Experiment: LB_plus_SM_buffer with 6F2_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1243 and GFF1244 overlap by 11 nucleotides GFF1244 and GFF1245 are separated by 36 nucleotides GFF1245 and GFF1246 are separated by 421 nucleotides
GFF1243 - COG3267: Type II secretory pathway, component ExeA (predicted ATPase), at 74,372 to 75,340
GFF1243
GFF1244 - Putative integrase protein, at 75,330 to 75,881
GFF1244
GFF1245 - FIG01046087: hypothetical protein, at 75,918 to 76,556
GFF1245
GFF1246 - RlgA, at 76,978 to 77,400
GFF1246
Position (kb)
75
76
77 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 74.923 kb on + strand, within GFF1243 at 74.924 kb on - strand, within GFF1243 at 74.926 kb on + strand, within GFF1243 at 74.926 kb on + strand, within GFF1243 at 75.061 kb on - strand, within GFF1243 at 75.061 kb on - strand, within GFF1243 at 75.184 kb on + strand, within GFF1243 at 75.262 kb on + strand at 75.262 kb on + strand at 75.279 kb on + strand at 75.282 kb on + strand at 75.298 kb on + strand at 75.371 kb on - strand at 75.391 kb on - strand, within GFF1244 at 75.422 kb on + strand, within GFF1244 at 75.432 kb on - strand, within GFF1244 at 75.659 kb on + strand, within GFF1244 at 75.739 kb on + strand, within GFF1244 at 75.772 kb on + strand, within GFF1244 at 75.772 kb on + strand, within GFF1244 at 75.772 kb on + strand, within GFF1244 at 75.772 kb on + strand, within GFF1244 at 75.772 kb on + strand, within GFF1244 at 75.773 kb on - strand, within GFF1244 at 75.773 kb on - strand, within GFF1244 at 75.773 kb on - strand, within GFF1244 at 75.773 kb on - strand, within GFF1244 at 75.773 kb on - strand, within GFF1244 at 75.773 kb on - strand, within GFF1244 at 75.773 kb on - strand, within GFF1244 at 75.773 kb on - strand, within GFF1244 at 75.916 kb on + strand at 75.916 kb on + strand at 75.916 kb on + strand at 75.916 kb on + strand at 75.917 kb on - strand at 75.917 kb on - strand at 75.936 kb on - strand at 75.936 kb on - strand at 75.936 kb on - strand at 76.018 kb on + strand, within GFF1245 at 76.031 kb on - strand, within GFF1245 at 76.182 kb on - strand, within GFF1245 at 76.227 kb on + strand, within GFF1245 at 76.227 kb on + strand, within GFF1245 at 76.227 kb on + strand, within GFF1245 at 76.228 kb on - strand, within GFF1245 at 76.262 kb on - strand, within GFF1245 at 76.262 kb on - strand, within GFF1245 at 76.360 kb on + strand, within GFF1245 at 76.360 kb on + strand, within GFF1245 at 76.361 kb on - strand, within GFF1245 at 76.372 kb on + strand, within GFF1245 at 76.408 kb on - strand, within GFF1245 at 76.494 kb on - strand at 76.555 kb on - strand at 76.617 kb on + strand at 76.634 kb on + strand at 76.729 kb on - strand at 76.869 kb on - strand at 76.873 kb on + strand at 76.873 kb on + strand at 76.873 kb on + strand at 76.947 kb on + strand at 76.947 kb on + strand at 77.012 kb on + strand at 77.076 kb on + strand, within GFF1246 at 77.078 kb on + strand, within GFF1246 at 77.078 kb on + strand, within GFF1246 at 77.079 kb on - strand, within GFF1246 at 77.079 kb on - strand, within GFF1246 at 77.087 kb on + strand, within GFF1246 at 77.088 kb on - strand, within GFF1246 at 77.090 kb on - strand, within GFF1246 at 77.090 kb on - strand, within GFF1246 at 77.090 kb on - strand, within GFF1246 at 77.105 kb on - strand, within GFF1246 at 77.105 kb on - strand, within GFF1246 at 77.127 kb on - strand, within GFF1246 at 77.128 kb on + strand, within GFF1246 at 77.215 kb on - strand, within GFF1246 at 77.300 kb on + strand, within GFF1246 at 77.300 kb on + strand, within GFF1246 at 77.300 kb on + strand, within GFF1246 at 77.301 kb on - strand, within GFF1246 at 77.301 kb on - strand, within GFF1246 at 77.365 kb on - strand at 77.449 kb on - strand at 77.491 kb on - strand at 77.528 kb on + strand at 77.529 kb on - strand at 77.539 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.1875 MOI remove 74,923 + GFF1243 0.57 -0.6 74,924 - GFF1243 0.57 +1.4 74,926 + GFF1243 0.57 -0.6 74,926 + GFF1243 0.57 +2.4 75,061 - GFF1243 0.71 -0.6 75,061 - GFF1243 0.71 -0.1 75,184 + GFF1243 0.84 +2.3 75,262 + -0.4 75,262 + +1.3 75,279 + +0.9 75,282 + -0.7 75,298 + +0.1 75,371 - -1.0 75,391 - GFF1244 0.11 +4.1 75,422 + GFF1244 0.17 -0.2 75,432 - GFF1244 0.18 -0.8 75,659 + GFF1244 0.60 -0.6 75,739 + GFF1244 0.74 -0.5 75,772 + GFF1244 0.80 +0.6 75,772 + GFF1244 0.80 +0.8 75,772 + GFF1244 0.80 -1.0 75,772 + GFF1244 0.80 -0.1 75,772 + GFF1244 0.80 +0.9 75,773 - GFF1244 0.80 +0.1 75,773 - GFF1244 0.80 +0.4 75,773 - GFF1244 0.80 -1.4 75,773 - GFF1244 0.80 -0.3 75,773 - GFF1244 0.80 +5.5 75,773 - GFF1244 0.80 +0.5 75,773 - GFF1244 0.80 +0.0 75,773 - GFF1244 0.80 +1.6 75,916 + +2.5 75,916 + -0.7 75,916 + -1.1 75,916 + -0.2 75,917 - +0.7 75,917 - -0.6 75,936 - -0.7 75,936 - -1.7 75,936 - -0.7 76,018 + GFF1245 0.16 -0.0 76,031 - GFF1245 0.18 -1.6 76,182 - GFF1245 0.41 -1.4 76,227 + GFF1245 0.48 -0.4 76,227 + GFF1245 0.48 -0.9 76,227 + GFF1245 0.48 -0.6 76,228 - GFF1245 0.49 -0.5 76,262 - GFF1245 0.54 -0.8 76,262 - GFF1245 0.54 -0.1 76,360 + GFF1245 0.69 +0.1 76,360 + GFF1245 0.69 -0.2 76,361 - GFF1245 0.69 -1.6 76,372 + GFF1245 0.71 -0.7 76,408 - GFF1245 0.77 -0.2 76,494 - -0.5 76,555 - +0.1 76,617 + -0.6 76,634 + -0.3 76,729 - +0.6 76,869 - -0.4 76,873 + +0.7 76,873 + -1.1 76,873 + -0.5 76,947 + +0.6 76,947 + +0.1 77,012 + +0.1 77,076 + GFF1246 0.23 -0.9 77,078 + GFF1246 0.24 +0.4 77,078 + GFF1246 0.24 -0.8 77,079 - GFF1246 0.24 +0.8 77,079 - GFF1246 0.24 -0.3 77,087 + GFF1246 0.26 +1.7 77,088 - GFF1246 0.26 +1.0 77,090 - GFF1246 0.26 +0.2 77,090 - GFF1246 0.26 -0.1 77,090 - GFF1246 0.26 -1.4 77,105 - GFF1246 0.30 -0.9 77,105 - GFF1246 0.30 +0.2 77,127 - GFF1246 0.35 -0.7 77,128 + GFF1246 0.35 +0.3 77,215 - GFF1246 0.56 -0.1 77,300 + GFF1246 0.76 +1.2 77,300 + GFF1246 0.76 +1.9 77,300 + GFF1246 0.76 -0.3 77,301 - GFF1246 0.76 -1.9 77,301 - GFF1246 0.76 +1.4 77,365 - +1.3 77,449 - +0.2 77,491 - +0.9 77,528 + -0.4 77,529 - +0.1 77,539 - +0.9
Or see this region's nucleotide sequence