Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2335 and GFF2336 are separated by 14 nucleotides GFF2336 and GFF2337 are separated by 98 nucleotides
GFF2335 - PTS system, fructose-specific IIC component, at 126,225 to 127,304
GFF2335
GFF2336 - PTS system fructose-like IIB component 1 precursor (EC 2.7.1.69), at 127,319 to 127,639
GFF2336
GFF2337 - Pyruvate formate-lyase (EC 2.3.1.54), at 127,738 to 130,035
GFF2337
Position (kb)
127
128 Strain fitness (log2 ratio)
-2
-1
0
1 at 126.340 kb on + strand, within GFF2335 at 126.340 kb on + strand, within GFF2335 at 126.341 kb on - strand, within GFF2335 at 126.341 kb on - strand, within GFF2335 at 126.447 kb on + strand, within GFF2335 at 126.447 kb on + strand, within GFF2335 at 126.457 kb on - strand, within GFF2335 at 126.580 kb on - strand, within GFF2335 at 126.657 kb on - strand, within GFF2335 at 126.860 kb on - strand, within GFF2335 at 126.862 kb on - strand, within GFF2335 at 126.862 kb on - strand, within GFF2335 at 126.863 kb on + strand, within GFF2335 at 126.863 kb on + strand, within GFF2335 at 126.864 kb on - strand, within GFF2335 at 127.062 kb on - strand, within GFF2335 at 127.117 kb on - strand, within GFF2335 at 127.302 kb on + strand at 127.370 kb on + strand, within GFF2336 at 127.370 kb on + strand, within GFF2336 at 127.370 kb on + strand, within GFF2336 at 127.370 kb on + strand, within GFF2336 at 127.370 kb on + strand, within GFF2336 at 127.370 kb on + strand, within GFF2336 at 127.370 kb on + strand, within GFF2336 at 127.370 kb on + strand, within GFF2336 at 127.371 kb on - strand, within GFF2336 at 127.371 kb on - strand, within GFF2336 at 127.422 kb on + strand, within GFF2336 at 127.513 kb on + strand, within GFF2336 at 127.547 kb on - strand, within GFF2336 at 127.552 kb on + strand, within GFF2336 at 127.600 kb on + strand, within GFF2336 at 127.749 kb on + strand at 127.816 kb on + strand at 127.882 kb on + strand at 127.966 kb on - strand at 128.119 kb on - strand, within GFF2337 at 128.121 kb on + strand, within GFF2337 at 128.321 kb on + strand, within GFF2337 at 128.321 kb on + strand, within GFF2337 at 128.506 kb on - strand, within GFF2337 at 128.560 kb on + strand, within GFF2337 at 128.561 kb on - strand, within GFF2337 at 128.561 kb on - strand, within GFF2337 at 128.561 kb on - strand, within GFF2337
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 126,340 + GFF2335 0.11 -0.8 126,340 + GFF2335 0.11 +0.4 126,341 - GFF2335 0.11 +1.5 126,341 - GFF2335 0.11 -0.2 126,447 + GFF2335 0.21 +0.5 126,447 + GFF2335 0.21 +0.4 126,457 - GFF2335 0.21 +0.5 126,580 - GFF2335 0.33 -0.9 126,657 - GFF2335 0.40 -0.6 126,860 - GFF2335 0.59 +0.3 126,862 - GFF2335 0.59 -0.3 126,862 - GFF2335 0.59 -0.7 126,863 + GFF2335 0.59 -0.2 126,863 + GFF2335 0.59 +0.3 126,864 - GFF2335 0.59 +0.9 127,062 - GFF2335 0.78 +0.9 127,117 - GFF2335 0.83 +0.0 127,302 + -0.6 127,370 + GFF2336 0.16 -1.4 127,370 + GFF2336 0.16 -2.0 127,370 + GFF2336 0.16 -1.6 127,370 + GFF2336 0.16 -1.0 127,370 + GFF2336 0.16 -0.8 127,370 + GFF2336 0.16 -0.3 127,370 + GFF2336 0.16 -0.5 127,370 + GFF2336 0.16 -0.6 127,371 - GFF2336 0.16 -0.4 127,371 - GFF2336 0.16 +0.1 127,422 + GFF2336 0.32 -0.2 127,513 + GFF2336 0.60 +0.2 127,547 - GFF2336 0.71 +0.3 127,552 + GFF2336 0.73 -0.1 127,600 + GFF2336 0.88 -1.2 127,749 + +0.5 127,816 + +0.2 127,882 + -1.9 127,966 - -1.9 128,119 - GFF2337 0.17 +0.3 128,121 + GFF2337 0.17 -0.7 128,321 + GFF2337 0.25 -0.1 128,321 + GFF2337 0.25 +0.1 128,506 - GFF2337 0.33 +0.3 128,560 + GFF2337 0.36 -1.4 128,561 - GFF2337 0.36 -0.1 128,561 - GFF2337 0.36 -0.2 128,561 - GFF2337 0.36 +0.5
Or see this region's nucleotide sequence