Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2013 and GFF2014 are separated by 114 nucleotides GFF2014 and GFF2015 are separated by 120 nucleotides GFF2015 and GFF2016 overlap by 25 nucleotides
GFF2013 - Aspartate ammonia-lyase (EC 4.3.1.1), at 24,093 to 25,529
GFF2013
GFF2014 - C4-dicarboxylate transporter DcuA, at 25,644 to 26,945
GFF2014
GFF2015 - Periplasmic divalent cation tolerance protein CutA, at 27,066 to 27,413
GFF2015
GFF2016 - Cytochrome c-type biogenesis protein DsbD, protein-disulfide reductase (EC 1.8.1.8), at 27,389 to 29,092
GFF2016
Position (kb) 25
26
27 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 24.825 kb on + strand, within GFF2013 at 24.921 kb on + strand, within GFF2013 at 24.921 kb on + strand, within GFF2013 at 24.922 kb on - strand, within GFF2013 at 24.922 kb on - strand, within GFF2013 at 24.922 kb on - strand, within GFF2013 at 25.108 kb on + strand, within GFF2013 at 25.116 kb on - strand, within GFF2013 at 25.243 kb on - strand, within GFF2013 at 25.308 kb on + strand, within GFF2013 at 25.309 kb on - strand, within GFF2013 at 25.309 kb on - strand, within GFF2013 at 25.311 kb on - strand, within GFF2013 at 25.457 kb on + strand at 25.492 kb on - strand at 25.493 kb on + strand at 25.494 kb on - strand at 25.499 kb on + strand at 25.506 kb on + strand at 25.509 kb on - strand at 25.541 kb on - strand at 25.544 kb on - strand at 25.582 kb on - strand at 25.643 kb on + strand at 25.648 kb on + strand at 25.648 kb on + strand at 25.649 kb on - strand at 25.830 kb on - strand, within GFF2014 at 25.886 kb on + strand, within GFF2014 at 25.899 kb on + strand, within GFF2014 at 25.899 kb on + strand, within GFF2014 at 26.097 kb on + strand, within GFF2014 at 26.099 kb on + strand, within GFF2014 at 26.100 kb on - strand, within GFF2014 at 26.129 kb on + strand, within GFF2014 at 26.130 kb on - strand, within GFF2014 at 26.134 kb on - strand, within GFF2014 at 26.134 kb on - strand, within GFF2014 at 26.135 kb on + strand, within GFF2014 at 26.136 kb on - strand, within GFF2014 at 26.345 kb on + strand, within GFF2014 at 26.534 kb on + strand, within GFF2014 at 26.534 kb on + strand, within GFF2014 at 26.535 kb on - strand, within GFF2014 at 26.535 kb on - strand, within GFF2014 at 26.540 kb on + strand, within GFF2014 at 26.540 kb on + strand, within GFF2014 at 26.541 kb on - strand, within GFF2014 at 26.676 kb on + strand, within GFF2014 at 26.793 kb on + strand, within GFF2014 at 26.816 kb on - strand at 26.936 kb on + strand at 26.937 kb on - strand at 26.937 kb on - strand at 26.944 kb on - strand at 26.966 kb on - strand at 27.019 kb on + strand at 27.020 kb on - strand at 27.020 kb on - strand at 27.021 kb on + strand at 27.065 kb on + strand at 27.223 kb on - strand, within GFF2015 at 27.252 kb on + strand, within GFF2015 at 27.253 kb on - strand, within GFF2015 at 27.375 kb on - strand, within GFF2015 at 27.381 kb on + strand at 27.382 kb on - strand at 27.382 kb on - strand at 27.407 kb on - strand at 27.563 kb on + strand, within GFF2016 at 27.564 kb on - strand, within GFF2016 at 27.564 kb on - strand, within GFF2016 at 27.699 kb on + strand, within GFF2016 at 27.743 kb on + strand, within GFF2016 at 27.744 kb on - strand, within GFF2016 at 27.744 kb on - strand, within GFF2016 at 27.886 kb on - strand, within GFF2016 at 27.922 kb on - strand, within GFF2016
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 24,825 + GFF2013 0.51 -0.8 24,921 + GFF2013 0.58 +0.2 24,921 + GFF2013 0.58 +0.8 24,922 - GFF2013 0.58 +0.5 24,922 - GFF2013 0.58 +0.7 24,922 - GFF2013 0.58 +1.4 25,108 + GFF2013 0.71 +0.7 25,116 - GFF2013 0.71 -0.2 25,243 - GFF2013 0.80 -0.1 25,308 + GFF2013 0.85 +4.5 25,309 - GFF2013 0.85 +2.0 25,309 - GFF2013 0.85 -0.8 25,311 - GFF2013 0.85 -0.4 25,457 + -0.1 25,492 - +1.3 25,493 + -0.1 25,494 - -1.4 25,499 + -0.2 25,506 + -0.2 25,509 - -0.0 25,541 - -0.0 25,544 - +0.8 25,582 - +3.4 25,643 + +1.1 25,648 + +0.7 25,648 + +0.6 25,649 - +0.2 25,830 - GFF2014 0.14 -0.3 25,886 + GFF2014 0.19 +3.3 25,899 + GFF2014 0.20 -0.2 25,899 + GFF2014 0.20 +0.5 26,097 + GFF2014 0.35 -0.4 26,099 + GFF2014 0.35 -0.1 26,100 - GFF2014 0.35 -0.3 26,129 + GFF2014 0.37 +1.1 26,130 - GFF2014 0.37 -0.6 26,134 - GFF2014 0.38 -0.0 26,134 - GFF2014 0.38 +1.2 26,135 + GFF2014 0.38 +0.1 26,136 - GFF2014 0.38 +0.0 26,345 + GFF2014 0.54 -0.2 26,534 + GFF2014 0.68 -0.9 26,534 + GFF2014 0.68 -0.6 26,535 - GFF2014 0.68 -0.7 26,535 - GFF2014 0.68 +0.8 26,540 + GFF2014 0.69 -0.3 26,540 + GFF2014 0.69 +2.7 26,541 - GFF2014 0.69 -0.2 26,676 + GFF2014 0.79 +0.5 26,793 + GFF2014 0.88 -0.2 26,816 - +0.3 26,936 + +0.5 26,937 - +0.2 26,937 - +0.1 26,944 - -0.1 26,966 - +1.2 27,019 + +0.2 27,020 - +0.0 27,020 - -0.8 27,021 + +0.1 27,065 + -0.1 27,223 - GFF2015 0.45 +0.2 27,252 + GFF2015 0.53 -0.2 27,253 - GFF2015 0.54 -0.0 27,375 - GFF2015 0.89 -0.3 27,381 + -0.5 27,382 - +0.6 27,382 - -0.3 27,407 - +1.4 27,563 + GFF2016 0.10 -0.2 27,564 - GFF2016 0.10 +0.5 27,564 - GFF2016 0.10 -0.7 27,699 + GFF2016 0.18 +0.0 27,743 + GFF2016 0.21 -0.2 27,744 - GFF2016 0.21 +0.0 27,744 - GFF2016 0.21 -0.8 27,886 - GFF2016 0.29 -0.8 27,922 - GFF2016 0.31 -1.0
Or see this region's nucleotide sequence