Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1389 and GFF1390 overlap by 4 nucleotides GFF1390 and GFF1391 are separated by 159 nucleotides GFF1391 and GFF1392 are separated by 12 nucleotides GFF1392 and GFF1393 are separated by 2 nucleotides GFF1393 and GFF1394 are separated by 575 nucleotides
GFF1389 - FIG074102: hypothetical protein, at 35,511 to 35,708
GFF1389
GFF1390 - Phage protein, at 35,705 to 36,160
GFF1390
GFF1391 - Putative inner membrane protein, at 36,320 to 36,634
GFF1391
GFF1392 - Phage lysin, at 36,647 to 37,252
GFF1392
GFF1393 - Putative inner membrane protein, at 37,255 to 37,542
GFF1393
GFF1394 - putative cytoplasmic protein, at 38,118 to 38,465
GFF1394
Position (kb) 36
37
38 Strain fitness (log2 ratio) -2
-1
0
1 at 35.647 kb on - strand, within GFF1389 at 35.833 kb on - strand, within GFF1390 at 35.919 kb on - strand, within GFF1390 at 35.930 kb on + strand, within GFF1390 at 35.931 kb on - strand, within GFF1390 at 36.095 kb on - strand, within GFF1390 at 36.120 kb on - strand at 36.824 kb on - strand, within GFF1392 at 36.927 kb on + strand, within GFF1392 at 36.928 kb on - strand, within GFF1392 at 37.024 kb on + strand, within GFF1392 at 37.024 kb on + strand, within GFF1392 at 37.091 kb on + strand, within GFF1392 at 37.091 kb on + strand, within GFF1392 at 37.092 kb on - strand, within GFF1392 at 37.235 kb on + strand at 37.236 kb on - strand at 37.236 kb on - strand at 37.236 kb on - strand at 37.239 kb on + strand at 37.240 kb on - strand at 37.384 kb on - strand, within GFF1393 at 37.436 kb on + strand, within GFF1393 at 37.489 kb on + strand, within GFF1393 at 37.489 kb on + strand, within GFF1393 at 37.611 kb on - strand at 38.113 kb on - strand at 38.211 kb on - strand, within GFF1394
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 35,647 - GFF1389 0.69 +0.5 35,833 - GFF1390 0.28 -1.4 35,919 - GFF1390 0.47 +0.1 35,930 + GFF1390 0.49 +0.1 35,931 - GFF1390 0.50 +0.5 36,095 - GFF1390 0.86 +0.6 36,120 - +1.4 36,824 - GFF1392 0.29 +0.9 36,927 + GFF1392 0.46 +1.0 36,928 - GFF1392 0.46 -0.4 37,024 + GFF1392 0.62 -0.1 37,024 + GFF1392 0.62 +0.3 37,091 + GFF1392 0.73 +0.0 37,091 + GFF1392 0.73 +0.2 37,092 - GFF1392 0.73 +1.3 37,235 + -0.4 37,236 - +0.1 37,236 - -0.3 37,236 - -0.2 37,239 + -0.9 37,240 - +0.4 37,384 - GFF1393 0.45 +1.0 37,436 + GFF1393 0.63 +0.4 37,489 + GFF1393 0.81 +1.2 37,489 + GFF1393 0.81 -0.2 37,611 - -2.6 38,113 - +0.5 38,211 - GFF1394 0.27 +0.6
Or see this region's nucleotide sequence