Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2065 and GFF2066 are separated by 2 nucleotides GFF2066 and GFF2067 are separated by 161 nucleotides GFF2067 and GFF2068 are separated by 128 nucleotides
GFF2065 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), at 28,352 to 29,221
GFF2065
GFF2066 - Coproporphyrinogen III oxidase, aerobic (EC 1.3.3.3), at 29,224 to 30,123
GFF2066
GFF2067 - Putative membrane protein, at 30,285 to 30,473
GFF2067
GFF2068 - FIG01045300: hypothetical protein, at 30,602 to 31,879
GFF2068
Position (kb)
29
30
31 Strain fitness (log2 ratio)
-1
0
1
2 at 28.267 kb on + strand at 28.317 kb on - strand at 28.436 kb on - strand at 28.436 kb on - strand at 28.450 kb on - strand, within GFF2065 at 28.718 kb on - strand, within GFF2065 at 28.739 kb on - strand, within GFF2065 at 28.835 kb on + strand, within GFF2065 at 28.836 kb on - strand, within GFF2065 at 28.905 kb on + strand, within GFF2065 at 28.906 kb on - strand, within GFF2065 at 29.086 kb on + strand, within GFF2065 at 29.370 kb on - strand, within GFF2066 at 29.525 kb on - strand, within GFF2066 at 29.525 kb on - strand, within GFF2066 at 29.623 kb on + strand, within GFF2066 at 29.624 kb on - strand, within GFF2066 at 29.848 kb on + strand, within GFF2066 at 30.100 kb on - strand at 30.132 kb on + strand at 30.132 kb on + strand at 30.137 kb on + strand at 30.169 kb on + strand at 30.245 kb on - strand at 30.358 kb on + strand, within GFF2067 at 30.369 kb on - strand, within GFF2067 at 30.415 kb on + strand, within GFF2067 at 30.551 kb on - strand at 30.561 kb on + strand at 30.566 kb on + strand at 30.685 kb on + strand at 30.686 kb on - strand at 30.728 kb on + strand at 30.729 kb on - strand at 30.773 kb on - strand, within GFF2068 at 30.869 kb on + strand, within GFF2068 at 30.911 kb on + strand, within GFF2068 at 31.021 kb on - strand, within GFF2068
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 28,267 + -0.2 28,317 - -0.8 28,436 - +0.3 28,436 - +0.7 28,450 - GFF2065 0.11 -1.2 28,718 - GFF2065 0.42 +0.2 28,739 - GFF2065 0.44 +0.3 28,835 + GFF2065 0.56 -0.3 28,836 - GFF2065 0.56 -0.4 28,905 + GFF2065 0.64 +0.8 28,906 - GFF2065 0.64 +0.8 29,086 + GFF2065 0.84 -0.3 29,370 - GFF2066 0.16 +0.1 29,525 - GFF2066 0.33 +0.1 29,525 - GFF2066 0.33 +1.3 29,623 + GFF2066 0.44 +1.0 29,624 - GFF2066 0.44 +0.9 29,848 + GFF2066 0.69 +2.6 30,100 - -0.1 30,132 + -0.0 30,132 + +1.2 30,137 + +0.3 30,169 + +2.5 30,245 - +0.5 30,358 + GFF2067 0.39 +0.9 30,369 - GFF2067 0.44 -0.5 30,415 + GFF2067 0.69 +1.8 30,551 - -0.5 30,561 + +0.7 30,566 + +1.7 30,685 + -0.1 30,686 - +1.6 30,728 + +0.3 30,729 - +0.9 30,773 - GFF2068 0.13 -1.5 30,869 + GFF2068 0.21 -0.0 30,911 + GFF2068 0.24 +0.0 31,021 - GFF2068 0.33 +1.1
Or see this region's nucleotide sequence