Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1909 and GFF1910 are separated by 5 nucleotides GFF1910 and GFF1911 are separated by 56 nucleotides GFF1911 and GFF1912 are separated by 55 nucleotides GFF1912 and GFF1913 are separated by 142 nucleotides
GFF1909 - Transcriptional regulator, LacI family, at 103,773 to 104,792
GFF1909
GFF1910 - Putative Nudix hydrolase YfcD (EC 3.6.-.-), at 104,798 to 105,352
GFF1910
GFF1911 - FIG002292: Phosphodiesterase yfcE (EC 3.1.4.-), at 105,409 to 105,960
GFF1911
GFF1912 - Probable glutathione S-transferase (EC 2.5.1.18), YfcF homolog, at 106,016 to 106,660
GFF1912
GFF1913 - Probable glutathione S-transferase (EC 2.5.1.18), YfcG homolog, at 106,803 to 107,450
GFF1913
Position (kb)
105
106 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 104.423 kb on + strand, within GFF1909 at 104.423 kb on + strand, within GFF1909 at 104.423 kb on + strand, within GFF1909 at 104.424 kb on - strand, within GFF1909 at 104.424 kb on - strand, within GFF1909 at 104.444 kb on + strand, within GFF1909 at 104.531 kb on - strand, within GFF1909 at 104.563 kb on + strand, within GFF1909 at 104.770 kb on + strand at 104.770 kb on + strand at 104.770 kb on + strand at 104.770 kb on + strand at 104.771 kb on - strand at 105.067 kb on + strand, within GFF1910 at 105.149 kb on + strand, within GFF1910 at 105.150 kb on - strand, within GFF1910 at 105.150 kb on - strand, within GFF1910 at 105.154 kb on + strand, within GFF1910 at 105.228 kb on + strand, within GFF1910 at 105.229 kb on - strand, within GFF1910 at 105.325 kb on + strand at 105.367 kb on - strand at 105.381 kb on + strand at 105.417 kb on + strand at 105.417 kb on + strand at 105.545 kb on + strand, within GFF1911 at 105.582 kb on + strand, within GFF1911 at 105.582 kb on + strand, within GFF1911 at 105.812 kb on + strand, within GFF1911 at 105.859 kb on + strand, within GFF1911 at 106.290 kb on - strand, within GFF1912 at 106.390 kb on + strand, within GFF1912 at 106.390 kb on + strand, within GFF1912 at 106.391 kb on - strand, within GFF1912 at 106.391 kb on - strand, within GFF1912 at 106.391 kb on - strand, within GFF1912 at 106.429 kb on - strand, within GFF1912 at 106.655 kb on - strand at 106.655 kb on - strand at 106.655 kb on - strand at 106.678 kb on + strand at 106.679 kb on + strand at 106.682 kb on + strand at 106.701 kb on + strand at 106.899 kb on - strand, within GFF1913 at 106.937 kb on + strand, within GFF1913
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 104,423 + GFF1909 0.64 -1.3 104,423 + GFF1909 0.64 -0.6 104,423 + GFF1909 0.64 -0.8 104,424 - GFF1909 0.64 -1.4 104,424 - GFF1909 0.64 -0.4 104,444 + GFF1909 0.66 +0.5 104,531 - GFF1909 0.74 -1.8 104,563 + GFF1909 0.77 +3.1 104,770 + -1.1 104,770 + -1.5 104,770 + +0.1 104,770 + +2.2 104,771 - -0.2 105,067 + GFF1910 0.48 +3.9 105,149 + GFF1910 0.63 -0.8 105,150 - GFF1910 0.63 +0.2 105,150 - GFF1910 0.63 +1.1 105,154 + GFF1910 0.64 +0.1 105,228 + GFF1910 0.77 +0.3 105,229 - GFF1910 0.78 +0.8 105,325 + +0.0 105,367 - -0.6 105,381 + +0.7 105,417 + -2.5 105,417 + -2.2 105,545 + GFF1911 0.25 +0.1 105,582 + GFF1911 0.31 +0.8 105,582 + GFF1911 0.31 -0.6 105,812 + GFF1911 0.73 +0.3 105,859 + GFF1911 0.82 -0.8 106,290 - GFF1912 0.42 -0.9 106,390 + GFF1912 0.58 -1.9 106,390 + GFF1912 0.58 -0.1 106,391 - GFF1912 0.58 +0.2 106,391 - GFF1912 0.58 -0.0 106,391 - GFF1912 0.58 -0.5 106,429 - GFF1912 0.64 -0.6 106,655 - +0.7 106,655 - +0.2 106,655 - +0.2 106,678 + +2.7 106,679 + +0.3 106,682 + +0.3 106,701 + -0.3 106,899 - GFF1913 0.15 +0.5 106,937 + GFF1913 0.21 -1.8
Or see this region's nucleotide sequence