Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1315 and GFF1316 are separated by 169 nucleotides GFF1316 and GFF1317 are separated by 250 nucleotides GFF1317 and GFF1318 are separated by 86 nucleotides
GFF1315 - FIG003462: membrane protein, at 23,066 to 23,779
GFF1315
GFF1316 - Hypothetical lipoprotein ygdR precursor, at 23,949 to 24,167
GFF1316
GFF1317 - Oxidoreductase, aldo/keto reductase family, at 24,418 to 25,398
GFF1317
GFF1318 - Lysophospholipid transporter LplT, at 25,485 to 26,687
GFF1318
Position (kb) 24
25
26 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 23.476 kb on + strand, within GFF1315 at 23.477 kb on - strand, within GFF1315 at 23.530 kb on + strand, within GFF1315 at 23.534 kb on - strand, within GFF1315 at 23.704 kb on + strand, within GFF1315 at 23.704 kb on + strand, within GFF1315 at 23.708 kb on + strand at 23.759 kb on - strand at 23.810 kb on + strand at 23.820 kb on - strand at 23.884 kb on + strand at 23.885 kb on - strand at 23.902 kb on - strand at 23.902 kb on - strand at 23.916 kb on - strand at 23.932 kb on + strand at 23.932 kb on + strand at 23.980 kb on + strand, within GFF1316 at 24.018 kb on + strand, within GFF1316 at 24.022 kb on + strand, within GFF1316 at 24.045 kb on - strand, within GFF1316 at 24.099 kb on + strand, within GFF1316 at 24.099 kb on + strand, within GFF1316 at 24.100 kb on - strand, within GFF1316 at 24.339 kb on + strand at 24.340 kb on - strand at 24.372 kb on + strand at 24.372 kb on + strand at 24.395 kb on + strand at 24.395 kb on + strand at 24.399 kb on + strand at 24.399 kb on + strand at 24.399 kb on + strand at 24.399 kb on + strand at 24.470 kb on - strand at 24.490 kb on - strand at 24.515 kb on - strand at 24.559 kb on + strand, within GFF1317 at 24.560 kb on - strand, within GFF1317 at 24.636 kb on + strand, within GFF1317 at 24.793 kb on + strand, within GFF1317 at 24.794 kb on - strand, within GFF1317 at 24.795 kb on + strand, within GFF1317 at 24.839 kb on + strand, within GFF1317 at 24.849 kb on - strand, within GFF1317 at 24.849 kb on - strand, within GFF1317 at 24.883 kb on - strand, within GFF1317 at 24.883 kb on - strand, within GFF1317 at 24.981 kb on + strand, within GFF1317 at 25.000 kb on + strand, within GFF1317 at 25.031 kb on - strand, within GFF1317 at 25.126 kb on - strand, within GFF1317 at 25.269 kb on + strand, within GFF1317 at 25.269 kb on + strand, within GFF1317 at 25.378 kb on + strand at 25.378 kb on + strand at 25.381 kb on - strand at 25.419 kb on + strand at 25.447 kb on - strand at 25.447 kb on - strand at 25.489 kb on + strand at 25.490 kb on - strand at 25.617 kb on + strand, within GFF1318 at 25.618 kb on - strand, within GFF1318 at 25.620 kb on + strand, within GFF1318 at 25.620 kb on + strand, within GFF1318 at 25.621 kb on - strand, within GFF1318 at 25.763 kb on - strand, within GFF1318 at 26.078 kb on + strand, within GFF1318 at 26.217 kb on + strand, within GFF1318 at 26.326 kb on + strand, within GFF1318 at 26.369 kb on + strand, within GFF1318
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 23,476 + GFF1315 0.57 +0.4 23,477 - GFF1315 0.58 -0.2 23,530 + GFF1315 0.65 -0.5 23,534 - GFF1315 0.66 +0.9 23,704 + GFF1315 0.89 +2.4 23,704 + GFF1315 0.89 +0.1 23,708 + +2.7 23,759 - -0.7 23,810 + +0.4 23,820 - +0.4 23,884 + +0.7 23,885 - +0.2 23,902 - +0.3 23,902 - -0.0 23,916 - -0.3 23,932 + -0.7 23,932 + +0.2 23,980 + GFF1316 0.14 -0.3 24,018 + GFF1316 0.32 +0.1 24,022 + GFF1316 0.33 +2.3 24,045 - GFF1316 0.44 -0.1 24,099 + GFF1316 0.68 +0.4 24,099 + GFF1316 0.68 -0.6 24,100 - GFF1316 0.69 +0.5 24,339 + +1.5 24,340 - +0.9 24,372 + +0.6 24,372 + -1.0 24,395 + +0.2 24,395 + +0.7 24,399 + +3.0 24,399 + +0.7 24,399 + -0.5 24,399 + +1.1 24,470 - +0.4 24,490 - -1.6 24,515 - -0.9 24,559 + GFF1317 0.14 -1.2 24,560 - GFF1317 0.14 +0.0 24,636 + GFF1317 0.22 +1.0 24,793 + GFF1317 0.38 -0.7 24,794 - GFF1317 0.38 -0.2 24,795 + GFF1317 0.38 +0.3 24,839 + GFF1317 0.43 +1.8 24,849 - GFF1317 0.44 +0.3 24,849 - GFF1317 0.44 -0.2 24,883 - GFF1317 0.47 -0.6 24,883 - GFF1317 0.47 +0.6 24,981 + GFF1317 0.57 -0.1 25,000 + GFF1317 0.59 +0.0 25,031 - GFF1317 0.62 -0.0 25,126 - GFF1317 0.72 +1.4 25,269 + GFF1317 0.87 +0.8 25,269 + GFF1317 0.87 +0.5 25,378 + +0.1 25,378 + +0.2 25,381 - -0.9 25,419 + -0.8 25,447 - -0.7 25,447 - +0.7 25,489 + -0.3 25,490 - +1.6 25,617 + GFF1318 0.11 -0.6 25,618 - GFF1318 0.11 -2.6 25,620 + GFF1318 0.11 -1.3 25,620 + GFF1318 0.11 -0.1 25,621 - GFF1318 0.11 -0.4 25,763 - GFF1318 0.23 -0.1 26,078 + GFF1318 0.49 +0.7 26,217 + GFF1318 0.61 +0.5 26,326 + GFF1318 0.70 +1.1 26,369 + GFF1318 0.73 +0.4
Or see this region's nucleotide sequence