Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2071 and GFF2072 are separated by 9 nucleotides GFF2072 and GFF2073 are separated by 18 nucleotides GFF2073 and GFF2074 are separated by 11 nucleotides
GFF2071 - Ethanolamine utilization polyhedral-body-like protein EutL, at 33,686 to 34,345
GFF2071
GFF2072 - Ethanolamine ammonia-lyase light chain (EC 4.3.1.7), at 34,355 to 35,251
GFF2072
GFF2073 - Ethanolamine ammonia-lyase heavy chain (EC 4.3.1.7), at 35,270 to 36,631
GFF2073
GFF2074 - Ethanolamine utilization protein EutA, at 36,643 to 38,046
GFF2074
Position (kb) 35
36
37 Strain fitness (log2 ratio) -1
0
1
2
3 at 34.710 kb on + strand, within GFF2072 at 34.710 kb on + strand, within GFF2072 at 34.711 kb on - strand, within GFF2072 at 34.754 kb on + strand, within GFF2072 at 34.754 kb on + strand, within GFF2072 at 34.846 kb on - strand, within GFF2072 at 35.222 kb on + strand at 35.421 kb on + strand, within GFF2073 at 35.422 kb on - strand, within GFF2073 at 35.464 kb on - strand, within GFF2073 at 35.464 kb on - strand, within GFF2073 at 35.581 kb on + strand, within GFF2073 at 35.626 kb on - strand, within GFF2073 at 35.626 kb on - strand, within GFF2073 at 35.701 kb on + strand, within GFF2073 at 35.701 kb on + strand, within GFF2073 at 35.702 kb on - strand, within GFF2073 at 35.778 kb on + strand, within GFF2073 at 35.779 kb on - strand, within GFF2073 at 36.115 kb on + strand, within GFF2073 at 36.122 kb on + strand, within GFF2073 at 36.336 kb on + strand, within GFF2073 at 36.590 kb on - strand at 36.719 kb on + strand at 36.720 kb on - strand at 36.720 kb on - strand at 36.720 kb on - strand at 36.773 kb on - strand at 36.787 kb on + strand, within GFF2074 at 36.788 kb on - strand, within GFF2074 at 36.788 kb on - strand, within GFF2074 at 37.069 kb on - strand, within GFF2074 at 37.072 kb on - strand, within GFF2074 at 37.092 kb on + strand, within GFF2074 at 37.242 kb on - strand, within GFF2074 at 37.285 kb on - strand, within GFF2074 at 37.285 kb on - strand, within GFF2074 at 37.569 kb on - strand, within GFF2074
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 34,710 + GFF2072 0.40 +2.4 34,710 + GFF2072 0.40 +0.2 34,711 - GFF2072 0.40 -0.3 34,754 + GFF2072 0.44 -1.7 34,754 + GFF2072 0.44 -0.7 34,846 - GFF2072 0.55 +2.1 35,222 + -0.5 35,421 + GFF2073 0.11 -0.2 35,422 - GFF2073 0.11 +1.0 35,464 - GFF2073 0.14 +0.4 35,464 - GFF2073 0.14 +0.6 35,581 + GFF2073 0.23 +0.2 35,626 - GFF2073 0.26 +0.2 35,626 - GFF2073 0.26 +1.6 35,701 + GFF2073 0.32 -0.7 35,701 + GFF2073 0.32 -0.7 35,702 - GFF2073 0.32 +0.1 35,778 + GFF2073 0.37 +1.9 35,779 - GFF2073 0.37 +0.2 36,115 + GFF2073 0.62 -1.7 36,122 + GFF2073 0.63 -0.0 36,336 + GFF2073 0.78 +0.3 36,590 - -0.0 36,719 + +2.0 36,720 - -1.4 36,720 - +0.7 36,720 - -0.5 36,773 - +0.2 36,787 + GFF2074 0.10 +0.4 36,788 - GFF2074 0.10 +3.7 36,788 - GFF2074 0.10 +0.2 37,069 - GFF2074 0.30 +1.6 37,072 - GFF2074 0.31 -0.4 37,092 + GFF2074 0.32 -1.1 37,242 - GFF2074 0.43 +1.0 37,285 - GFF2074 0.46 -0.7 37,285 - GFF2074 0.46 -0.9 37,569 - GFF2074 0.66 +0.2
Or see this region's nucleotide sequence