Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2731 and GFF2732 are separated by 337 nucleotides GFF2732 and GFF2733 are separated by 25 nucleotides GFF2733 and GFF2734 are separated by 56 nucleotides GFF2734 and GFF2735 are separated by 169 nucleotides
GFF2731 - Outer membrane protein W precursor, at 253,511 to 254,149
GFF2731
GFF2732 - Ferredoxin, at 254,487 to 254,891
GFF2732
GFF2733 - Membrane protein YciC, linked to IspA, at 254,917 to 255,660
GFF2733
GFF2734 - Intracellular septation protein IspA, at 255,717 to 256,256
GFF2734
GFF2735 - Acyl-CoA thioesterase YciA, involved in membrane biogenesis, at 256,426 to 256,818
GFF2735
Position (kb) 254
255
256 Strain fitness (log2 ratio) -1
0
1
2
3 at 253.954 kb on - strand, within GFF2731 at 254.004 kb on - strand, within GFF2731 at 254.035 kb on + strand, within GFF2731 at 254.066 kb on - strand, within GFF2731 at 254.320 kb on + strand at 254.451 kb on + strand at 254.461 kb on + strand at 254.533 kb on - strand, within GFF2732 at 254.533 kb on - strand, within GFF2732 at 254.620 kb on + strand, within GFF2732 at 254.620 kb on + strand, within GFF2732 at 254.628 kb on - strand, within GFF2732 at 254.628 kb on - strand, within GFF2732 at 254.822 kb on + strand, within GFF2732 at 254.835 kb on - strand, within GFF2732 at 254.890 kb on - strand at 255.012 kb on + strand, within GFF2733 at 255.298 kb on - strand, within GFF2733 at 255.372 kb on + strand, within GFF2733 at 255.373 kb on - strand, within GFF2733 at 255.625 kb on + strand at 255.640 kb on + strand at 255.642 kb on + strand at 255.642 kb on + strand at 255.642 kb on + strand at 255.642 kb on + strand at 255.642 kb on + strand at 255.643 kb on - strand at 255.658 kb on + strand at 255.783 kb on + strand, within GFF2734 at 255.876 kb on - strand, within GFF2734 at 255.988 kb on + strand, within GFF2734 at 256.254 kb on + strand at 256.271 kb on - strand at 256.434 kb on + strand at 256.458 kb on - strand at 256.461 kb on + strand at 256.581 kb on - strand, within GFF2735 at 256.638 kb on - strand, within GFF2735
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 253,954 - GFF2731 0.69 +0.2 254,004 - GFF2731 0.77 +1.9 254,035 + GFF2731 0.82 +2.2 254,066 - GFF2731 0.87 -0.8 254,320 + -0.0 254,451 + +2.5 254,461 + +0.3 254,533 - GFF2732 0.11 -0.2 254,533 - GFF2732 0.11 -0.4 254,620 + GFF2732 0.33 -0.4 254,620 + GFF2732 0.33 -1.1 254,628 - GFF2732 0.35 -0.4 254,628 - GFF2732 0.35 -1.2 254,822 + GFF2732 0.83 -0.2 254,835 - GFF2732 0.86 +0.5 254,890 - +0.2 255,012 + GFF2733 0.13 -1.1 255,298 - GFF2733 0.51 -0.0 255,372 + GFF2733 0.61 +0.0 255,373 - GFF2733 0.61 -1.7 255,625 + -0.4 255,640 + +1.2 255,642 + +0.8 255,642 + -0.2 255,642 + -0.0 255,642 + +0.1 255,642 + +2.0 255,643 - -0.6 255,658 + -0.5 255,783 + GFF2734 0.12 -0.9 255,876 - GFF2734 0.29 +1.6 255,988 + GFF2734 0.50 -0.8 256,254 + -0.6 256,271 - +1.4 256,434 + +0.6 256,458 - +2.8 256,461 + +0.1 256,581 - GFF2735 0.39 -0.4 256,638 - GFF2735 0.54 +0.6
Or see this region's nucleotide sequence