Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2068 and GFF2069 are separated by 199 nucleotides GFF2069 and GFF2070 are separated by 47 nucleotides GFF2070 and GFF2071 are separated by 12 nucleotides
GFF2068 - FIG01045300: hypothetical protein, at 30,602 to 31,879
GFF2068
GFF2069 - Ethanolamine operon regulatory protein, at 32,079 to 33,131
GFF2069
GFF2070 - Ethanolamine utilization polyhedral-body-like protein EutK, at 33,179 to 33,673
GFF2070
GFF2071 - Ethanolamine utilization polyhedral-body-like protein EutL, at 33,686 to 34,345
GFF2071
Position (kb) 32
33
34 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 31.370 kb on + strand, within GFF2068 at 31.504 kb on + strand, within GFF2068 at 31.733 kb on - strand, within GFF2068 at 31.777 kb on - strand at 31.782 kb on - strand at 31.831 kb on + strand at 32.133 kb on + strand at 32.133 kb on + strand at 32.226 kb on - strand, within GFF2069 at 32.259 kb on - strand, within GFF2069 at 32.328 kb on + strand, within GFF2069 at 32.328 kb on + strand, within GFF2069 at 32.329 kb on - strand, within GFF2069 at 32.346 kb on + strand, within GFF2069 at 32.347 kb on - strand, within GFF2069 at 32.414 kb on + strand, within GFF2069 at 32.447 kb on + strand, within GFF2069 at 32.494 kb on - strand, within GFF2069 at 32.494 kb on - strand, within GFF2069 at 32.514 kb on - strand, within GFF2069 at 32.514 kb on - strand, within GFF2069 at 32.639 kb on + strand, within GFF2069 at 32.640 kb on - strand, within GFF2069 at 32.640 kb on - strand, within GFF2069 at 32.688 kb on - strand, within GFF2069 at 32.722 kb on + strand, within GFF2069 at 33.095 kb on - strand at 33.116 kb on + strand at 33.607 kb on + strand, within GFF2070 at 33.707 kb on - strand at 33.733 kb on + strand at 33.819 kb on + strand, within GFF2071 at 33.942 kb on + strand, within GFF2071 at 33.943 kb on - strand, within GFF2071
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 31,370 + GFF2068 0.60 +0.1 31,504 + GFF2068 0.71 -0.1 31,733 - GFF2068 0.88 -0.0 31,777 - -0.6 31,782 - -0.9 31,831 + -0.6 32,133 + +1.3 32,133 + +0.4 32,226 - GFF2069 0.14 -0.1 32,259 - GFF2069 0.17 -0.4 32,328 + GFF2069 0.24 +2.5 32,328 + GFF2069 0.24 -0.3 32,329 - GFF2069 0.24 +1.0 32,346 + GFF2069 0.25 -0.3 32,347 - GFF2069 0.25 +0.7 32,414 + GFF2069 0.32 -0.3 32,447 + GFF2069 0.35 -0.8 32,494 - GFF2069 0.39 -2.0 32,494 - GFF2069 0.39 -0.2 32,514 - GFF2069 0.41 +0.7 32,514 - GFF2069 0.41 -1.3 32,639 + GFF2069 0.53 -0.2 32,640 - GFF2069 0.53 +0.3 32,640 - GFF2069 0.53 +0.9 32,688 - GFF2069 0.58 -0.0 32,722 + GFF2069 0.61 +2.3 33,095 - -0.0 33,116 + +0.1 33,607 + GFF2070 0.86 +2.8 33,707 - +3.1 33,733 + +0.2 33,819 + GFF2071 0.20 +1.0 33,942 + GFF2071 0.39 +2.4 33,943 - GFF2071 0.39 +1.1
Or see this region's nucleotide sequence