Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2351 and GFF2352 are separated by 373 nucleotides GFF2352 and GFF2353 are separated by 13 nucleotides
GFF2351 - tRNA (uracil(54)-C5)-methyltransferase (EC 2.1.1.35), at 145,560 to 146,660
GFF2351
GFF2352 - Outer membrane vitamin B12 receptor BtuB, at 147,034 to 148,878
GFF2352
GFF2353 - Glutamate racemase (EC 5.1.1.3), at 148,892 to 149,674
GFF2353
Position (kb)
147
148
149 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 146.072 kb on + strand, within GFF2351 at 146.120 kb on - strand, within GFF2351 at 146.160 kb on - strand, within GFF2351 at 146.160 kb on - strand, within GFF2351 at 146.285 kb on + strand, within GFF2351 at 146.290 kb on + strand, within GFF2351 at 146.332 kb on + strand, within GFF2351 at 146.352 kb on - strand, within GFF2351 at 146.405 kb on + strand, within GFF2351 at 146.474 kb on + strand, within GFF2351 at 146.474 kb on + strand, within GFF2351 at 146.474 kb on + strand, within GFF2351 at 146.475 kb on - strand, within GFF2351 at 146.482 kb on + strand, within GFF2351 at 146.526 kb on - strand, within GFF2351 at 146.526 kb on - strand, within GFF2351 at 146.554 kb on - strand at 146.588 kb on + strand at 146.589 kb on - strand at 146.629 kb on + strand at 146.629 kb on + strand at 146.630 kb on - strand at 146.630 kb on - strand at 146.630 kb on - strand at 146.630 kb on - strand at 146.630 kb on - strand at 146.723 kb on - strand at 146.805 kb on + strand at 146.805 kb on + strand at 146.806 kb on - strand at 146.806 kb on - strand at 146.871 kb on - strand at 146.871 kb on - strand at 146.985 kb on - strand at 147.015 kb on - strand at 147.015 kb on - strand at 147.016 kb on + strand at 147.016 kb on + strand at 147.320 kb on + strand, within GFF2352 at 147.320 kb on + strand, within GFF2352 at 147.321 kb on - strand, within GFF2352 at 147.321 kb on - strand, within GFF2352 at 147.418 kb on + strand, within GFF2352 at 147.418 kb on + strand, within GFF2352 at 147.418 kb on + strand, within GFF2352 at 147.477 kb on + strand, within GFF2352 at 147.483 kb on + strand, within GFF2352 at 147.534 kb on + strand, within GFF2352 at 147.534 kb on + strand, within GFF2352 at 147.691 kb on - strand, within GFF2352 at 147.701 kb on - strand, within GFF2352 at 147.717 kb on + strand, within GFF2352 at 147.788 kb on - strand, within GFF2352 at 147.874 kb on - strand, within GFF2352 at 147.874 kb on - strand, within GFF2352 at 147.898 kb on + strand, within GFF2352 at 147.898 kb on + strand, within GFF2352 at 147.898 kb on + strand, within GFF2352 at 147.922 kb on + strand, within GFF2352 at 147.922 kb on + strand, within GFF2352 at 147.922 kb on + strand, within GFF2352 at 147.979 kb on + strand, within GFF2352 at 147.979 kb on + strand, within GFF2352 at 147.980 kb on - strand, within GFF2352 at 148.056 kb on + strand, within GFF2352 at 148.056 kb on + strand, within GFF2352 at 148.057 kb on - strand, within GFF2352 at 148.076 kb on - strand, within GFF2352 at 148.101 kb on + strand, within GFF2352 at 148.128 kb on - strand, within GFF2352 at 148.200 kb on + strand, within GFF2352 at 148.201 kb on - strand, within GFF2352 at 148.232 kb on + strand, within GFF2352 at 148.271 kb on - strand, within GFF2352 at 148.297 kb on + strand, within GFF2352 at 148.303 kb on + strand, within GFF2352 at 148.307 kb on - strand, within GFF2352 at 148.411 kb on - strand, within GFF2352 at 148.411 kb on - strand, within GFF2352 at 148.413 kb on + strand, within GFF2352 at 148.413 kb on + strand, within GFF2352 at 148.524 kb on + strand, within GFF2352 at 148.524 kb on + strand, within GFF2352 at 148.534 kb on + strand, within GFF2352 at 148.569 kb on + strand, within GFF2352 at 148.606 kb on + strand, within GFF2352 at 148.651 kb on + strand, within GFF2352 at 148.651 kb on + strand, within GFF2352 at 148.651 kb on + strand, within GFF2352 at 148.652 kb on - strand, within GFF2352 at 148.657 kb on + strand, within GFF2352 at 148.693 kb on + strand, within GFF2352 at 148.730 kb on + strand at 148.740 kb on + strand at 148.787 kb on + strand at 149.692 kb on - strand at 149.867 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 146,072 + GFF2351 0.47 +0.1 146,120 - GFF2351 0.51 -0.0 146,160 - GFF2351 0.54 -0.4 146,160 - GFF2351 0.54 +0.7 146,285 + GFF2351 0.66 -0.0 146,290 + GFF2351 0.66 -0.1 146,332 + GFF2351 0.70 -0.3 146,352 - GFF2351 0.72 -1.4 146,405 + GFF2351 0.77 -0.3 146,474 + GFF2351 0.83 +0.9 146,474 + GFF2351 0.83 -0.7 146,474 + GFF2351 0.83 +0.6 146,475 - GFF2351 0.83 -0.3 146,482 + GFF2351 0.84 +0.4 146,526 - GFF2351 0.88 +0.0 146,526 - GFF2351 0.88 +1.2 146,554 - -0.4 146,588 + +0.2 146,589 - -0.7 146,629 + -0.5 146,629 + +0.2 146,630 - -0.3 146,630 - +1.1 146,630 - +0.5 146,630 - +0.5 146,630 - -0.1 146,723 - -0.4 146,805 + -0.6 146,805 + -0.5 146,806 - +1.5 146,806 - -0.2 146,871 - -0.3 146,871 - +0.3 146,985 - +0.4 147,015 - +0.2 147,015 - -0.2 147,016 + -0.4 147,016 + +0.6 147,320 + GFF2352 0.16 +0.3 147,320 + GFF2352 0.16 -0.8 147,321 - GFF2352 0.16 +0.7 147,321 - GFF2352 0.16 -1.5 147,418 + GFF2352 0.21 -0.7 147,418 + GFF2352 0.21 -0.7 147,418 + GFF2352 0.21 +0.7 147,477 + GFF2352 0.24 -0.5 147,483 + GFF2352 0.24 +2.2 147,534 + GFF2352 0.27 +0.1 147,534 + GFF2352 0.27 -1.3 147,691 - GFF2352 0.36 -0.4 147,701 - GFF2352 0.36 +1.6 147,717 + GFF2352 0.37 -1.1 147,788 - GFF2352 0.41 +1.8 147,874 - GFF2352 0.46 -0.1 147,874 - GFF2352 0.46 -0.7 147,898 + GFF2352 0.47 -0.0 147,898 + GFF2352 0.47 +0.9 147,898 + GFF2352 0.47 +0.5 147,922 + GFF2352 0.48 -0.1 147,922 + GFF2352 0.48 +0.9 147,922 + GFF2352 0.48 +1.3 147,979 + GFF2352 0.51 +1.0 147,979 + GFF2352 0.51 +0.3 147,980 - GFF2352 0.51 +0.5 148,056 + GFF2352 0.55 -0.4 148,056 + GFF2352 0.55 +0.2 148,057 - GFF2352 0.55 +4.1 148,076 - GFF2352 0.56 -0.1 148,101 + GFF2352 0.58 +1.9 148,128 - GFF2352 0.59 -0.0 148,200 + GFF2352 0.63 +0.6 148,201 - GFF2352 0.63 -0.1 148,232 + GFF2352 0.65 -1.1 148,271 - GFF2352 0.67 -0.1 148,297 + GFF2352 0.68 -0.2 148,303 + GFF2352 0.69 -0.4 148,307 - GFF2352 0.69 +0.7 148,411 - GFF2352 0.75 +0.4 148,411 - GFF2352 0.75 +0.1 148,413 + GFF2352 0.75 -0.6 148,413 + GFF2352 0.75 +2.2 148,524 + GFF2352 0.81 -0.2 148,524 + GFF2352 0.81 -0.2 148,534 + GFF2352 0.81 +0.6 148,569 + GFF2352 0.83 -1.2 148,606 + GFF2352 0.85 +4.9 148,651 + GFF2352 0.88 -0.9 148,651 + GFF2352 0.88 -0.5 148,651 + GFF2352 0.88 -3.1 148,652 - GFF2352 0.88 -0.2 148,657 + GFF2352 0.88 -0.1 148,693 + GFF2352 0.90 +0.3 148,730 + -0.2 148,740 + +0.3 148,787 + -1.0 149,692 - +0.1 149,867 + +1.8
Or see this region's nucleotide sequence