Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1646

Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1644 and GFF1645 are separated by 36 nucleotidesGFF1645 and GFF1646 are separated by 29 nucleotidesGFF1646 and GFF1647 are separated by 102 nucleotides GFF1644 - Crossover junction endodeoxyribonuclease RuvC (EC 3.1.22.4), at 29,649 to 30,170 GFF1644 GFF1645 - FIG000859: hypothetical protein YebC, at 30,207 to 30,947 GFF1645 GFF1646 - Dihydroneopterin triphosphate pyrophosphohydrolase type 2 (nudB), at 30,977 to 31,429 GFF1646 GFF1647 - Aspartyl-tRNA synthetase (EC 6.1.1.12), at 31,532 to 33,304 GFF1647 Position (kb) 30 31 32Strain fitness (log2 ratio) -1 0 1at 30.455 kb on - strand, within GFF1645at 30.455 kb on - strand, within GFF1645at 30.657 kb on - strand, within GFF1645at 30.689 kb on - strand, within GFF1645at 30.689 kb on - strand, within GFF1645at 30.933 kb on - strandat 30.933 kb on - strandat 30.936 kb on - strandat 31.089 kb on - strand, within GFF1646at 31.520 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
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30,455 - GFF1645 0.33 +0.5
30,455 - GFF1645 0.33 +0.1
30,657 - GFF1645 0.61 -1.3
30,689 - GFF1645 0.65 +0.6
30,689 - GFF1645 0.65 -0.5
30,933 - +0.1
30,933 - -0.4
30,936 - -0.8
31,089 - GFF1646 0.25 -0.7
31,520 - +0.9

Or see this region's nucleotide sequence