Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2525 and GFF2526 are separated by 238 nucleotides GFF2526 and GFF2527 are separated by 148 nucleotides GFF2527 and GFF2528 are separated by 45 nucleotides GFF2528 and GFF2529 are separated by 58 nucleotides
GFF2525 - Uptake hydrogenase small subunit precursor (EC 1.12.99.6), at 39,418 to 40,410
GFF2525
GFF2526 - hypothetical protein, at 40,649 to 40,801
GFF2526
GFF2527 - Choloylglycine hydrolase (EC 3.5.1.24), at 40,950 to 42,041
GFF2527
GFF2528 - Hexuronate utilization operon transcriptional repressor ExuR, at 42,087 to 42,878
GFF2528
GFF2529 - L-galactonate dehydrogenase (EC 1.1.1.-), at 42,937 to 43,962
GFF2529
Position (kb) 40
41
42
43 Strain fitness (log2 ratio) -1
0
1
2 at 39.999 kb on + strand, within GFF2525 at 40.188 kb on - strand, within GFF2525 at 40.357 kb on + strand at 40.378 kb on - strand at 40.392 kb on - strand at 40.397 kb on - strand at 40.397 kb on - strand at 40.542 kb on - strand at 40.543 kb on + strand at 40.722 kb on - strand, within GFF2526 at 41.322 kb on + strand, within GFF2527 at 41.740 kb on - strand, within GFF2527 at 41.969 kb on + strand at 42.215 kb on - strand, within GFF2528 at 42.282 kb on - strand, within GFF2528 at 42.686 kb on + strand, within GFF2528 at 42.707 kb on + strand, within GFF2528 at 42.717 kb on + strand, within GFF2528 at 42.762 kb on - strand, within GFF2528 at 42.954 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 39,999 + GFF2525 0.59 +0.3 40,188 - GFF2525 0.78 +1.8 40,357 + -0.4 40,378 - -1.0 40,392 - +1.3 40,397 - +0.4 40,397 - +0.3 40,542 - +0.7 40,543 + +1.3 40,722 - GFF2526 0.48 +0.3 41,322 + GFF2527 0.34 -0.0 41,740 - GFF2527 0.72 +0.1 41,969 + +0.0 42,215 - GFF2528 0.16 -0.8 42,282 - GFF2528 0.25 +0.2 42,686 + GFF2528 0.76 -1.2 42,707 + GFF2528 0.78 -1.3 42,717 + GFF2528 0.80 -0.9 42,762 - GFF2528 0.85 -0.5 42,954 + -0.5
Or see this region's nucleotide sequence