Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2549 and GFF2550 overlap by 4 nucleotides GFF2550 and GFF2551 are separated by 128 nucleotides
GFF2549 - Malto-oligosyltrehalose synthase (EC 5.4.99.15), at 63,809 to 66,337
GFF2549
GFF2550 - Malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141), at 66,334 to 68,118
GFF2550
GFF2551 - Putative outer membrane or secreted lipoprotein, at 68,247 to 69,032
GFF2551
Position (kb)
66
67
68
69 Strain fitness (log2 ratio)
-1
0
1
2 at 65.360 kb on + strand, within GFF2549 at 65.391 kb on - strand, within GFF2549 at 65.553 kb on + strand, within GFF2549 at 65.554 kb on - strand, within GFF2549 at 65.554 kb on - strand, within GFF2549 at 65.645 kb on - strand, within GFF2549 at 65.681 kb on + strand, within GFF2549 at 65.783 kb on - strand, within GFF2549 at 65.854 kb on - strand, within GFF2549 at 65.858 kb on - strand, within GFF2549 at 65.858 kb on - strand, within GFF2549 at 65.865 kb on + strand, within GFF2549 at 66.151 kb on + strand at 66.231 kb on + strand at 66.232 kb on - strand at 66.262 kb on - strand at 66.262 kb on - strand at 66.319 kb on - strand at 66.369 kb on + strand at 66.449 kb on - strand at 66.466 kb on - strand at 67.038 kb on - strand, within GFF2550 at 67.276 kb on - strand, within GFF2550 at 67.311 kb on + strand, within GFF2550 at 67.361 kb on + strand, within GFF2550 at 67.422 kb on - strand, within GFF2550 at 67.827 kb on - strand, within GFF2550 at 67.847 kb on + strand, within GFF2550 at 67.928 kb on + strand, within GFF2550 at 68.079 kb on - strand at 68.182 kb on - strand at 68.248 kb on + strand at 68.645 kb on - strand, within GFF2551 at 68.708 kb on + strand, within GFF2551 at 68.713 kb on + strand, within GFF2551 at 68.713 kb on + strand, within GFF2551 at 68.756 kb on - strand, within GFF2551 at 68.797 kb on + strand, within GFF2551 at 68.798 kb on - strand, within GFF2551 at 68.862 kb on - strand, within GFF2551 at 68.862 kb on - strand, within GFF2551 at 68.923 kb on - strand, within GFF2551 at 68.951 kb on + strand, within GFF2551
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 65,360 + GFF2549 0.61 +1.7 65,391 - GFF2549 0.63 -0.0 65,553 + GFF2549 0.69 -0.4 65,554 - GFF2549 0.69 +0.6 65,554 - GFF2549 0.69 +1.1 65,645 - GFF2549 0.73 +0.4 65,681 + GFF2549 0.74 -0.2 65,783 - GFF2549 0.78 -0.0 65,854 - GFF2549 0.81 -0.8 65,858 - GFF2549 0.81 +0.1 65,858 - GFF2549 0.81 -0.5 65,865 + GFF2549 0.81 -0.0 66,151 + -0.2 66,231 + +1.0 66,232 - +1.2 66,262 - -0.1 66,262 - -0.3 66,319 - -0.7 66,369 + +0.6 66,449 - -0.1 66,466 - -0.1 67,038 - GFF2550 0.39 +0.2 67,276 - GFF2550 0.53 -0.1 67,311 + GFF2550 0.55 -0.5 67,361 + GFF2550 0.58 +0.5 67,422 - GFF2550 0.61 -0.1 67,827 - GFF2550 0.84 +1.0 67,847 + GFF2550 0.85 -0.9 67,928 + GFF2550 0.89 +2.7 68,079 - -0.1 68,182 - +0.4 68,248 + +0.3 68,645 - GFF2551 0.51 +0.8 68,708 + GFF2551 0.59 -0.1 68,713 + GFF2551 0.59 +1.4 68,713 + GFF2551 0.59 +0.8 68,756 - GFF2551 0.65 +0.4 68,797 + GFF2551 0.70 +0.7 68,798 - GFF2551 0.70 -0.6 68,862 - GFF2551 0.78 -0.8 68,862 - GFF2551 0.78 -0.6 68,923 - GFF2551 0.86 -1.3 68,951 + GFF2551 0.90 +0.4
Or see this region's nucleotide sequence