Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1708 and GFF1709 are separated by 7 nucleotides GFF1709 and GFF1710 are separated by 196 nucleotides
GFF1708 - Ribose ABC transport system, ATP-binding protein RbsA (TC 3.A.1.2.1), at 6,248 to 7,753
GFF1708
GFF1709 - Ribose ABC transport system, high affinity permease RbsD (TC 3.A.1.2.1), at 7,761 to 8,180
GFF1709
GFF1710 - Kup system potassium uptake protein, at 8,377 to 10,245
GFF1710
Position (kb)
7
8
9 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 6.762 kb on + strand, within GFF1708 at 6.763 kb on - strand, within GFF1708 at 6.831 kb on + strand, within GFF1708 at 6.831 kb on + strand, within GFF1708 at 7.033 kb on + strand, within GFF1708 at 7.154 kb on - strand, within GFF1708 at 7.166 kb on - strand, within GFF1708 at 7.167 kb on + strand, within GFF1708 at 7.167 kb on + strand, within GFF1708 at 7.168 kb on - strand, within GFF1708 at 7.168 kb on - strand, within GFF1708 at 7.168 kb on - strand, within GFF1708 at 7.168 kb on - strand, within GFF1708 at 7.168 kb on - strand, within GFF1708 at 7.505 kb on - strand, within GFF1708 at 7.593 kb on + strand, within GFF1708 at 7.593 kb on + strand, within GFF1708 at 7.594 kb on - strand, within GFF1708 at 7.605 kb on - strand at 7.720 kb on + strand at 8.027 kb on + strand, within GFF1709 at 8.028 kb on - strand, within GFF1709 at 8.115 kb on - strand, within GFF1709 at 8.121 kb on - strand, within GFF1709 at 8.163 kb on + strand at 8.163 kb on + strand at 8.222 kb on - strand at 8.240 kb on - strand at 8.307 kb on + strand at 8.322 kb on + strand at 8.394 kb on + strand at 8.399 kb on - strand at 8.496 kb on - strand at 8.535 kb on + strand at 8.565 kb on + strand, within GFF1710 at 8.565 kb on + strand, within GFF1710 at 8.566 kb on - strand, within GFF1710 at 8.566 kb on - strand, within GFF1710 at 8.689 kb on - strand, within GFF1710 at 8.696 kb on + strand, within GFF1710 at 8.696 kb on + strand, within GFF1710 at 8.773 kb on + strand, within GFF1710 at 8.810 kb on + strand, within GFF1710 at 8.810 kb on + strand, within GFF1710 at 8.810 kb on + strand, within GFF1710 at 8.810 kb on + strand, within GFF1710 at 8.811 kb on - strand, within GFF1710 at 8.811 kb on - strand, within GFF1710 at 8.811 kb on - strand, within GFF1710 at 8.811 kb on - strand, within GFF1710 at 8.811 kb on - strand, within GFF1710 at 8.811 kb on - strand, within GFF1710 at 8.813 kb on - strand, within GFF1710 at 8.895 kb on + strand, within GFF1710 at 8.994 kb on + strand, within GFF1710 at 9.063 kb on - strand, within GFF1710 at 9.085 kb on - strand, within GFF1710 at 9.108 kb on + strand, within GFF1710 at 9.147 kb on - strand, within GFF1710
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 6,762 + GFF1708 0.34 +0.6 6,763 - GFF1708 0.34 -0.6 6,831 + GFF1708 0.39 +0.4 6,831 + GFF1708 0.39 -1.6 7,033 + GFF1708 0.52 +0.2 7,154 - GFF1708 0.60 +1.5 7,166 - GFF1708 0.61 +1.6 7,167 + GFF1708 0.61 -0.2 7,167 + GFF1708 0.61 +0.1 7,168 - GFF1708 0.61 +0.5 7,168 - GFF1708 0.61 -0.5 7,168 - GFF1708 0.61 -0.5 7,168 - GFF1708 0.61 +0.8 7,168 - GFF1708 0.61 +2.0 7,505 - GFF1708 0.83 -0.2 7,593 + GFF1708 0.89 -0.8 7,593 + GFF1708 0.89 -0.2 7,594 - GFF1708 0.89 -0.1 7,605 - +1.2 7,720 + +0.0 8,027 + GFF1709 0.63 -0.2 8,028 - GFF1709 0.64 -1.6 8,115 - GFF1709 0.84 -0.3 8,121 - GFF1709 0.86 +0.4 8,163 + +1.5 8,163 + -0.4 8,222 - -0.6 8,240 - +4.0 8,307 + +2.0 8,322 + +1.1 8,394 + +0.5 8,399 - -0.3 8,496 - +0.5 8,535 + -0.5 8,565 + GFF1710 0.10 -0.2 8,565 + GFF1710 0.10 +0.7 8,566 - GFF1710 0.10 +0.1 8,566 - GFF1710 0.10 +2.8 8,689 - GFF1710 0.17 -0.5 8,696 + GFF1710 0.17 -0.3 8,696 + GFF1710 0.17 +1.1 8,773 + GFF1710 0.21 +2.1 8,810 + GFF1710 0.23 +0.3 8,810 + GFF1710 0.23 +0.2 8,810 + GFF1710 0.23 -0.3 8,810 + GFF1710 0.23 -0.2 8,811 - GFF1710 0.23 -0.6 8,811 - GFF1710 0.23 +0.2 8,811 - GFF1710 0.23 -0.8 8,811 - GFF1710 0.23 -0.6 8,811 - GFF1710 0.23 +0.0 8,811 - GFF1710 0.23 -0.9 8,813 - GFF1710 0.23 +0.1 8,895 + GFF1710 0.28 -1.0 8,994 + GFF1710 0.33 -0.3 9,063 - GFF1710 0.37 +0.4 9,085 - GFF1710 0.38 -1.2 9,108 + GFF1710 0.39 +0.2 9,147 - GFF1710 0.41 +0.1
Or see this region's nucleotide sequence