - strand, within GFF2794 at 317.760 kb on + strand, within GFF2794 at 317.994 kb on - strand, within GFF2794 at 318.171 kb on + strand, within GFF2794
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 314,736 - -0.2 314,775 - +0.1 314,846 + GFF2792 0.13 -0.0 314,962 + GFF2792 0.26 +0.7 314,963 - GFF2792 0.26 -0.2 314,999 + GFF2792 0.31 +2.3 315,479 + GFF2792 0.87 -0.1 315,483 + GFF2792 0.88 -0.1 315,484 - GFF2792 0.88 +0.1 315,626 + +0.7 315,779 + -0.0 315,779 + +1.0 315,780 - +0.1 315,780 - +0.6 315,898 + GFF2793 0.15 -0.0 315,911 + GFF2793 0.16 +0.4 315,921 - GFF2793 0.17 +0.8 316,027 + GFF2793 0.24 +0.3 316,235 + GFF2793 0.37 -0.4 316,835 - GFF2793 0.76 +0.5 316,837 + GFF2793 0.76 +0.3 316,838 - GFF2793 0.76 +1.0 316,863 + GFF2793 0.78 +0.0 316,888 - GFF2793 0.79 -0.0 316,988 - GFF2793 0.86 +0.3 317,147 + +0.2 317,228 - +1.3 317,452 + GFF2794 0.21 -3.7 317,708 + GFF2794 0.43 -0.2 317,727 - GFF2794 0.45 -1.2 317,760 + GFF2794 0.48 +1.2 317,994 - GFF2794 0.68 +0.2 318,171 + GFF2794 0.84 +0.9
Or see this region's nucleotide sequence