Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2024 and GFF2025 are separated by 247 nucleotides GFF2025 and GFF2026 overlap by 4 nucleotides GFF2026 and GFF2027 are separated by 394 nucleotides
GFF2024 - Nonspecific acid phosphatase precursor (EC 3.1.3.2), at 32,133 to 32,885
GFF2024
GFF2025 - FIG001353: Acetyltransferase, at 33,133 to 33,624
GFF2025
GFF2026 - FIG032766: hypothetical protein, at 33,621 to 33,914
GFF2026
GFF2027 - putative cytoplasmic protein, at 34,309 to 34,452
GFF2027
Position (kb) 33
34 Strain fitness (log2 ratio) -2
-1
0
1
2 at 32.452 kb on + strand, within GFF2024 at 32.570 kb on + strand, within GFF2024 at 32.571 kb on - strand, within GFF2024 at 32.613 kb on + strand, within GFF2024 at 32.618 kb on + strand, within GFF2024 at 32.764 kb on + strand, within GFF2024 at 32.848 kb on + strand at 33.084 kb on - strand at 33.114 kb on - strand at 33.197 kb on - strand, within GFF2025 at 33.270 kb on + strand, within GFF2025 at 33.396 kb on + strand, within GFF2025 at 33.456 kb on - strand, within GFF2025 at 33.467 kb on - strand, within GFF2025 at 33.550 kb on - strand, within GFF2025 at 33.553 kb on + strand, within GFF2025 at 33.554 kb on - strand, within GFF2025 at 33.582 kb on + strand at 33.583 kb on - strand at 33.583 kb on - strand at 33.914 kb on + strand at 33.931 kb on - strand at 33.931 kb on - strand at 33.946 kb on - strand at 33.951 kb on + strand at 33.951 kb on + strand at 34.115 kb on + strand at 34.141 kb on + strand at 34.165 kb on - strand at 34.293 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 32,452 + GFF2024 0.42 -0.3 32,570 + GFF2024 0.58 +0.6 32,571 - GFF2024 0.58 -0.6 32,613 + GFF2024 0.64 +0.1 32,618 + GFF2024 0.64 +0.6 32,764 + GFF2024 0.84 -0.2 32,848 + -0.1 33,084 - -0.1 33,114 - -2.2 33,197 - GFF2025 0.13 +1.0 33,270 + GFF2025 0.28 -0.3 33,396 + GFF2025 0.53 +1.3 33,456 - GFF2025 0.66 -0.3 33,467 - GFF2025 0.68 +2.2 33,550 - GFF2025 0.85 -0.4 33,553 + GFF2025 0.85 +0.1 33,554 - GFF2025 0.86 -0.3 33,582 + -0.4 33,583 - +0.6 33,583 - +0.2 33,914 + +0.6 33,931 - -0.2 33,931 - +0.7 33,946 - -0.4 33,951 + +0.4 33,951 + +1.7 34,115 + -0.0 34,141 + -0.3 34,165 - +0.5 34,293 - -0.1
Or see this region's nucleotide sequence