Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1468 and GFF1469 are separated by 0 nucleotides GFF1469 and GFF1470 overlap by 4 nucleotides GFF1470 and GFF1471 are separated by 133 nucleotides
GFF1468 - Sensor protein basS/pmrB (EC 2.7.3.-), at 132,770 to 133,849
GFF1468
GFF1469 - Transcriptional regulatory protein basR/pmrA, at 133,850 to 134,518
GFF1469
GFF1470 - Phosphoethanolamine transferase EptA specific for the 1 phosphate group of core-lipid A, at 134,515 to 136,158
GFF1470
GFF1471 - Arginine/agmatine antiporter, at 136,292 to 137,560
GFF1471
Position (kb)
134
135
136
137 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 133.517 kb on - strand, within GFF1468 at 133.615 kb on + strand, within GFF1468 at 133.616 kb on - strand, within GFF1468 at 133.669 kb on + strand, within GFF1468 at 133.754 kb on - strand at 133.754 kb on - strand at 133.780 kb on + strand at 133.851 kb on + strand at 133.878 kb on + strand at 133.933 kb on + strand, within GFF1469 at 133.933 kb on + strand, within GFF1469 at 133.937 kb on + strand, within GFF1469 at 133.937 kb on + strand, within GFF1469 at 133.938 kb on - strand, within GFF1469 at 133.938 kb on - strand, within GFF1469 at 133.938 kb on - strand, within GFF1469 at 133.939 kb on + strand, within GFF1469 at 133.939 kb on + strand, within GFF1469 at 133.940 kb on - strand, within GFF1469 at 133.984 kb on - strand, within GFF1469 at 133.984 kb on - strand, within GFF1469 at 133.993 kb on + strand, within GFF1469 at 133.994 kb on - strand, within GFF1469 at 133.995 kb on + strand, within GFF1469 at 134.056 kb on - strand, within GFF1469 at 134.111 kb on + strand, within GFF1469 at 134.112 kb on - strand, within GFF1469 at 134.114 kb on - strand, within GFF1469 at 134.114 kb on - strand, within GFF1469 at 134.162 kb on - strand, within GFF1469 at 134.307 kb on + strand, within GFF1469 at 134.404 kb on + strand, within GFF1469 at 134.469 kb on - strand at 134.483 kb on + strand at 134.484 kb on - strand at 134.567 kb on + strand at 134.568 kb on - strand at 134.579 kb on - strand at 134.673 kb on - strand at 134.805 kb on - strand, within GFF1470 at 134.866 kb on + strand, within GFF1470 at 134.868 kb on + strand, within GFF1470 at 134.869 kb on - strand, within GFF1470 at 134.871 kb on - strand, within GFF1470 at 134.871 kb on - strand, within GFF1470 at 134.871 kb on - strand, within GFF1470 at 134.892 kb on + strand, within GFF1470 at 134.897 kb on + strand, within GFF1470 at 134.898 kb on - strand, within GFF1470 at 134.990 kb on + strand, within GFF1470 at 134.990 kb on + strand, within GFF1470 at 134.990 kb on + strand, within GFF1470 at 135.019 kb on - strand, within GFF1470 at 135.019 kb on - strand, within GFF1470 at 135.023 kb on - strand, within GFF1470 at 135.057 kb on + strand, within GFF1470 at 135.058 kb on - strand, within GFF1470 at 135.059 kb on + strand, within GFF1470 at 135.109 kb on - strand, within GFF1470 at 135.128 kb on - strand, within GFF1470 at 135.199 kb on + strand, within GFF1470 at 135.225 kb on + strand, within GFF1470 at 135.226 kb on - strand, within GFF1470 at 135.266 kb on + strand, within GFF1470 at 135.283 kb on - strand, within GFF1470 at 135.348 kb on - strand, within GFF1470 at 135.509 kb on - strand, within GFF1470 at 135.542 kb on + strand, within GFF1470 at 135.542 kb on + strand, within GFF1470 at 135.564 kb on + strand, within GFF1470 at 135.565 kb on - strand, within GFF1470 at 135.624 kb on - strand, within GFF1470 at 135.702 kb on + strand, within GFF1470 at 135.706 kb on + strand, within GFF1470 at 135.707 kb on - strand, within GFF1470 at 135.970 kb on - strand, within GFF1470 at 135.982 kb on - strand, within GFF1470 at 136.033 kb on + strand at 136.040 kb on - strand at 136.040 kb on - strand at 136.049 kb on - strand at 136.052 kb on - strand at 136.052 kb on - strand at 136.061 kb on + strand at 136.061 kb on + strand at 136.089 kb on + strand at 136.090 kb on - strand at 136.091 kb on + strand at 136.092 kb on - strand at 136.153 kb on + strand at 136.154 kb on - strand at 136.219 kb on - strand at 136.227 kb on - strand at 136.279 kb on + strand at 136.279 kb on + strand at 136.280 kb on - strand at 136.280 kb on - strand at 136.280 kb on - strand at 136.280 kb on - strand at 136.280 kb on - strand at 136.280 kb on - strand at 136.284 kb on - strand at 136.324 kb on - strand at 136.347 kb on + strand at 136.348 kb on - strand at 136.359 kb on + strand at 136.360 kb on - strand at 136.441 kb on - strand, within GFF1471 at 136.459 kb on + strand, within GFF1471 at 136.511 kb on + strand, within GFF1471 at 136.828 kb on + strand, within GFF1471 at 136.915 kb on - strand, within GFF1471 at 137.096 kb on - strand, within GFF1471
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 133,517 - GFF1468 0.69 +0.5 133,615 + GFF1468 0.78 -0.4 133,616 - GFF1468 0.78 +0.2 133,669 + GFF1468 0.83 +1.2 133,754 - -1.2 133,754 - +0.7 133,780 + -0.2 133,851 + -0.5 133,878 + -0.4 133,933 + GFF1469 0.12 +0.3 133,933 + GFF1469 0.12 +0.5 133,937 + GFF1469 0.13 -0.1 133,937 + GFF1469 0.13 -0.1 133,938 - GFF1469 0.13 +0.2 133,938 - GFF1469 0.13 +0.2 133,938 - GFF1469 0.13 +1.2 133,939 + GFF1469 0.13 -0.0 133,939 + GFF1469 0.13 +0.7 133,940 - GFF1469 0.13 +1.0 133,984 - GFF1469 0.20 +0.6 133,984 - GFF1469 0.20 +0.5 133,993 + GFF1469 0.21 +0.5 133,994 - GFF1469 0.22 -0.4 133,995 + GFF1469 0.22 -0.6 134,056 - GFF1469 0.31 -0.1 134,111 + GFF1469 0.39 +0.0 134,112 - GFF1469 0.39 -0.2 134,114 - GFF1469 0.39 +0.6 134,114 - GFF1469 0.39 -0.1 134,162 - GFF1469 0.47 -0.1 134,307 + GFF1469 0.68 +2.4 134,404 + GFF1469 0.83 +1.7 134,469 - -0.9 134,483 + +0.7 134,484 - +1.0 134,567 + +0.6 134,568 - +1.9 134,579 - -1.1 134,673 - -0.4 134,805 - GFF1470 0.18 +0.4 134,866 + GFF1470 0.21 +0.3 134,868 + GFF1470 0.21 +3.0 134,869 - GFF1470 0.22 -0.1 134,871 - GFF1470 0.22 -0.6 134,871 - GFF1470 0.22 +0.1 134,871 - GFF1470 0.22 -0.7 134,892 + GFF1470 0.23 +0.0 134,897 + GFF1470 0.23 +1.3 134,898 - GFF1470 0.23 +0.0 134,990 + GFF1470 0.29 -0.4 134,990 + GFF1470 0.29 -0.2 134,990 + GFF1470 0.29 +0.6 135,019 - GFF1470 0.31 -0.1 135,019 - GFF1470 0.31 -1.3 135,023 - GFF1470 0.31 -0.2 135,057 + GFF1470 0.33 +0.4 135,058 - GFF1470 0.33 -0.2 135,059 + GFF1470 0.33 +0.1 135,109 - GFF1470 0.36 +1.4 135,128 - GFF1470 0.37 -0.3 135,199 + GFF1470 0.42 -0.7 135,225 + GFF1470 0.43 -0.1 135,226 - GFF1470 0.43 -0.2 135,266 + GFF1470 0.46 +0.6 135,283 - GFF1470 0.47 +2.4 135,348 - GFF1470 0.51 +0.1 135,509 - GFF1470 0.60 +1.8 135,542 + GFF1470 0.62 +0.8 135,542 + GFF1470 0.62 -1.2 135,564 + GFF1470 0.64 +0.4 135,565 - GFF1470 0.64 -0.8 135,624 - GFF1470 0.67 -0.8 135,702 + GFF1470 0.72 -0.1 135,706 + GFF1470 0.72 +0.2 135,707 - GFF1470 0.73 +0.4 135,970 - GFF1470 0.89 +1.9 135,982 - GFF1470 0.89 -0.9 136,033 + -0.1 136,040 - -0.2 136,040 - +0.0 136,049 - +0.1 136,052 - +0.4 136,052 - +0.4 136,061 + -1.8 136,061 + +0.1 136,089 + -0.2 136,090 - +0.3 136,091 + +0.3 136,092 - +0.5 136,153 + -0.7 136,154 - -0.2 136,219 - -0.1 136,227 - +0.2 136,279 + -0.7 136,279 + +0.4 136,280 - -0.8 136,280 - -0.3 136,280 - -0.6 136,280 - -0.4 136,280 - -0.3 136,280 - +0.0 136,284 - +0.8 136,324 - -0.0 136,347 + +0.1 136,348 - +0.0 136,359 + -1.0 136,360 - -0.1 136,441 - GFF1471 0.12 -0.1 136,459 + GFF1471 0.13 +0.5 136,511 + GFF1471 0.17 +0.6 136,828 + GFF1471 0.42 -0.5 136,915 - GFF1471 0.49 +1.2 137,096 - GFF1471 0.63 +0.2
Or see this region's nucleotide sequence