Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2294 and GFF2295 are separated by 255 nucleotides GFF2295 and GFF2296 overlap by 7 nucleotides
GFF2294 - LsrR, transcriptional repressor of lsr operon, at 81,210 to 82,169
GFF2294
GFF2295 - Autoinducer 2 (AI-2) ABC transport system, fused AI2 transporter subunits and ATP-binding component, at 82,425 to 83,960
GFF2295
GFF2296 - Autoinducer 2 (AI-2) ABC transport system, membrane channel protein LsrC, at 83,954 to 84,997
GFF2296
Position (kb) 82
83
84 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 81.507 kb on + strand, within GFF2294 at 81.507 kb on + strand, within GFF2294 at 81.659 kb on + strand, within GFF2294 at 81.659 kb on + strand, within GFF2294 at 81.660 kb on - strand, within GFF2294 at 81.662 kb on + strand, within GFF2294 at 81.662 kb on + strand, within GFF2294 at 81.666 kb on + strand, within GFF2294 at 81.666 kb on + strand, within GFF2294 at 81.667 kb on - strand, within GFF2294 at 81.672 kb on - strand, within GFF2294 at 81.705 kb on - strand, within GFF2294 at 81.707 kb on - strand, within GFF2294 at 81.707 kb on - strand, within GFF2294 at 81.707 kb on - strand, within GFF2294 at 81.848 kb on + strand, within GFF2294 at 82.096 kb on + strand at 82.097 kb on - strand at 82.109 kb on + strand at 82.157 kb on + strand at 82.442 kb on + strand at 82.477 kb on - strand at 82.520 kb on + strand at 82.583 kb on - strand, within GFF2295 at 82.658 kb on - strand, within GFF2295 at 82.727 kb on - strand, within GFF2295 at 82.743 kb on - strand, within GFF2295 at 82.795 kb on + strand, within GFF2295 at 82.909 kb on + strand, within GFF2295 at 82.909 kb on + strand, within GFF2295 at 83.129 kb on + strand, within GFF2295 at 83.130 kb on - strand, within GFF2295 at 83.227 kb on + strand, within GFF2295 at 83.230 kb on + strand, within GFF2295 at 83.267 kb on + strand, within GFF2295 at 83.268 kb on - strand, within GFF2295 at 83.449 kb on - strand, within GFF2295 at 83.821 kb on + strand at 83.822 kb on - strand at 83.842 kb on + strand at 83.842 kb on + strand at 83.842 kb on + strand at 83.843 kb on - strand at 83.843 kb on - strand at 83.844 kb on + strand at 83.844 kb on + strand at 83.844 kb on + strand at 83.845 kb on - strand at 83.845 kb on - strand at 83.897 kb on + strand at 83.906 kb on + strand at 83.906 kb on + strand at 84.013 kb on - strand at 84.044 kb on + strand at 84.147 kb on + strand, within GFF2296 at 84.209 kb on + strand, within GFF2296 at 84.241 kb on - strand, within GFF2296 at 84.243 kb on + strand, within GFF2296 at 84.244 kb on - strand, within GFF2296 at 84.355 kb on - strand, within GFF2296 at 84.469 kb on + strand, within GFF2296 at 84.841 kb on - strand, within GFF2296 at 84.922 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 81,507 + GFF2294 0.31 +1.6 81,507 + GFF2294 0.31 -1.2 81,659 + GFF2294 0.47 -0.4 81,659 + GFF2294 0.47 -0.9 81,660 - GFF2294 0.47 -0.0 81,662 + GFF2294 0.47 -0.4 81,662 + GFF2294 0.47 -0.5 81,666 + GFF2294 0.47 +0.1 81,666 + GFF2294 0.47 +0.3 81,667 - GFF2294 0.48 -0.5 81,672 - GFF2294 0.48 -0.5 81,705 - GFF2294 0.52 -0.9 81,707 - GFF2294 0.52 -0.4 81,707 - GFF2294 0.52 +0.6 81,707 - GFF2294 0.52 -0.9 81,848 + GFF2294 0.66 -0.9 82,096 + +0.6 82,097 - -0.9 82,109 + -0.4 82,157 + -0.0 82,442 + +0.1 82,477 - -0.1 82,520 + +1.9 82,583 - GFF2295 0.10 -1.7 82,658 - GFF2295 0.15 -0.6 82,727 - GFF2295 0.20 -0.2 82,743 - GFF2295 0.21 -0.3 82,795 + GFF2295 0.24 -0.0 82,909 + GFF2295 0.32 -0.3 82,909 + GFF2295 0.32 +1.7 83,129 + GFF2295 0.46 -1.2 83,130 - GFF2295 0.46 +3.8 83,227 + GFF2295 0.52 -1.7 83,230 + GFF2295 0.52 -0.1 83,267 + GFF2295 0.55 +0.1 83,268 - GFF2295 0.55 -0.9 83,449 - GFF2295 0.67 -0.1 83,821 + +0.6 83,822 - -0.3 83,842 + -2.2 83,842 + -0.1 83,842 + -0.4 83,843 - +0.7 83,843 - +0.9 83,844 + -0.6 83,844 + -0.9 83,844 + -0.4 83,845 - +0.2 83,845 - -0.4 83,897 + +0.3 83,906 + +0.6 83,906 + +1.3 84,013 - +0.7 84,044 + -0.2 84,147 + GFF2296 0.18 +2.4 84,209 + GFF2296 0.24 +0.6 84,241 - GFF2296 0.27 -0.1 84,243 + GFF2296 0.28 +2.6 84,244 - GFF2296 0.28 +0.3 84,355 - GFF2296 0.38 +0.5 84,469 + GFF2296 0.49 +0.0 84,841 - GFF2296 0.85 +0.8 84,922 + +0.2
Or see this region's nucleotide sequence