Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2129 and GFF2130 are separated by 392 nucleotides GFF2130 and GFF2131 are separated by 0 nucleotides GFF2131 and GFF2132 are separated by 112 nucleotides
GFF2129 - Biotin carboxyl carrier protein of acetyl-CoA carboxylase, at 16,354 to 16,824
GFF2129
GFF2130 - FIG001196: Membrane protein YedZ, at 17,217 to 17,816
GFF2130
GFF2131 - Putative sulfite oxidase subunit YedY, at 17,817 to 18,821
GFF2131
GFF2132 - Alcohol dehydrogenase (EC 1.1.1.1), at 18,934 to 19,908
GFF2132
Position (kb) 17
18
19 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 17.082 kb on + strand at 17.096 kb on - strand at 17.101 kb on + strand at 17.102 kb on - strand at 17.271 kb on + strand at 17.272 kb on - strand at 17.282 kb on - strand, within GFF2130 at 17.323 kb on + strand, within GFF2130 at 17.324 kb on - strand, within GFF2130 at 17.328 kb on + strand, within GFF2130 at 17.329 kb on - strand, within GFF2130 at 17.353 kb on + strand, within GFF2130 at 17.448 kb on - strand, within GFF2130 at 17.508 kb on - strand, within GFF2130 at 17.534 kb on - strand, within GFF2130 at 17.534 kb on - strand, within GFF2130 at 17.534 kb on - strand, within GFF2130 at 17.542 kb on - strand, within GFF2130 at 17.601 kb on + strand, within GFF2130 at 17.989 kb on + strand, within GFF2131 at 17.999 kb on - strand, within GFF2131 at 18.071 kb on + strand, within GFF2131 at 18.080 kb on + strand, within GFF2131 at 18.080 kb on + strand, within GFF2131 at 18.088 kb on + strand, within GFF2131 at 18.143 kb on + strand, within GFF2131 at 18.144 kb on - strand, within GFF2131 at 18.148 kb on + strand, within GFF2131 at 18.217 kb on + strand, within GFF2131 at 18.260 kb on + strand, within GFF2131 at 18.261 kb on - strand, within GFF2131 at 18.261 kb on - strand, within GFF2131 at 18.300 kb on - strand, within GFF2131 at 18.331 kb on - strand, within GFF2131 at 18.335 kb on + strand, within GFF2131 at 18.335 kb on + strand, within GFF2131 at 18.336 kb on - strand, within GFF2131 at 18.336 kb on - strand, within GFF2131 at 18.336 kb on - strand, within GFF2131 at 18.336 kb on - strand, within GFF2131 at 18.336 kb on - strand, within GFF2131 at 18.336 kb on - strand, within GFF2131 at 18.338 kb on - strand, within GFF2131 at 18.339 kb on + strand, within GFF2131 at 18.339 kb on - strand, within GFF2131 at 18.340 kb on - strand, within GFF2131 at 18.402 kb on - strand, within GFF2131 at 18.402 kb on - strand, within GFF2131 at 18.411 kb on + strand, within GFF2131 at 18.430 kb on - strand, within GFF2131 at 18.450 kb on - strand, within GFF2131 at 18.450 kb on - strand, within GFF2131 at 18.534 kb on - strand, within GFF2131 at 18.563 kb on - strand, within GFF2131 at 18.575 kb on + strand, within GFF2131 at 18.715 kb on - strand, within GFF2131 at 18.736 kb on - strand at 18.810 kb on + strand at 18.811 kb on - strand at 19.015 kb on - strand at 19.037 kb on + strand, within GFF2132 at 19.037 kb on + strand, within GFF2132 at 19.038 kb on - strand, within GFF2132 at 19.038 kb on - strand, within GFF2132 at 19.114 kb on + strand, within GFF2132 at 19.115 kb on - strand, within GFF2132 at 19.134 kb on + strand, within GFF2132 at 19.153 kb on - strand, within GFF2132 at 19.211 kb on + strand, within GFF2132 at 19.211 kb on + strand, within GFF2132 at 19.276 kb on + strand, within GFF2132 at 19.348 kb on - strand, within GFF2132 at 19.405 kb on - strand, within GFF2132 at 19.658 kb on - strand, within GFF2132 at 19.660 kb on + strand, within GFF2132 at 19.660 kb on + strand, within GFF2132 at 19.770 kb on + strand, within GFF2132 at 19.786 kb on - strand, within GFF2132
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 17,082 + -0.9 17,096 - -0.6 17,101 + -0.8 17,102 - -0.6 17,271 + -0.7 17,272 - +0.2 17,282 - GFF2130 0.11 -0.3 17,323 + GFF2130 0.18 -0.1 17,324 - GFF2130 0.18 +0.8 17,328 + GFF2130 0.18 -0.0 17,329 - GFF2130 0.19 +0.1 17,353 + GFF2130 0.23 -0.9 17,448 - GFF2130 0.39 -0.8 17,508 - GFF2130 0.48 -0.6 17,534 - GFF2130 0.53 +2.8 17,534 - GFF2130 0.53 -1.4 17,534 - GFF2130 0.53 +0.7 17,542 - GFF2130 0.54 -0.1 17,601 + GFF2130 0.64 +0.2 17,989 + GFF2131 0.17 -0.1 17,999 - GFF2131 0.18 -0.0 18,071 + GFF2131 0.25 -1.3 18,080 + GFF2131 0.26 -0.7 18,080 + GFF2131 0.26 -0.4 18,088 + GFF2131 0.27 +3.0 18,143 + GFF2131 0.32 -0.8 18,144 - GFF2131 0.33 -1.0 18,148 + GFF2131 0.33 +0.8 18,217 + GFF2131 0.40 +0.7 18,260 + GFF2131 0.44 +0.2 18,261 - GFF2131 0.44 -0.1 18,261 - GFF2131 0.44 -0.3 18,300 - GFF2131 0.48 -0.6 18,331 - GFF2131 0.51 +1.1 18,335 + GFF2131 0.52 +0.4 18,335 + GFF2131 0.52 +0.5 18,336 - GFF2131 0.52 +0.7 18,336 - GFF2131 0.52 +1.5 18,336 - GFF2131 0.52 +0.0 18,336 - GFF2131 0.52 +0.7 18,336 - GFF2131 0.52 +0.4 18,336 - GFF2131 0.52 -0.2 18,338 - GFF2131 0.52 -0.2 18,339 + GFF2131 0.52 -0.9 18,339 - GFF2131 0.52 +1.6 18,340 - GFF2131 0.52 -0.5 18,402 - GFF2131 0.58 +0.9 18,402 - GFF2131 0.58 -0.4 18,411 + GFF2131 0.59 -0.1 18,430 - GFF2131 0.61 -0.1 18,450 - GFF2131 0.63 -0.2 18,450 - GFF2131 0.63 -0.1 18,534 - GFF2131 0.71 +1.8 18,563 - GFF2131 0.74 +4.2 18,575 + GFF2131 0.75 -0.0 18,715 - GFF2131 0.89 +1.1 18,736 - -0.3 18,810 + +1.7 18,811 - +0.9 19,015 - -0.2 19,037 + GFF2132 0.11 -0.2 19,037 + GFF2132 0.11 +0.2 19,038 - GFF2132 0.11 +0.1 19,038 - GFF2132 0.11 -0.6 19,114 + GFF2132 0.18 -0.9 19,115 - GFF2132 0.19 +1.5 19,134 + GFF2132 0.21 -1.3 19,153 - GFF2132 0.22 -0.3 19,211 + GFF2132 0.28 +1.3 19,211 + GFF2132 0.28 +0.4 19,276 + GFF2132 0.35 +4.4 19,348 - GFF2132 0.42 +1.7 19,405 - GFF2132 0.48 +1.6 19,658 - GFF2132 0.74 -0.3 19,660 + GFF2132 0.74 -0.5 19,660 + GFF2132 0.74 +0.5 19,770 + GFF2132 0.86 -0.3 19,786 - GFF2132 0.87 -0.1
Or see this region's nucleotide sequence