Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF158 and GFF159 are separated by 171 nucleotides GFF159 and GFF160 overlap by 11 nucleotides GFF160 and GFF161 overlap by 1 nucleotides GFF161 and GFF162 are separated by 384 nucleotides
GFF158 - Fructose repressor FruR, LacI family, at 149,749 to 150,753
GFF158
GFF159 - hypothetical protein, at 150,925 to 151,047
GFF159
GFF160 - Acetolactate synthase small subunit (EC 2.2.1.6), at 151,037 to 151,531
GFF160
GFF161 - Acetolactate synthase large subunit (EC 2.2.1.6), at 151,531 to 153,192
GFF161
GFF162 - Probable transcriptional activator for leuABCD operon, at 153,577 to 154,521
GFF162
Position (kb)
151
152
153
154 Strain fitness (log2 ratio)
-1
0
1
2
3 at 150.614 kb on + strand, within GFF158 at 150.614 kb on + strand, within GFF158 at 150.672 kb on - strand at 150.699 kb on - strand at 150.699 kb on - strand at 150.775 kb on + strand at 150.822 kb on + strand at 150.823 kb on - strand at 150.916 kb on + strand at 150.984 kb on - strand, within GFF159 at 150.993 kb on + strand, within GFF159 at 151.169 kb on - strand, within GFF160 at 151.171 kb on - strand, within GFF160 at 151.219 kb on + strand, within GFF160 at 151.444 kb on + strand, within GFF160 at 151.445 kb on - strand, within GFF160 at 151.447 kb on - strand, within GFF160 at 151.508 kb on + strand at 151.648 kb on - strand at 151.724 kb on + strand, within GFF161 at 151.725 kb on - strand, within GFF161 at 151.765 kb on - strand, within GFF161 at 151.767 kb on - strand, within GFF161 at 151.767 kb on - strand, within GFF161 at 151.791 kb on - strand, within GFF161 at 151.791 kb on - strand, within GFF161 at 151.791 kb on - strand, within GFF161 at 151.791 kb on - strand, within GFF161 at 151.798 kb on - strand, within GFF161 at 151.870 kb on + strand, within GFF161 at 151.870 kb on + strand, within GFF161 at 151.870 kb on + strand, within GFF161 at 151.903 kb on + strand, within GFF161 at 151.903 kb on + strand, within GFF161 at 151.905 kb on + strand, within GFF161 at 151.905 kb on + strand, within GFF161 at 152.010 kb on + strand, within GFF161 at 152.042 kb on + strand, within GFF161 at 152.043 kb on - strand, within GFF161 at 152.071 kb on + strand, within GFF161 at 152.087 kb on + strand, within GFF161 at 152.087 kb on + strand, within GFF161 at 152.088 kb on - strand, within GFF161 at 152.218 kb on - strand, within GFF161 at 152.254 kb on + strand, within GFF161 at 152.255 kb on - strand, within GFF161 at 152.353 kb on + strand, within GFF161 at 152.353 kb on + strand, within GFF161 at 152.375 kb on + strand, within GFF161 at 152.376 kb on - strand, within GFF161 at 152.443 kb on - strand, within GFF161 at 152.465 kb on + strand, within GFF161 at 152.465 kb on + strand, within GFF161 at 152.466 kb on - strand, within GFF161 at 152.589 kb on - strand, within GFF161 at 152.622 kb on - strand, within GFF161 at 152.679 kb on + strand, within GFF161 at 152.680 kb on - strand, within GFF161 at 152.680 kb on - strand, within GFF161 at 152.702 kb on + strand, within GFF161 at 152.736 kb on - strand, within GFF161 at 152.764 kb on - strand, within GFF161 at 152.871 kb on + strand, within GFF161 at 152.871 kb on + strand, within GFF161 at 152.872 kb on - strand, within GFF161 at 152.872 kb on - strand, within GFF161 at 152.872 kb on - strand, within GFF161 at 152.872 kb on - strand, within GFF161 at 152.928 kb on - strand, within GFF161 at 152.987 kb on + strand, within GFF161 at 153.145 kb on + strand at 153.146 kb on - strand at 153.155 kb on + strand at 153.155 kb on + strand at 153.272 kb on - strand at 153.627 kb on + strand at 153.698 kb on - strand, within GFF162 at 153.856 kb on - strand, within GFF162 at 153.901 kb on - strand, within GFF162
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 150,614 + GFF158 0.86 -0.5 150,614 + GFF158 0.86 -1.2 150,672 - -0.7 150,699 - -1.2 150,699 - -1.1 150,775 + -0.7 150,822 + +0.3 150,823 - -0.3 150,916 + -0.0 150,984 - GFF159 0.48 +0.5 150,993 + GFF159 0.55 +0.1 151,169 - GFF160 0.27 +0.3 151,171 - GFF160 0.27 -1.0 151,219 + GFF160 0.37 -0.5 151,444 + GFF160 0.82 -0.1 151,445 - GFF160 0.82 +3.5 151,447 - GFF160 0.83 +0.1 151,508 + -0.5 151,648 - +3.4 151,724 + GFF161 0.12 -0.2 151,725 - GFF161 0.12 +1.1 151,765 - GFF161 0.14 +0.1 151,767 - GFF161 0.14 +0.5 151,767 - GFF161 0.14 +0.1 151,791 - GFF161 0.16 -1.6 151,791 - GFF161 0.16 +1.6 151,791 - GFF161 0.16 -0.2 151,791 - GFF161 0.16 +0.9 151,798 - GFF161 0.16 +0.9 151,870 + GFF161 0.20 -0.2 151,870 + GFF161 0.20 -0.0 151,870 + GFF161 0.20 +0.5 151,903 + GFF161 0.22 +1.1 151,903 + GFF161 0.22 -0.3 151,905 + GFF161 0.23 +0.3 151,905 + GFF161 0.23 -0.1 152,010 + GFF161 0.29 -0.6 152,042 + GFF161 0.31 -1.0 152,043 - GFF161 0.31 +0.8 152,071 + GFF161 0.32 +0.3 152,087 + GFF161 0.33 +0.0 152,087 + GFF161 0.33 -0.7 152,088 - GFF161 0.34 +0.3 152,218 - GFF161 0.41 +0.1 152,254 + GFF161 0.44 +0.9 152,255 - GFF161 0.44 -0.7 152,353 + GFF161 0.49 +0.9 152,353 + GFF161 0.49 -0.1 152,375 + GFF161 0.51 +1.8 152,376 - GFF161 0.51 +0.4 152,443 - GFF161 0.55 -0.4 152,465 + GFF161 0.56 -0.6 152,465 + GFF161 0.56 -0.3 152,466 - GFF161 0.56 +1.5 152,589 - GFF161 0.64 +0.6 152,622 - GFF161 0.66 +0.3 152,679 + GFF161 0.69 -0.4 152,680 - GFF161 0.69 +0.6 152,680 - GFF161 0.69 +0.6 152,702 + GFF161 0.70 +0.4 152,736 - GFF161 0.73 +0.2 152,764 - GFF161 0.74 -0.2 152,871 + GFF161 0.81 +0.5 152,871 + GFF161 0.81 -0.8 152,872 - GFF161 0.81 -0.3 152,872 - GFF161 0.81 +0.7 152,872 - GFF161 0.81 -0.7 152,872 - GFF161 0.81 -1.0 152,928 - GFF161 0.84 +0.7 152,987 + GFF161 0.88 +1.0 153,145 + -0.3 153,146 - +0.4 153,155 + +0.3 153,155 + -0.4 153,272 - +1.7 153,627 + -0.1 153,698 - GFF162 0.13 -0.0 153,856 - GFF162 0.30 +0.3 153,901 - GFF162 0.34 -0.2
Or see this region's nucleotide sequence