Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2707 and GFF2708 are separated by 108 nucleotides GFF2708 and GFF2709 are separated by 55 nucleotides GFF2709 and GFF2710 are separated by 384 nucleotides GFF2710 and GFF2711 are separated by 0 nucleotides GFF2711 and GFF2712 are separated by 292 nucleotides
GFF2707 - Phosphatidylglycerophosphatase B (EC 3.1.3.27), at 228,767 to 229,531
GFF2707
GFF2708 - GTP cyclohydrolase II (EC 3.5.4.25), at 229,640 to 230,317
GFF2708
GFF2709 - Aconitate hydratase (EC 4.2.1.3) @ 2-methylisocitrate dehydratase (EC 4.2.1.99), at 230,373 to 233,048
GFF2709
GFF2710 - FIG01045680: hypothetical protein, at 233,433 to 233,603
GFF2710
GFF2711 - hypothetical protein, at 233,604 to 233,732
GFF2711
GFF2712 - Cys regulon transcriptional activator CysB, at 234,025 to 234,999
GFF2712
Position (kb) 230
231
232
233
234 Strain fitness (log2 ratio) -1
0
1
2
3 at 229.551 kb on - strand at 229.555 kb on - strand at 229.623 kb on + strand at 229.674 kb on + strand at 230.307 kb on + strand at 230.307 kb on + strand at 230.308 kb on - strand at 230.312 kb on - strand at 230.329 kb on + strand at 230.385 kb on - strand at 230.391 kb on - strand at 230.425 kb on + strand at 230.735 kb on - strand, within GFF2709 at 231.003 kb on - strand, within GFF2709 at 231.021 kb on - strand, within GFF2709 at 231.059 kb on + strand, within GFF2709 at 231.134 kb on - strand, within GFF2709 at 231.220 kb on + strand, within GFF2709 at 231.237 kb on - strand, within GFF2709 at 231.353 kb on + strand, within GFF2709 at 231.567 kb on - strand, within GFF2709 at 231.567 kb on - strand, within GFF2709 at 231.702 kb on + strand, within GFF2709 at 231.703 kb on - strand, within GFF2709 at 231.976 kb on - strand, within GFF2709 at 232.022 kb on - strand, within GFF2709 at 232.070 kb on + strand, within GFF2709 at 232.071 kb on - strand, within GFF2709 at 232.073 kb on - strand, within GFF2709 at 232.134 kb on - strand, within GFF2709 at 232.134 kb on - strand, within GFF2709 at 232.134 kb on - strand, within GFF2709 at 232.220 kb on + strand, within GFF2709 at 232.381 kb on - strand, within GFF2709 at 232.406 kb on + strand, within GFF2709 at 232.580 kb on - strand, within GFF2709 at 232.580 kb on - strand, within GFF2709 at 232.580 kb on - strand, within GFF2709 at 232.985 kb on - strand at 232.990 kb on + strand at 232.990 kb on + strand at 233.048 kb on + strand at 233.048 kb on + strand at 233.048 kb on + strand at 233.152 kb on + strand at 233.276 kb on + strand at 233.278 kb on + strand at 233.306 kb on + strand at 233.307 kb on - strand at 233.420 kb on - strand at 233.541 kb on - strand, within GFF2710 at 233.635 kb on - strand, within GFF2711 at 233.733 kb on + strand at 233.955 kb on - strand at 234.003 kb on - strand at 234.044 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 229,551 - -0.1 229,555 - -0.1 229,623 + -0.1 229,674 + -0.6 230,307 + -0.2 230,307 + +0.8 230,308 - +0.2 230,312 - +1.5 230,329 + -0.2 230,385 - -0.4 230,391 - -0.2 230,425 + -0.3 230,735 - GFF2709 0.14 +3.4 231,003 - GFF2709 0.24 +2.6 231,021 - GFF2709 0.24 -0.7 231,059 + GFF2709 0.26 +0.1 231,134 - GFF2709 0.28 -0.4 231,220 + GFF2709 0.32 +1.0 231,237 - GFF2709 0.32 +1.5 231,353 + GFF2709 0.37 -0.0 231,567 - GFF2709 0.45 -0.5 231,567 - GFF2709 0.45 -0.3 231,702 + GFF2709 0.50 +0.1 231,703 - GFF2709 0.50 +0.7 231,976 - GFF2709 0.60 +0.4 232,022 - GFF2709 0.62 +0.5 232,070 + GFF2709 0.63 +0.3 232,071 - GFF2709 0.63 -0.3 232,073 - GFF2709 0.64 -0.6 232,134 - GFF2709 0.66 +2.2 232,134 - GFF2709 0.66 +0.8 232,134 - GFF2709 0.66 +0.6 232,220 + GFF2709 0.69 -0.8 232,381 - GFF2709 0.75 +0.4 232,406 + GFF2709 0.76 -0.5 232,580 - GFF2709 0.82 -0.5 232,580 - GFF2709 0.82 +0.0 232,580 - GFF2709 0.82 -0.5 232,985 - +1.6 232,990 + +0.1 232,990 + -0.2 233,048 + -0.2 233,048 + +1.0 233,048 + -0.6 233,152 + +0.2 233,276 + +0.6 233,278 + +0.7 233,306 + -0.4 233,307 - +0.2 233,420 - +0.5 233,541 - GFF2710 0.63 -0.4 233,635 - GFF2711 0.24 +0.5 233,733 + -0.1 233,955 - +1.8 234,003 - +0.1 234,044 - -0.2
Or see this region's nucleotide sequence